A5015112848- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Manufacturing Process and Optimization
- CRISPR and Genetic Engineering
- Protist diversity and phylogeny
- Augmented Reality Applications
- Plant biochemistry and biosynthesis
- Assembly Line Balancing Optimization
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Microbial Community Ecology and Physiology
- Plant Gene Expression Analysis
- Flexible and Reconfigurable Manufacturing Systems
- Human-Automation Interaction and Safety
- Biocrusts and Microbial Ecology
- Microbial Metabolic Engineering and Bioproduction
- Viral Infectious Diseases and Gene Expression in Insects
Heinrich Heine University Düsseldorf
2017-2023
Ernst Abbe University of Applied Sciences Jena
2022-2023
Carl Zeiss (United States)
2022
Cluster of Excellence on Plant Sciences
2017
Cyanobacteria are extremely adaptable, fast-growing, solar-powered cell factories that, like plants, able to convert carbon dioxide into sugar and oxygen thereby produce a large number of important compounds. Due their unique phototrophy-associated physiological properties, i.e. naturally occurring isoprenoid metabolic pathway, they represent highly promising platform for terpenoid biosynthesis. Here, we implemented carefully devised engineering strategy boost the biosynthesis commercially...
Cyclic triterpenes constitute one of the most diverse groups plant natural products. Besides intriguing biochemistry their biosynthetic pathways, exhibit versatile bioactivities, including antimicrobial effects against and human pathogens. While prokaryotes have been extensively used for heterologous production other classes terpenes, synthesis cyclic triterpenes, which inherently includes two-step catalytic formation universal linear precursor 2,3-oxidosqualene, is still a major challenge....
In cyanobacteria DNA supercoiling varies over the diurnal cycle and is integrated with temporal programs of transcription replication. We manipulated in Synechocystis sp. PCC 6803 by CRISPRi-based knockdown gyrase subunits overexpression topoisomerase I (TopoI). Cell division was blocked but cell growth continued all strains. The small endogenous plasmids were only transiently relaxed, then became strongly supercoiled TopoI strain. Transcript abundances showed a pronounced 5'/3' gradient...
Cyanobacteria are a promising platform for the production of triterpene squalene (C30), precursor all plant and animal sterols, highly attractive intermediate towards triterpenoids, large group secondary metabolites. Synechocystis sp. PCC 6803 natively produces from CO2 through MEP pathway. Based on predictions constraint-based metabolic model, we took systematic overexpression approach to quantify native gene's impact in squalene-hopene cyclase gene knock-out strain (Δshc). Our silico...
Die manuelle Montage ist zunehmend mit einer hohen Variantenvielfalt und kleinen Losgrößen konfrontiert. Sie muss eine ausgeprägte Flexibilität aufbringen, um den Qualitätsstandards individuellen Kundenanforderungen zu genügen. Um die Mitarbeiter Mitarbeiterinnen bei stetig komplexer werdenden Tätigkeiten unterstützen, kommen diverse Werkerassistenzsysteme zum Einsatz. Projektionsbasierte Assistenzsysteme wie Laser- oder Beamerprojektion erlauben kontextsensitive Informationsbereitstellung...
Kürzere Innovations- und Produktlebenszyklen bringen eine höhere Variantenvielfalt in die Produktion. Infolge dessen ändern sich Anforderungen an Gestaltung der manuellen Montage. Werker müssen kürzester Zeit befähigt werden, ein schnell wechselndes Produktportfolio fehlerfrei zu montieren. Zunehmend werden dafür diverse unterstützende Werkerassistenzsysteme eingesetzt. Dieser Beitrag veranschaulicht Potenziale informatorischer Werkerassistenzsysteme.   Shorter innovation and...
Abstract In cyanobacteria DNA supercoiling varies over the diurnal light/dark cycle and is integrated with temporal programs of transcription replication. We manipulated in Synechocystis sp. PCC 6803 by CRISPRi-based knock-down gyrase subunits overexpression topoisomerase I (TopoI), characterized phenotypes. Cell division was blocked, most likely due to inhibition genomic but not plasmid growth continued 4-5x wildtype cell volume, metabolic flux redirected towards glycogen TopoI strain....
The following protocol is optimized to generate first-strand cDNA for use in two step-PCR.
Removal of genomic DNA from RNA preparations
Removal of genomic DNA from RNA preparations
In this protocol, the extraction of naturally occurring as well heterologously synthesized triterpenes in Synechocystisand R. capsulatus are described
In this protocol, the extraction of template DNA out Synechocystis sp. PCC 6803 samples, PCR and application on a agarosegel are described. The protocol was handed over by Maximilian Dietsch MSc.
In this protocol, the extraction of naturally occurring as well heterologously synthesized triterpenes in Synechocystisand R. capsulatus are described
The following protocol is optimized to generate first-strand cDNA for use in two step-PCR.
Removal of genomic DNA from RNA preparations