A5017895796- Surfactants and Colloidal Systems
- Extracellular vesicles in disease
- Electrostatics and Colloid Interactions
- Field-Flow Fractionation Techniques
- Lipid metabolism and biosynthesis
- Hydrogels: synthesis, properties, applications
- MicroRNA in disease regulation
- Lipid Membrane Structure and Behavior
- Particle Dynamics in Fluid Flows
- Advanced Polymer Synthesis and Characterization
- Polymer Surface Interaction Studies
- Various Chemistry Research Topics
- Material Dynamics and Properties
- Spectroscopy and Chemometric Analyses
- Polymer crystallization and properties
- RNA Interference and Gene Delivery
- Cyclone Separators and Fluid Dynamics
- Analytical Chemistry and Chromatography
- Blood properties and coagulation
- Spectroscopy Techniques in Biomedical and Chemical Research
- Microplastics and Plastic Pollution
- Lattice Boltzmann Simulation Studies
- biodegradable polymer synthesis and properties
- Plant tissue culture and regeneration
- Microbial Inactivation Methods
National Institute of Chemistry
2015-2020
University of Ljubljana
2012-2019
In the past few years extracellular vesicles called exosomes have gained huge interest of scientific community since they show a great potential for human diagnostic and therapeutic applications. However, an ongoing challenge is accurate size characterization quantification because lack reliable techniques. this work, emphasis was focused on method development to size-separate, characterize, quantify small amounts by asymmetrical-flow field-flow fractionation (AF4) technique coupled...
Abstract Human plasma is a complex fluid, increasingly used for extracellular vesicle (EV) biomarker studies. Our aim was to find simple EV-enrichment method reliable quantification of EVs in be as disease. Plasma ten healthy subjects processed using sedimentation rate- (sucrose cushion ultracentrifugation—sUC) and size- (size exclusion chromatography—SEC) based methods. According nanoparticle tracking analysis (NTA), asymmetrical flow field-flow fractionation coupled detectors...
A fast, robust, and convenient method for quantitative determination of polyamide-6 (PA6) polyamide-66 (PA66) in plastic wastes is presented. The includes a straightforward procedure complete hydrolysis polyamides (PAs) into the constituent monomers their hydrolyzates by high-performance liquid chromatography on mixed-mode column, from which contents particular PA are determined. was developed neat PA6 PA66 as well mixture further utilized PA-based composites postconsumer (carpet fishing net...
The neurotropic and extremophilic black yeast Exophiala dermatitidis (Herpotrichellaceae) inhabits diverse indoor environments, in particular bathrooms, steam baths, dishwashers. Here, we show that the selected strain, EXF-10123, is polymorphic, can grow at 37 °C, able to assimilate aromatic hydrocarbons (toluene, mineral oil, n-hexadecane), shows abundant growth with neurotransmitters (acetylcholine, gamma-aminobutyric acid, glycine, glutamate, dopamine) as sole carbon sources. We have for...
An asymmetrical flow field-flow fractionation (AF4) technique coupled to a multiangle light scattering (MALS) detector with an embedded dynamic (DLS) module was introduced study the size characteristics and shape of soft particles various type: polystyrene nanosphere standards, lipid droplets (LDs), large unilamellar vesicles (LUVs). A range velocities through LS detector, at which accurate hydrodynamic can be extracted from DLS in mode, studied since subjected longitudinal exhibit not only...
Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes compositions of harvested missing. A plausible model description EV isolates has not been developed. Furthermore, identity genesis EVs still obscure relevant parameters have yet identified. The purpose this work is to better understand mechanisms taking place during harvesting EVs,...
Mixtures of polyelectrolytes and oppositely charged surfactants show very rich phase behavior that is influenced by surfactant–ion polyion properties water content. A general feature associative separation as a result strong electrostatic interactions, whereas the effect eventual more specific interactions (e.g., hydrophobic) has not been thoroughly investigated. In this paper, we report detailed study on structures in poly(styrenesulfonate anion) (PSS–)–cetyltrimethylammonium cation...
We study the structure of aggregates formed by isotactic and atactic poly(methacrylic acid)s in aqueous solutions with added alkali chlorides using light scattering.
Understanding of the interactions between proteins and natural artificially prepared lipid membrane surfaces embedded nonpolar cores is important in studies physiological processes their pathologies applicable to nanotechnologies. In particular, rapidly growing interest cellular droplets defines need for simplified biomimetic model systems overcome vivo complexity variability. We present a protocol preparation kinetically stable nanoemulsions with nanodroplets composed sphingomyelin (SM)...