A5067965301- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Spaceflight effects on biology
- Circadian rhythm and melatonin
- Mitochondrial Function and Pathology
- Evolution and Genetic Dynamics
- Genomics, phytochemicals, and oxidative stress
- Cellular Mechanics and Interactions
- Biocrusts and Microbial Ecology
- Rabbits: Nutrition, Reproduction, Health
- Photosynthetic Processes and Mechanisms
Institute for Research in Immunology and Cancer
2017-2025
Université de Montréal
2017-2022
Highlights•Mitotic spindles orient parallel to the tissue plane in C. elegans germ line•Spindle orientation is set up prophase and actively maintained through anaphase•Proper spindle depends upon LIN-5/NuMA dynein•Interphase centrosome position cortical dynamics predict orientationSummaryMitotic contributes organization shape by setting cell division plane. How coupled diverse architectures incompletely understood. The gonad a tube-shaped organ with cells forming circumferential monolayer...
The spindle assembly checkpoint (SAC) is a conserved mitotic regulator that preserves genome stability by monitoring kinetochore–microtubule attachments and blocking anaphase onset until chromosome biorientation achieved. Despite its central role in maintaining fidelity, the ability of SAC to delay exit presence attachment defects (SAC “strength”) appears vary widely. How different cellular aspects drive this variation remains largely unknown. Here we show strength correlated with cell fate...
The C. elegans germline is organized as a syncytium in which each germ cell possesses an intercellular bridge that maintained by stable actomyosin ring and connected to common pool of cytoplasm, termed the rachis. How cells undergo cytokinesis while maintaining this syncytial architecture not completely understood. Here, we use live imaging characterize primordial (PGC) division first-stage larvae. We show PGC connects it term proto-rachis. further first incomplete stabilized cytokinetic...
Investigating the complex interactions between stem cells and their native environment requires an efficient means to image them in situ. Caenorhabditis elegans germline (GSCs) are distinctly accessible for intravital imaging; however, long-term acquisition analysis of dividing GSCs can be technically challenging. Here we present a systematic investigation into technical factors impacting GSC physiology during live imaging provide optimized method monitoring mitosis under minimally...
Abstract Mitotic spindle orientation sets the cell division plane and is thus critical for maintaining tissue organization. The C. elegans gonad tube-shaped, with germ cells forming a circumferential monolayer around shared inner core of cytoplasm called rachis. Each connected to rachis via stable cytoplasmic bridge, polarizing along their rachis-basal axis. How this organization maintained during development unclear, as lack canonical cell-cell junctions that, in other types, ensure proper...
Plants of the Mimosa genus are studied and used for their bioactive properties. Among phytochemicals quercetin myricetin, which have been demonstrated to act as antioxidants in many contexts (Taheri et al. 2020; Xu 2019), including C. elegans (Buchter 2013; Grünz 2012; Sugawara Sakamoto 2020). Other from these plants, such triterpenoid phytosterol lupeol, shown antioxidant properties but not extensively characterized model organisms (Liu 2021; Shai 2009). Here we employed nematode assess...
Abstract The spindle assembly checkpoint (SAC) is a conserved mitotic regulator that preserves genome stability. Despite its central role in preserving the fidelity of mitosis, strength SAC varies widely between cell types. How adapted to different cellular contexts remains largely unknown. Here we show both size and fate impact strength. While smaller cells have stronger SAC, with germline increased activity relative their somatic counterparts across all sizes. We find enhanced blastomere P...
Abstract Investigating the complex interactions between stem cells and their native environment requires an efficient means to image them in situ . Caenorhabditis elegans germline (GSCs) are distinctly accessible for intravital imaging; however, long-term acquisition analysis of dividing GSCs can be technically challenging. Here we present a systematic investigation into technical factors impacting GSC physiology during live imaging provide optimized method monitoring mitosis under minimally...