A5044318757- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Renal and related cancers
- Viral Infectious Diseases and Gene Expression in Insects
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Virus-based gene therapy research
- Reproductive biology and impacts on aquatic species
- Reproductive Health and Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- Birth, Development, and Health
- RNA Interference and Gene Delivery
- Seed Germination and Physiology
- MicroRNA in disease regulation
- Food Allergy and Anaphylaxis Research
- Polyomavirus and related diseases
- Testicular diseases and treatments
- Full-Duplex Wireless Communications
- Allergic Rhinitis and Sensitization
- Anesthesia and Neurotoxicity Research
- Stress Responses and Cortisol
- Energy Harvesting in Wireless Networks
Islamic Azad University, Marvdasht
2012-2025
Islamic Azad University, Tehran
2025
Islamic Azad University of Shahrekord
2025
Islamic Azad University of Shiraz
2018-2022
Academic Center for Education, Culture and Research
2010-2016
Royan Institute
2009-2016
Islamic Azad University, Isfahan
2016
Islamic Azad University of Farahan
2015
Islamic Azad University, Science and Research Branch
2015
5-Aza-2′-deoxycytidine (AzC), trichostatin A (TSA), and its natural mimetic, sodium butyrate (NaB), are antineoplastic drugs that can modify the epigenetic status of donor cells prior to somatic cell nuclear transfer (SCNT). In this study, we used fibroblast treated with these investigate direct indirect effects induced changes in DNA methylation acetylation lysine 9 residue histone H3 (H3K9). Additionally, assayed cellular characteristics (cell growth, proliferation, cycle progression,...
The potential applications of a simplified method somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput considerable. Technically, major step SCNT to produce large pools enucleated oocytes (cytoplasts) efficiently, process requires considerable micromanipulation skill expensive equipment. Here, we have developed an efficient high-throughput manual oocyte enucleation using simple device, pulled Pasteur pipette, can be connected standard zona-free embryo...
The limited duration and compromised efficiency of oocyte-mediated reprogramming, which occurs during the early hours following somatic cell nuclear transfer (SCNT), may significantly interfere with epigenetic contributing to high incidence ill/fatal transcriptional phenotypes physiological anomalies occurring later pre- post-implantation events. A potent histone deacetylase inhibitor, trichostatin (TSA), was used understand effects assisted modifications on profiles SCNT blastocysts...
The purpose of this study was to develop an improved zona-free method goat somatic cell nuclear transfer (SCNT) that has both ease operation and efficiency. main steps involved were: (1) optimization in vitro oocyte maturation, (2) parthenogenetic activation oocytes, (3) SCNT anaphase II-telophase II (AII-TII) oocytes subverted the need for long term UV-exposure (4) culture groups cloned embryos wells a highly efficient continuous serum-free embryo medium blastocyst stage before recipients....
Chinese Hamster Ovary (CHO) cells are essential in biopharmaceutical manufacturing. Scientists use CRISPR to enhance productivity. mRNAs contain UTRs that regulate gene expression, affecting protein abundance. Targeting these regions creates desirable knockout cells. The Caspase 8 Associated Protein 2 (
<title>Abstract</title> <bold>Background and Purpose:</bold> Formononetin is a phytoestrogen isolated from several medicinal plants, it extensively investigated for its anti-cancer effects in cells. However, the impact of formononetin on hepatocellular carcinoma (HCC) has been unclear. In this study, influence cell growth, metabolism, signaling molecules examined HepG2 cells, singly combination with sorafenib. This study investigates individual combined sorafenib focusing miR-21 regulation...
ABSTRACT The viral haemorrhagic septicaemia virus ( VHSV ) is considered a very significant disease affecting fish. objective of this project to develop multi‐epitope vaccine targeting hemorrhagic fever using the reverse vaccinology approach. Epitope prediction was conducted immunogenic components VHSVV . A developed by combining proteins’ most potent B‐ and T‐cell epitopes with adjuvant. Following that, comprehensive evaluation carried out on many facets formulated vaccine, including its...
Background Egg yolk is the main cryoprotectant agent in semen freezing extenders which used order to protect spermatozoa against cold shock. However elimination of animal bioproducts from cryopreservation protocol becoming mandatory. Therefore aim this study compare a previously studied homemade soya bean lecithin based extender with commercially available for ram sperm cryopreservation. Materials and methods Samples three rams were pooled split into two equal aliquots diluted (1:20)...
Transgenic mammals have been produced using sperm as vectors for exogenous DNA (sperm-mediated gene transfer (SMGT)) in combination with artificial insemination. Our study evaluated whether SMGT could also be achieved IVF to efficiently produce transgenic bovine embryos. We assessed binding and uptake of fluorescently labelled plasmids into the presence different concentrations dimethyl sulphoxide or lipofectamine. Live motile displayed a characteristic punctuate fluorescence pattern across...
The effect of technical steps somatic cell nuclear transfer (SCNT) on different aspects cloned embryo development was investigated in sheep. In vitro-matured oocytes were enucleated the presence or absence zona and reconstituted by three SCNT techniques: conventional zona-intact (ZI-NT), standard zona-free (ZF-NT) intracytoplasmic injection (ICI-NT). Stepwise alterations remodeling events mRNA abundances, throughput efficiency allocation resulted blastocysts assessed. Early signs observed as...
In this study, fibroblast cells were stably transfected with mouse POU5F1 promoter-driven enhanced green fluorescent protein (EGFP) to investigate the effect of S-adenosylhomocysteine (SAH), reversible non-toxic inhibitor DNA-methyltransferases (DNMTs), at different intervals post-fusion on in vitro development cloned bovine embryos. Treatment SAH for 12 hr resulted 54.6 ± 7.7% blastocyst production, which was significantly greater than fertilized embryos (IVF: 37.2 2.7%), treated 72 (31.0...
Vitamin D (VD) deficiency reduces the chances of successful fertilization; however, it remains to be validated whether this effect is dependent or not on calcium. To address question, we generated several situation using a mouse model in which VD content was either increased decreased normo hypocalcemia context. After measurement serum 25-hydroxyvitamin D2, calcium and phosphorus levels, an analysis carried out terms oocytes maturation as well reproductive performance. overdose, despite fact...