A5003399387- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Electrowetting and Microfluidic Technologies
- Biosensors and Analytical Detection
- Chemical Synthesis and Analysis
- 3D Printing in Biomedical Research
- Bacteriophages and microbial interactions
- Scientific Computing and Data Management
- Image Processing Techniques and Applications
- Marine and coastal ecosystems
- Melamine detection and toxicity
- Cell Image Analysis Techniques
- Electrohydrodynamics and Fluid Dynamics
- Microbial Community Ecology and Physiology
- Microbial Metabolic Engineering and Bioproduction
- Protist diversity and phylogeny
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2018-2024
Friedrich Schiller University Jena
2018-2021
Chungbuk National University
2015
High-throughput microbiological experimentation using droplet microfluidics is limited due to the complexity and restricted versatility of available detection techniques. Current setups are bulky, complicated, expensive, require tedious optical alignment procedures while still mostly fluorescence. In this work, we demonstrate an optofluidic setup for multi-parametric analyses samples by easily integrating micro-lenses embedding fibers guiding light in out microfluidic chip. The was validated...
Abstract The candidate phyla radiation (CPR) represents a distinct monophyletic clade and constitutes major portion of the tree life. Extensive efforts have focused on deciphering functional diversity its members, primarily using sequencing-based techniques. However, cultivation success remains scarce, presenting significant challenge, particularly in CPR-dominated groundwater microbiomes characterized by low biomass. Here, we employ an advanced high-throughput droplet microfluidics...
In order to leverage the immense potential of droplet microfluidics, it is necessary simplify process chip design and fabrication. While polydimethylsiloxane (PDMS) replica molding has greatly revolutionized chip-production process, its dependence on 2D-limited photolithography restricted possibilities, as well further dissemination microfluidics non-specialized labs. To break free from these restrictions while keeping fabrication straighforward, we introduce an approach produce complex...
High-speed multiwavelength fluorescence measurements are of paramount importance in microfluidic analytics. However, multicolor detection requires an intricate arrangement multiple detectors and meticulously aligned filters dichroic beamsplitters that counteract the simplicity, versatility, low cost approaches. To break free from restrictions optical setup complexity, we introduce a simpler single-sensor based on laser-frequency modulation frequency-division multiplexing (FDM). We modulate...
Abstract Nonribosomal peptide synthetases (NRPSs) are giant enzymatic assembly lines that deliver many pharmaceutically valuable natural products, including antibiotics. As the search for new antibiotics motivates attempts to redesign nonribosomal metabolic pathways, more robust and rapid sorting screening platforms needed. Here, we establish a microfluidic platform reliably detects production of model gramicidin S. The detection is based on calcein-filled sensor liposomes yielding increased...
Microfluidic emulsion-based droplet systems have a great potential for inexpensive ultrahigh-throughput experimentation. Yet, picking and upscaling single unique picoliter-sized droplets of interest out million others deeper analysis is still fundamental limitation. In order to overcome this missing gap, we present system in which are collected into collection chamber (DropLot) high throughput then slowly redirected an agar surface or microtiter plate via capillary tube passing through...
Droplet microfluidics has demonstrated immense potential in microbiological studies due to its unique features, such as miniaturization, compartmentalization, and parallelization. Multiplexing droplet content allows the investigation of various experimental conditions a highly parallelized manner. Yet, library generation tracking remain challenging high-throughput screening. The introduction distinct reagents into droplets necessitates precise control over flow microfluidic chip, limiting...
Abstract Nonribosomal peptide synthetases (NRPSs) are giant enzymatic assembly lines that deliver many pharmaceutically valuable natural products, including antibiotics. As the search for new antibiotics motivates attempts to redesign nonribosomal metabolic pathways, more robust and rapid sorting screening platforms needed. Here, we establish a microfluidic platform reliably detects production of model gramicidin S. The detection is based on calcein‐filled sensor liposomes yielding increased...
Droplet based microfluidic technology is a miniaturized platform for microbial analysis on picoliter scale. With its costefficiency, high-throughput and feasibility of complex handling protocols, droplet microfluidics favorable applications such as microorganism screening or synthetic biology. Scattered-light-based detection, in comparison to the widely used fluorescent-label-based approach, provides contact-free label-free, yet sensitive measuring solution. The angular dependency scattered...
Abstract In recent years, microfluidic technologies were introduced for massively parallel cultivation and screening approaches. Individual cells can easily be singularized, compartmentalized, cultivated from mixed inocula using droplet microfluidics. The generation of millions droplets in a high-throughput manner enables studying diverse samples combining the evaluation genetic phenotypic variants. It is powerful tool to explore exploit natural metabolic diversity.