Staphylococcus aureus is a gram-positive bacterium, commonly found in the nostrils, on skin and hair of warm-blooded animals, including humans. It can produce wide variety virulence factors, staphylococcal enterotoxins (SEs). In literature, 24 different SEs many variants have been identified; among these, only five (the so-called classic enterotoxins) well-defined. Due to their emetic activity, are frequently responsible for food poisoning, when consumers ingest contaminated food. proteins...

Zirconia (ZrO2) and alumina (Al2O3) based ceramics are widely used for load-bearing applications in bone repair due to their excellent mechanical properties biocompatibility. They often regarded as bioinert since no direct bone–material interface is created unless a porous structure intercedes, leading better bonding. In this regard, investigating interactions between cells of great interest. the present study, we report on successful fabrication sintered A-61, zirconia Z-50 zirconia/alumina...

Metal-bisphosphonates are self-sacrificial controlled delivery systems that achieve ultra-long release of anti-osteoporotic bisphosphonate drugs.

Functionalized calcium phosphate nanoparticles with osteogenic activity were prepared. Polyethyleneimine-stabilized coated a shell of silica and covalently functionalized by silanization thiol groups. Between the surface outer shell, plasmid DNA which encoded either for bone morphogenetic protein 7 (BMP-7) or enhanced green fluorescent was incorporated as cargo. The DNA-loaded used transfection pre-osteoblastic MC3T3-E1 cells. cationic showed high efficiency together low cytotoxicity. Their...

It is acknowledged that cellular responses are highly affected by biomaterial porosity. The investigation of this effect important for the development implanted biomaterials integrate with bone tissue. Zirconia and alumina ceramics exhibit outstanding mechanical properties among most popular implant materials used in orthopedics, but few data exist regarding porosity on to these materials. present study investigates attachment proliferation pre-osteoblastic cells zirconia alumina. For each...

The CC chemokine eotaxin-1 (CCL11) is chemotactic for eosinophils, basophils, and type 2 helper T cells may play a role in allergic inflammation. We investigated its contribution as an eosinophil chemoattractant asthmatic airway secretions (sampled induced sputum), which possess activity eosinophils cells. Sputum samples collected from healthy subjects with mild, stable-moderate, unstable-moderate, severe asthma were processed phosphate-buffered saline assayed eotaxin by ELISA...

Dense zirconia ceramics are used in bone applications due to their mechanical strength and biocompatibility, but lack osseointegration. A porous interface contact with tissue may lead better bonding the biological properties of not widely explored. The present study focuses on manufacturing an yttria- (YSZ) a magnesia-stabilized (MgSZ) zirconia, vitro investigation. sintered had similar characteristics porosity, pore size interconnectivity. Their elastic moduli compressive values were within...

Mammalian α-defensins all have a conserved triple-stranded β-sheet structure that is constrained by an invariant tridisulfide array, and the peptides exert bactericidal effects permeabilizing target cell envelope. Curiously, disordered, disulfide-null variant of mouse α-defensin cryptdin-4 (Crp4), termed (6C/A)-Crp4, has activity equal to or greater than native peptide, providing rationale for comparing mechanisms which interact with disrupt phospholipid vesicles defined composition. For...

ABSTRACT: Pleurocidin, an antimicrobial peptide originally isolated from the winter flounder (Pleuronecetes americanus), was evaluated for potential use in food application. Eighteen microbial species, including yeasts, molds, and foodborne bacterial pathogens, were evaluated. Liquid‐based minimal inhibitory concentration (MIC) technique used to test activity of synthetic peptide. Hemolytic cytotoxic effects on human cells investigated using colorimetric analysis. Pleurocidin found be...

Ready to Eat (RTE) cooked meat products are among the most consumed RTE food subcategories in EU.They also associated with highest number of listeriosis cases per year.Contamination Listeria monocytogenes may arise from post-processing and its growth is often supported by pH water activity product.L. grow during refrigeration reach unacceptable levels at time consumption, posing a public health risk.The aim this study was conduct Quantitative Microbiological Risk Assessment (QMRA) L....

Ground beef contamination with Escherichia coli is usually a result of carcass faecal during the slaughter process. Carcasses are contaminated when they come into contact soiled hides or intestinal leakage content dressing and evisceration processes. A more recent compelling hypothesis that, lymph nodes present in manufacturing trimmings, can be potential source Enterobacteriaceae ground beef. The aim this study was to investigate occurrence E. from carcasses used for meat production, six...

Abstract Objectives Non-steroidal anti-inflammatory drugs (NSAIDs), used in the treatment of musculoskeletal pathologies, have been associated with impaired bone healing, possibly through inhibition osteogenic differentiation. The adipose tissue (AT) is regarded as an attractive source stromal cells for autologous cell transplantation bone. effects NSAIDs on human AT-derived (hADSCs) are unknown. Methods We examined effect several including meloxicam, parecoxib, lornoxicam, diclofenac and...

Non‑steroidal anti‑inflammatory drugs (NSAIDs) are widely used in the treatment of a variety musculoskeletal conditions, injuries and after surgery for postoperative pain management. Their use has been associated with impaired bone healing, possibly due to multifactorial function, which may include inhibition osteoblast recruitment differentiation. However, up date, there is no consensus regarding impact NSAIDs on bone‑healing. The aim current study was investigate effects five cellular...

Bisphosphonate (BP)-based treatments have been extensively prescribed for bone-related conditions, particularly osteoporosis. Their low bioavailability creates the need dosage increase to reach therapeutic levels but generates a plethora of undesirable side effects. A viable approach alleviating these issues is design and exploit controlled release strategies. Herein, profiles 15 structurally characterized BPs (actual drugs structural analogs) were thoroughly studied from tablets containing...

The aim of this study was to compare the effect a commercial formulation consisting natural ingredients and dextrose on production salami. We analysed microbiological, physico-chemical sensorial properties in nitrite-free swine roe deer (Capreolus capreolus) Six batches salami were prepared: four with addition starter cultures diverse substrates (no substrate, skimmed milk, dextrose) two without as controls (one substrate one milk). Samples for microbiological analysis taken day 0, 5 25. A...