A5003729013- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Genomics and Chromatin Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
- RNA Interference and Gene Delivery
- Transgenic Plants and Applications
- Xenotransplantation and immune response
- Virus-based gene therapy research
- Infant Nutrition and Health
- Insect symbiosis and bacterial influences
- Chromosomal and Genetic Variations
- Genetically Modified Organisms Research
- Cancer-related gene regulation
- Glycosylation and Glycoproteins Research
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
AgResearch
2015-2025
University of Auckland
2020-2025
Maurice Wilkins Centre
2020-2022
Research Institute of Molecular Pathology
1997-2001
Vienna Biocenter
2000
Salk Institute for Biological Studies
1997
Institut de Biologie Structurale
1993
Université Grenoble Alpes
1993
Max Planck Institute for Molecular Genetics
1989-1993
University of Liège
1993
Journal Article Intercontinental Spread of a Multiresistant Clone Serotype 23F Streptococcus pneumoniae Get access Rosario Munoz, Munoz Search for other works by this author on: Oxford Academic PubMed Google Scholar Tracey J. Coffey, Coffey Margaret Daniels, Daniels Christopher G. Dowson, Dowson Gotz Laible, Laible Julio Casal, Casal Regine Hakenbeck, Hakenbeck Michael Jacobs, Jacobs James M. Musser, Musser Brian Spratt, Spratt ... Show more Alexander Tomasz The Infectious Diseases, Volume...
Higher-order chromatin has been implicated in epigenetic gene control and the functional organization of chromosomes. We have recently discovered mouse (Suv39h1) human (SUV39H1) histone H3 lysine 9-selective methyltransferases (Suv39h HMTases) shown that they modulate dynamics somatic cells. describe here isolation, chromosomal assignment, characterization a second murine gene, Suv39h2. Like Suv39h1, Suv39h2 encodes an HMTase shares 59% identity with Suv39h1 but which differs by presence...
Penicillin resistance in pneumococci is due to the appearance of high molecular-weight penicillin-binding proteins (PBPs) that have reduced affinity for antibiotic. We compared PBX 2x genes (pbpX) one penicillin-susceptible and five penicillin-resistant clinical isolates Streptococcus pneumoniae isolated from various parts world. All resistant contained a low-affinity form PBP 2x. The 2 kb region two differed at only eight nucleotide sites (0.4%) resulted single amino acid difference In...
SUV39H1, a human homologue of the Drosophila position effect variegation modifier Su(var)3-9 and Schizosaccharomyces pombe silencing factor clr4, encodes novel heterochromatic protein that transiently accumulates at centromeric positions during mitosis. Using detailed structure-function analysis SUV39H1 mutant proteins in transfected cells, we now show deregulated interferes multiple levels with mammalian higher-order chromatin organization. First, forced expression full-length (412 amino...
Summary Development of penicillin resistance in Streptococcus pneumoniae is due to successive mutations penicillin‐binding proteins (PBPs) which reduce their affinity for β‐lactam antibiotics. PBP2x one the high‐M r PBPs appears be altered both resistant clinical isolates, and cefotaxime‐resistant laboratory mutants. In this study, we have sequenced a 2564 base‐pair chromosomal fragment from penicillin‐sensitive S. strain R6, contains gene. Within fragment, 2250 open reading frame was found...
Milk from dairy cows contains the protein β-lactoglobulin (BLG), which is not present in human milk. As it a major milk allergen, we wished to decrease BLG levels by RNAi. In vitro screening of 10 microRNAs (miRNAs), either individually or tandem combinations, identified several that achieved as much 98% knockdown BLG. One construct was expressed mammary gland an ovine BLG-expressing mouse model, resulting 96% Following this vivo validation, produced transgenic calf, engineered express these...
Abstract Predictable, clean genetic modification (GM) in livestock is important for reliable phenotyping and biosafety. Here we reported the generation of isozygous, functional myostatin (MSTN) knockout cloned pigs free selectable marker gene (SMG) by CRISPR/Cas9 Cre/LoxP. CRISPR/Cas9-mediated homologous recombination (HR) was exploited to knock out (KO) one allele MSTN pig primary cells. Cre recombinase then used excise SMG with an efficiency 82.7%. The SMG-free non-EGFP cells were isolated...
We applied precise zygote-mediated genome editing to eliminate beta-lactoglobulin (BLG), a major allergen in cows' milk. To efficiently generate LGB knockout cows, biopsied embryos were screened transfer only appropriately modified embryos. Transfer of 13 pre-selected into surrogate cows resulted the birth three calves, one dying shortly after birth. Deep sequencing results confirmed conversion genotype from wild type edited nine bp deletion by more than 97% two male calves. The third calf,...
Penicillin-binding protein 2x (PBP 2x) of Streptococcus pneumoniae is one the high-molecular-weight PBPs involved in development intrinsic beta-lactam resistance. Point mutations PBP genes (pbpX) have now been characterized five independent spontaneous laboratory mutants order to identify regions which are important for interaction with antibiotics. All mutant contained two four resulting amino acid substitutions within penicillin-binding domain 2x, and none carried an identical set...
Correct reprogramming of epigenetic marks in the donor nuclei is crucial for successful cloning by nuclear transfer. Specific modifications, such as repressive histone lysine methylation marks, are known to be very stable and difficult reprogram. The discovery demethylases has opened up opportunities study effects removing cells prior In this study, we generated mouse embryonic stem (ES) inducible expression JMJD2B (also KDM4B), a demethylase that primarily removes histone-3 lysine-9...
Abstract The recent development of designer nucleases allows for the efficient and precise introduction genetic change into livestock genomes. Most studies so far have focused on random mutations in cultured cells use nuclear transfer to generate animals with edited genotypes. To circumvent intrinsic uncertainties inefficiencies we directed our efforts specific changes by homology-driven repair directly vitro produced embryos. Initially, injected zinc finger nuclease (ZFN)-encoding mRNA or...
Abstract Background High-producing Holstein Friesian dairy cattle have a characteristic black and white coat, often with large proportions of black. Compared to light coat color, absorbs more solar radiation which is contributing factor heat stress in cattle. To better adapt rapidly warming climates, we aimed lighten their color by genome editing. Results Using gRNA/Cas9-mediated editing, introduced three bp deletion the pre-melanosomal protein 17 gene ( PMEL ) proposed as causative variant...
Correct reprogramming of epigenetic marks is essential for somatic cells to regain pluripotency. Repressive histone (H) lysine (K) methylation are known be stable and difficult reprogram. In this study, we generated transgenic mice mouse embryonic fibroblasts (MEFs) the inducible expression KDM4B, a demethylase that removes H3 K9 H3K36 trimethylation (me3) (H3K9/36me3). Upon inducing Kdm4b, H3K9/36me3 levels significantly decreased compared non-induced controls. Concurrently, H3K9me1...
Transgenic mammals have been produced using sperm as vectors for exogenous DNA (sperm-mediated gene transfer (SMGT)) in combination with artificial insemination. Our study evaluated whether SMGT could also be achieved IVF to efficiently produce transgenic bovine embryos. We assessed binding and uptake of fluorescently labelled plasmids into the presence different concentrations dimethyl sulphoxide or lipofectamine. Live motile displayed a characteristic punctuate fluorescence pattern across...