A5083785926- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- melanin and skin pigmentation
- Insect symbiosis and bacterial influences
- Molecular Biology Techniques and Applications
- Genetic and phenotypic traits in livestock
- RNA regulation and disease
- Effects of Environmental Stressors on Livestock
Livestock Improvement Corporation
2022
Massey University
2019-2021
Abstract Background High-producing Holstein Friesian dairy cattle have a characteristic black and white coat, often with large proportions of black. Compared to light coat color, absorbs more solar radiation which is contributing factor heat stress in cattle. To better adapt rapidly warming climates, we aimed lighten their color by genome editing. Results Using gRNA/Cas9-mediated editing, introduced three bp deletion the pre-melanosomal protein 17 gene ( PMEL ) proposed as causative variant...
White spotting of the coat is a characteristic trait various domestic species including cattle and other mammals. It hallmark Holstein-Friesian cattle, several previous studies have detected genetic loci with major effects for white in animals ancestry. Here, our aim was to better understand underlying molecular mechanisms spotting, by conducting largest mapping study this date.Using imputed whole-genome sequence data, we conducted genome-wide association analysis 2973 mixed-breed cows...
Abstract Background Animal health and welfare are at the forefront of public concern agricultural sector is responding by prioritising selection welfare-relevant traits in their breeding schemes. In some cases, welfare-enhancing such as horn-status (i.e., polled) or diluted coat colour, which could enhance heat tolerance, may not segregate breeds primary interest, highlighting gene-editing tools CRISPR-Cas9 technology an approach to rapidly introduce variation into these populations. A major...
Abstract High-producing Holstein Friesian dairy cattle have a characteristic black and white coat pattern where frequently comprises large proportion of the coat. Compared to light color, absorbs more solar radiation translating into radiative heat gain which is contributing factor stress in cattle, negatively impacting on their production levels, fertility welfare. To better adapt rapidly changing climatic conditions with predictions for frequent prolonged hot temperature patterns, we aimed...
Hereford bulls are being used in dairy farming systems to sire calves that can be finished as beef cattle, bred cows would not otherwise for replacement cows. Their characteristically white faces offer simple markers parentage, however we observed some × crossbred have splotchy faces. We conducted a genome-wide association analysis face colour 128 Hereford-cross using genotype data from the GMK50kv1 chip and found adulteration of trait is caused by an epistatic interaction between mutations...
Abstract Animal health and welfare are at the forefront of public concern agricultural sector is responding by prioritising selection welfare-relevant traits in their breeding schemes. In some cases, welfare-enhancing such as horn-status (i.e., polled) or diluted coat colour, which could enhance heat tolerance, may not segregate breeds primary interest, highlighting gene-editing tools CRISPR-Cas9 technology an approach to rapidly introduce variation into these populations. A major limitation...