A5045768401- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Polymer Surface Interaction Studies
- Photonic Crystals and Applications
- thermodynamics and calorimetric analyses
- Cell Image Analysis Techniques
- Characterization and Applications of Magnetic Nanoparticles
- Mechanical and Optical Resonators
- Metallic Glasses and Amorphous Alloys
- Metal and Thin Film Mechanics
- Magnetic properties of thin films
- Cell Adhesion Molecules Research
- Photonic and Optical Devices
- Theoretical and Computational Physics
- Near-Field Optical Microscopy
- Magnetic Properties and Applications
- Advanced Materials Characterization Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Thermodynamic and Structural Properties of Metals and Alloys
- Advanced Electron Microscopy Techniques and Applications
- Diamond and Carbon-based Materials Research
- Plasmonic and Surface Plasmon Research
Moscow Engineering Physics Institute
2020-2024
Sarov Institute of Physics and Technology
2020-2024
Tufts University
2013-2022
Medford Radiology Group
2019-2020
Clarkson University
2009-2013
Lomonosov Moscow State University
2004-2013
Toyohashi University of Technology
2008-2011
Chungnam National University
2004
The modulus of elasticity soft materials on the nanoscale is interest when studying thin films, nanocomposites, and biomaterials. Two novel modes atomic force microscopy (AFM) have been introduced recently: HarmoniX PeakForce QNM. Both produce distribution maps elastic over sample surface. Here we investigate question how quantitative these are materials. Three different polymers with a macroscopic Young's 0.6–0.7 GPa (polyurethanes) 2.7 (polystyrene) analyzed using new modes. moduli...
It is of interest to measure the modulus rigidity at small indentation depths for many systems, such as thin films, nanocomposites, biomaterials, etc. Depth-dependence homogeneous soft materials broadly observed in nanoindentation experiments. Typically, reaches its bulk value only when depth becomes relatively large. Nature this effect (we suggest call "skin-effect" short) not well understood. even clear if a real or an artifact. Here we present results precise measurements based on use...
Significance New noninvasive and accurate diagnostic tests of cancer are important. Here we describe such a test, which is applied to the detection bladder cancer, one most common cancers cause cancer-related deaths. This method can also be for other cancers, in cells or body fluid available analysis without need invasive biopsy, e.g., upper urinary tract, urethra, colorectal gastrointestinal, cervical, aerodigestive etc. Furthermore, described approach extended detect cell abnormalities...
Here we show that the surface of human cervical epithelial cells demonstrates substantially different fractal behavior when cell becomes cancerous. Analyzing adhesion maps individual cells, which were obtained using atomic force microscopy operating in HarmoniX mode, found cancerous demonstrate simple behavior, whereas normal can only be approximated at best as multifractal. Tested on ~300 collected from 12 humans, dimensionality is to unambiguously higher than for cells.
Despite considerable advances in understanding the molecular nature of cancer, many biophysical aspects malignant development are still unclear. Here we study physical alterations surface human cervical epithelial cells during stepwise vitro cancer (from normal to immortal (premalignant), malignant). We use atomic force microscopy demonstrate that is associated with emergence simple fractal geometry on cell surface. Contrary previously expected correlation between and fractals, find occurs...
Abstract Dynamic mechanical spectroscopy (DMS), which allows measuring frequency-dependent viscoelastic properties, is important to study soft materials, tissues, biomaterials, polymers. However, the existing DMS techniques (nanoindentation) have limited resolution when used on preventing them from being mechanics at nanoscale. The nanoindenters are not capable of cells, nanointerfaces composite materials. Here we present a highly accurate modality, combination three different methods:...
At present, a technique potentially capable of measuring values Young's modulus at the nanoscale is atomic force microscopy (AFM) working in indentation mode. However, question if AFM data can be translated into absolute not well-studied as yet, particular, for most interesting case stiff nanocomposite materials. Here we investigate this question. A special sample material, shale rock, was used, which relatively homogeneous multi-micron scale. Two modes, force-volume and PeakForce QNM were...
