A5081096761- Phytoestrogen effects and research
- Monoclonal and Polyclonal Antibodies Research
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Glycosylation and Glycoproteins Research
- Ginseng Biological Effects and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Bioactive Compounds and Antitumor Agents
- Phytochemistry and Biological Activities
- Protein purification and stability
- Probiotics and Fermented Foods
- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- Phytochemical compounds biological activities
- Protein Hydrolysis and Bioactive Peptides
- Phytochemistry and biological activity of medicinal plants
- Flavonoids in Medical Research
- Cholesterol and Lipid Metabolism
- Chromatography in Natural Products
- Plant tissue culture and regeneration
- Biosensors and Analytical Detection
- Phytochemistry and Bioactivity Studies
- Phytochemical Studies and Bioactivities
- Advanced Biosensing Techniques and Applications
- Estrogen and related hormone effects
Kyushu University
2015-2024
Kumamoto University
2018-2021
Fukuoka University
2008-2018
Pharmac
2008-2017
Nagasaki International University
2012-2013
Aichi Cancer Center
1989
The peptide linker between variable domains of heavy (VH) and light (VL) chains is one important factors that influence the characteristics scFv, including binding activity specificity against target antigen. scFvs daidzin (DZ-scFvs) with different lengths were constructed in format VH-(GGGGS)n-VL (n = 1, 3, 5, 7). They expressed hemolymph silkworm larvae using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA system, their reactivity related compounds evaluated an indirect competitive...
This study outlines a green process for Centella asiatica (L.) Urb. (CA) extraction. Natural deep eutectic solvents (NADESs) and microwave-assisted extraction (MAE) were combined to provide high bioactive compound yield antioxidant activity. Among the NADESs evaluated, combination of acetylcholine chloride : malic acid water (1 2 2): (40 60) was best These conditions madecassoside (MS) (21.7 mg g-1 dry weight) asiaticoside (AS) (12.7 yields, with greater than 80% (v/v) EtOH (13.3 MS 7.80...
Licochalcone A (LicoA), which is found in the root of Chinese licorice (Glycyrrhiza inflata Batalin), has been reported as an effective anti-microbial and anti-inflammation was approved for treatment rosacea acne clinical therapeutic. To develop a monoclonal antibody against LicoA, mannich reaction hapten conjugate used immunization, followed by hybridoma technique. Enzyme-linked immunosorbent assay (ELISA) quantitative determination LicoA developed using constructed antibody. The validation...
We constructed a single-chain variable fragment (scFv) antibody against plumbagin (PL) with improved specific binding to PL. Variable heavy- and light-chain genes were cloned directly from the cDNA of hybridoma cell line 3A3 assembled using splice-overlap extension polymerase chain reaction (SOE-PCR) primers including flexible peptide (Gly4Ser)3 linker primers. The scFv gene was ligated into pET28a expression vector transformed Escherichia coli BL21 (DE3). denatured protein expressed as...
Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein, and genistein as major isoflavonoids used for its rejuvenating estrogenic effects. In order to analyze these compounds, single enzyme-linked immunosorbent assay (ELISA) total was developed using anti-puerarin anti-daidzin polyclonal antibodies (PAbs). The range calibration of by ELISA 0.05–6.25 µg/mL. Total isoflavonoid concentrations in P. samples determined the newly system showed...
Influence of elicitors on plumbagin accumulation in Drosera indica whole plant culture was investigated. Treatment plantlets with 150 mg/l chitosan and 100 μM salicylic acid mostly stimulated production after 3 days elicitation, while 0.5 mg/ml yeast extract 50 methyl jasmonate elicited at 6 days. Yeast (0.5 mg/ml) the most efficient elicitor to enhance (2.69±0.03 mg/g dry wt.) by 5.4-fold over control (0.50±0.01 wt.). Present results indicated effectiveness elicitation in in vitro culture...