A5100637376- Glycosylation and Glycoproteins Research
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Microbial Natural Products and Biosynthesis
- Peptidase Inhibition and Analysis
- Biochemical Analysis and Sensing Techniques
- Software-Defined Networks and 5G
- Geographic Information Systems Studies
- Thyroid Cancer Diagnosis and Treatment
- Semantic Web and Ontologies
- Galectins and Cancer Biology
- RNA modifications and cancer
- Synthetic Organic Chemistry Methods
- Folate and B Vitamins Research
- Natural Compounds in Disease Treatment
- Heat transfer and supercritical fluids
- Receptor Mechanisms and Signaling
- Network Traffic and Congestion Control
- Autophagy in Disease and Therapy
- Natural product bioactivities and synthesis
- Marine Sponges and Natural Products
- Sirtuins and Resveratrol in Medicine
Center for Excellence in Molecular Plant Sciences
2016-2024
Chinese Academy of Sciences
2016-2024
Shanxi Medical University
2024
Scripps Research Institute
2020-2024
First Hospital of Shanxi Medical University
2024
Northeast Electric Power University
2023-2024
Kunming University of Science and Technology
2019-2023
First People's Hospital of Yunnan Province
2021-2023
Aerospace Information Research Institute
2021-2023
Qilu University of Technology
2023
Significance The development of selective bioactive compounds that target RNA structures is difficult. Here we report an approach to identify low molecular weight fragments bind a cancer-causing RNA. By determining where the within target, they can be assembled provide high-affinity and potent inhibitor. This could general way develop small molecules RNA, which previously was considered “undruggable.” It applied precision lead medicine against contributes cancer metastasis.
The chemical proteomic properties of five diazirine-based, fully-functionalized photoaffinity tags, including a newly developed, minimal tag, were compared. This study provides guidance for the development new probes.
Natural disasters have frequently occurred and caused great harm. Although the remote sensing technology can effectively provide disaster data, it still needs to consider relevant information from multiple aspects for analysis. It is hard build an analysis model that integrate large-scale information, particularly at sematic level. This paper proposes a prediction knowledge graph by integrating geographic with expert in field of constructs conceptual layer instance building common semantic...
The antimalarial drug artemisinin is found to have diverse biological activities ranging from anti-inflammatory anticancer properties; however, as of today, the cellular targets and mechanism action this important compound remained elusive. Here, we report global protein target profiling in HeLa cancer cell proteome using a chemical proteomics approach. In presence hemin, multiple proteins were targeted by probe through covalent modification. Further studies revealed that reducing hemin heme...
Significance Because of the weak interactions between individual glycan-binding proteins (GBPs), such as galectin-3, and glycans, strategies that enable direct interrogation these in living cells remain limited. Thus, glycan glycoprotein ligands are physiologically relevant for galectin-3 binding insufficiently described. Here we used a proximity labeling approach catalytically tags interactors identified its pertinent counter-receptors live cells. This study demonstrates is powerful tool...
Forest fires have frequently occurred and caused great harm to people’s lives. Many researchers use machine learning techniques predict forest by considering spatio-temporal data features. However, it is difficult efficiently obtain the features from large-scale, multi-source, heterogeneous data. There a lack of method that can effectively extract required learning-based fire predictions multi-source This paper proposes prediction integrates knowledge graphs models. fuse constructing...
Colorectal cancer remains the second leading cause of cancer-related mortalities worldwide. While artemisinin (ART), a key active compound from traditional Chinese medicinal herb Artemisia annua, has been recognized for its antiproliferative activity against colon cells, underlying molecular underpinnings remain elusive. Whereas promiscuity heme-dependent alkylating macromolecules, mainly proteins, seen pivotal as universal and primary mode action ART in accumulating evidence suggests...
We reported the proteome-wide profiling of cellular targets celastrol in HeLa cells<italic>via</italic>competitive chemoproteomics approach utilizing a cysteine-targeting activity-based probe.
Abstract Rifamycin-derived drugs, including rifampin, rifabutin, rifapentine, and rifaximin, have long been used as first-line therapies for the treatment of tuberculosis other deadly infections. However, late steps leading to biosynthesis industrially important rifamycin SV B remain largely unknown. Here, we characterize a network reactions underlying SV, S, L, O, B. The two-subunit transketolase Rif15 cytochrome P450 enzyme Rif16 are found mediate, respectively, unique C–O bond formation...
Functional discovery and characterization of the target enzymes responsible for biosynthesis pathway coded genes is ongoing, unknown functional diversity this class has been revealed by genome sequencing. Commonly, it feasible in annotating biosynthetic prokaryotes due to existence gene clusters secondary metabolites. However, eukaryotes, are not compactly clustered way prokaryotes. Hence, remains challenging identify pathways newly discovered natural products plants. Steviol glycosides one...
Abstract Folates are indispensable for plant development, but their molecular mode of action remains elusive. We synthesized a probe, “5-F-THF-Dayne,” comprising 5-formyl-tetrahydrofolate (THF) coupled to photoaffinity tag. Exploiting this probe in an affinity proteomics study Arabidopsis thaliana, we retrieved 51 hits. Thirty interactions were independently validated with vitro expressed proteins bind 5-F-THF high or low affinity. Interestingly, the interactors reveal associations beyond...
Background and objectivesThe purpose of this research was to develop validate the first prognostic nomograms for 3-, 5-, 10-year cancer-specific survival (CSS) overall (OS) in patients diagnosed with locally advanced thyroid cancer (LATC) by evaluating independent predictors prognosis a population LATC patients.MethodsDemographics, clinicopathologic characteristics, treatment, follow-up 2396 surveillance, epidemiology, end results database from 2004 2015 were retrospectively analyzed...
Despite the fact that multiple artemisinin-alkylated proteins in Plasmodium falciparum have been identified recent studies, alkylation mechanism and accurate binding site of artemisinin–protein interaction remained elusive. Here, we report chemical-probe-based enrichment artemisinin-binding peptide characterization P. translationally controlled tumor protein (TCTP). A fragment within N-terminal region TCTP was enriched found to be alkylated by an artemisinin-derived probe. MS2 fragments...
Spinal cord injury (SCI) is a neurological disease associated with high disability rate. Low-dose lipopolysaccharide (LPS) has been reported to activate cross-immune tolerance and alleviate the effects of various traumatic stimuli. The present study aimed explore effect LPS on SCI potential molecular mechanism.Male Sprague-Dawley (SD) rats were used established an in vivo model intraperitoneally injected lentivirus particles encoding MALAT1 small interfering RNA (siRNA) day 10 prior 0.2...
Pseudolaric acid B targets CD147 in human cancer cells.