A5037667234- Protist diversity and phylogeny
- HIV Research and Treatment
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- RNA Interference and Gene Delivery
Centre for Structural Systems Biology
2024
Heinrich Heine University Düsseldorf
2022
The tethering complexes HOPS/CORVET are central for vesicular fusion through the eukaryotic endolysosomal system, but functions of these in intracellular development malaria parasites still unknown. Here we show that core subunits critical proliferation parasite Plasmodium falciparum. We demonstrate required endocytosis and host cell cytosol uptake, as early functional depletion complex led to developmental arrest accumulation endosomes failed fuse digestive vacuole membrane. Late a severe...
In cyanobacteria DNA supercoiling varies over the diurnal cycle and is integrated with temporal programs of transcription replication. We manipulated in Synechocystis sp. PCC 6803 by CRISPRi-based knockdown gyrase subunits overexpression topoisomerase I (TopoI). Cell division was blocked but cell growth continued all strains. The small endogenous plasmids were only transiently relaxed, then became strongly supercoiled TopoI strain. Transcript abundances showed a pronounced 5'/3' gradient...
Post-synthetic modification of nucleic acid structures with clickable functionality is a versatile tool that facilitates many emerging applications, including immune evasion, enhancements in stability, fluorescent labelling, chemical 5'-RNA-capping and the development functional aptamers. While certain chemoenzymatic approaches for 3'-azido alkynyl labelling are known, equivalent 5'-strategies either inefficient, complex, or require harsh conditions. Here, we present modular facile...
The tethering complexes HOPS/CORVET are central for vesicular fusion through the eukaryotic endolysosomal system, but functions of these in intracellular development malaria parasites unknown. Here we show that early inactivation core complex subunits Plasmodium falciparum leads to developmental arrest and accumulation cytosolic vesicles, indicating a role parasite endocytosis endosomes digestive vacuole membrane. Late led mislocalization luminal rhoptry microneme proteins, severe defect...