A5001039341- Estrogen and related hormone effects
- Nuclear Physics and Applications
- Atomic and Subatomic Physics Research
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- HER2/EGFR in Cancer Research
- Computational Drug Discovery Methods
- Radiation Detection and Scintillator Technologies
- Quantum, superfluid, helium dynamics
- Receptor Mechanisms and Signaling
- Steroid Chemistry and Biochemistry
- DNA and Nucleic Acid Chemistry
- Bioactive Compounds and Antitumor Agents
- Chemical Synthesis and Analysis
- Retinoids in leukemia and cellular processes
- Nuclear physics research studies
- Nuclear reactor physics and engineering
- Advanced Breast Cancer Therapies
- Inflammatory mediators and NSAID effects
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Reproductive System and Pregnancy
- Particle physics theoretical and experimental studies
- Cancer-related molecular mechanisms research
- Breast Cancer Treatment Studies
Oak Ridge National Laboratory
2011-2024
University of Tennessee at Knoxville
2015-2024
University of Chicago
2015-2024
College of Wooster
2024
Tulane University
2023
Loyola University Chicago
2022
University of Illinois Chicago
1991-2021
The University of Texas MD Anderson Cancer Center
2018-2020
Baylor College of Medicine
1986-2020
Dan L Duncan Comprehensive Cancer Center
2020
The mechanism by which the estrogen receptor and other steroid hormone receptors regulate gene expression in eukaryotic cells is not well understood. In this study, a complementary DNA clone containing entire translated portion of messenger RNA for from MCF-7 human breast cancer was sequenced then expressed Chinese hamster ovary (CHO-K1) to give functional protein. An open reading frame 1785 nucleotides corresponded polypeptide 595 amino acids molecular weight 66,200, good agreement with...
Abstract The existence of a putative membrane estrogen receptor (ER) has been supported by studies accomplished over the past 20 yr. However, origin and functions this are not well defined. To study receptor, we transiently transfected cDNAs for ERα or ERβ into Chinese hamster ovary (CHO) cells. Transfection resulted in single transcript Northern blot, specific binding labeled 17β-estradiol (E2), expression ER both nuclear cell fractions. Competitive compartments revealed near identical...
High specific activity estradiol labeled with iodine-125 was used to detect approximately 200 saturable, high-affinity (dissociation constant ≅ 1.0 n M ) nuclear binding sites in rat (ROS 17/2.8) and human (HOS TE85) clonal osteoblast-like osteosarcoma cells. Of the steroids tested, only testosterone exhibited significant cross-reactivity estrogen binding. RNA blot analysis a complementary DNA probe receptor revealed putative transcripts of 6 6.2 kilobases both Type I procollagen...
Poly(A)+ RNA isolated from the human breast cancer cell line MCF-7 was fractionated by sucrose gradient centrifugation and fractions enriched in estrogen receptor (ER) mRNA were used to prepare randomly primed cDNA libraries lambda gt10 gt11 vectors. Clones corresponding ER sequence both after screening with either monoclonal antibodies (lambda gt11) or synthetic oligonucleotide probes designed two peptide sequences of purified gt10). Five clones antibody five oligonucleotides. The largest...
Estrogen receptors (ER) and progesterone (PgR) were studied immunohistochemically using specific antireceptor monoclonal antibodies in uterine tissue samples from 33 women various stages of the menstrual cycle. Immunohistochemical localization was quantified as to intensity staining distribution glandular epithelium, stroma, myometrium, results compared with those standard ligand binding assays. In all ER PgR localized within nuclei target cells. The maximal concentrations occurred mid- late...
Extranuclear estrogen receptor protein (estrophilin) of MCF-7 human breast cancer cells was purified by passage the cytosol fraction a cell homogenate through an affinity column estradiol linked to Sepharose substituted di-n-propyl sulfide bridge in 17 alpha position. Elution with 50 micro M [3H]estradiol 10% (vol/vol) dimethyl formamide/0.5 sodium thiocyanate gave 40% recovery [3H]estradiol-estrophilin showing 14% specific radioactivity expected for pure complex. Serum from Lewis rat...
To better understand direct and indirect androgen action on rat prostatic growth function, the various cell populations within intact adult ventral, dorsal, lateral prostate lobes were characterized for presence or absence of receptor (AR). Polyclonal rabbit antibodies raised against amino acids 1–21 AR (PG-21) used in combination with a library monoclonal directed cell-specific antigens positive cellular identification. Luminal epithelial cells strongly positive, an order ventral > ≥...
The second-generation antiandrogen enzalutamide was recently approved for patients with castration-resistant prostate cancer. Despite its success, the duration of response is often limited. For previous antiandrogens, one mechanism resistance mutation androgen receptor (AR). To prospectively identify AR mutations that might confer to enzalutamide, we performed a reporter-based mutagenesis screen and identified novel mutation, F876L, which converted into an agonist. Ectopic expression F876L...
Abstract Recent studies have identified somatic ESR1 mutations in patients with metastatic breast cancer and found some of them to promote estrogen-independent activation the receptor. The degree which all recurrent mutants can drive activities reduced sensitivity ER antagonists like fulvestrant is not established. In this report, we characterize spectrum from more than 900 patients. were detected 10%, D538G being most frequent (36%), followed by Y537S (14%). Several novel, activating also...
Somatic mutations in the estrogen receptor alpha (ERα) gene ( ESR1 ), especially Y537S and D538G, have been linked to acquired resistance endocrine therapies. Cell-based studies demonstrated that these mutants confer ERα constitutive activity antiestrogen suggest ligand-binding domain dysfunction leads therapy resistance. Here, we integrate biophysical structural biology data reveal how lead a constitutively active antiestrogen-resistant ERα. We show mutant ERs recruit coactivator absence of...
The most precise determination of the neutron lifetime using beam method was completed in 2005 and reported a result $\tau_n = (886.3 \pm 1.2 [\textrm{stat}] 3.2 [\textrm{syst}])$ s. dominant uncertainties were attributed to absolute fluence (2.7 s). measured with monitor that counted neutron-induced charged particles from absorption thin, well-characterized 6Li deposit. detection efficiency calculated areal density deposit, detector solid angle, evaluated nuclear data file, ENDF/B-VI...
Abstract A small pool of estrogen receptors (ERα and -β) localize at the plasma membrane rapidly signal to affect cellular physiology. Although nuclear ERs function mainly as homodimers, it is unknown whether membrane-localized ER exists or functions with similar requirements. We report that endogenous isoforms breast cancer endothelial cells exist predominantly homodimers in presence 17β-estradiol (E2). Interestingly, made from ERα /ERβ homozygous double-knockout mice, ERβ are absent,...