The present study aims at integrating drug-releasing materials with signal-processing biocomputing systems. Enzymes alanine transaminase (ALT) and aspartate (AST)---biomarkers for liver injury---were logically processed by a biocatalytic cascade realizing Boolean AND gate. Citrate produced in the system was used to trigger drug-mimicking release from alginate microspheres. In order differentiate low vs. high concentration signals, microspheres were coated protective shell composed of...
Abstract Recently developed sub-resonance tapping modes (such as Digital Pulse, Peak Force Tapping, HybriD, etc.) of atomic force microscopy (AFM) allow imaging compositional contrast (bio)materials and biological cells down to the nanoscale. Here we report on a powerful extension those modes, “ringing” mode, which more than doubles number non-trivial physical channels that can be collected with regular tapping. It simultaneously record five new additional parameters related adhesive...
When studying the mechanical properties of cells by an indentation technique, it is important to take into account nontrivial pericellular interface (or "brush") which includes a coating and corrugation membrane (microvilli microridges). Here we use atomic force microscopy (AFM) study mechanics cortical neurons taking presence above brush surrounding cell soma. We perform systematic both layer underlying neuron soma demonstrate that likely responsible for low elastic modulus (<1 kPa)...
A novel method based on atomic force microscopy (AFM) working in Ringing mode (RM) to distinguish between two similar human colon epithelial cancer cell lines that exhibit different degrees of neoplastic aggressiveness is reported on. The classification accuracy identifying the line images a single can be as high 94% (the area under receiver operating characteristic [ROC] curve 0.99). Comparing using RM and regular imaging channels, it seen channels are responsible for accuracy. cells also...
In this study, we examine the topography and adhesion images of cell surface neutrophils during activation process. Our analysis parameters indicates that most significant changes in occur within first 30 min activation, suggesting reactive oxygen species may require approximately amount time to activate cells. Interestingly, observed granular structure as early 10 after neutrophil when examining atomic force microscopy images. This finding aligns with reorganization cells under confocal...
Polymers brushes (polymer chains end-tethered to a substrate) have been extensively studied with atomic force microscopy (AFM). Force–indentation curves are collected while squeezing the sample surface AFM probe. The analysis of these allows obtaining equilibrium brush thickness and grafting density by using an appropriate model for mechanical deformation brush. However, this approach becomes inaccurate when substrate is deformable, which frequently case. In situation, force–distance include...
New ultrabright fluorescent silica nanoparticles for targeting cancers <italic>in vivo</italic> are presented.
Experimental and theoretical studies of light coupling to various magnetic nanostructured media nanocomposites are briefly reported. Enhancement the magneto-optical response is shown occur when constitutive materials photonic crystals magnetic. Transmission reflection types 1D magnetophotonic (MPCs) have been studied. New possibility enhance has found utilizing localized surface plasmon resonances in bismuth-substituted yttrium iron garnet (Bi:YIG) films impregnated with Au nanoparticles....
Here we describe a non-traditional method to identify cancerous human cervical epithelial cells in culture dish based on physical adhesion between silica beads and cells. It is simple optical fluorescence-based technique which detects the relative difference amount of fluorescent physically adherent surfaces normal The utilizes centripetal force gradient that occurs rotating dish. Due variation balance forces, demonstrate clearly distinctive distributions particles cell surface over...
Here we report on the first ultrabright fluorescent nanothermometers, ∼50 nm-size particles, capable of measuring temperature in 3D and down to nanoscale. The is measured through recording ratio fluorescence intensities dyes encapsulated inside nanochannels silica matrix each nanothermometer. brightness particle excited at 488 nm equivalent coming from 150 molecules rhodamine 6G 1700 B dyes. both with a single wavelength due Förster resonance energy transfer (FRET). We demonstrate repeatable...