A5034071792- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Evolution and Genetic Dynamics
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- Insect symbiosis and bacterial influences
- Gene Regulatory Network Analysis
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
Cancer Research UK
2023-2024
University of Cambridge
2023-2024
Delft University of Technology
2017-2022
Abstract The PIWI-interacting RNA (piRNA) pathway prevents endogenous genomic parasites, i.e. transposable elements, from damaging the genetic material of animal gonadal cells. Specific regions in genome, called piRNA clusters, are thought to define each species’ repertoire and therefore its capacity recognize silence specific transposon families. unistrand cluster flamenco ( flam ) is essential somatic compartment Drosophila ovary restrict Gypsy -family transposons infecting neighbouring...
ABSTRACT Animal germ cells deploy a specialized small RNA-based silencing system, called the PIWI-interacting RNA (piRNA) pathway, to prevent unwanted expression of transposable elements and maintain genome integrity. In Drosophila cells, majority piRNA populations originate from dual-strand clusters, genomic regions highly enriched in transposon fragments, via an elaborate protein machinery centred on heterochromatin 1 homolog, Rhino. Although Rhino binds peptides carrying trimethylated...
RNA interference (RNAi) is an indispensable mechanism for antiviral defense in insects, including mosquitoes that transmit human diseases. To escape this system, viruses encode suppressors of RNAi prevent elimination viral RNAs, and thus ensure efficient virus accumulation. Although the first animal Viral Suppressor (VSR) was identified more than a decade ago, molecular basis suppression by these proteins remains unclear. Here, we developed single-molecule fluorescence assay to investigate...
SUMMARY The PIWI-interacting RNA (piRNA) pathway prevents endogenous genomic parasites, transposable elements, from damaging the genetic material of animal gonadal cells. Specific regions in genome, called piRNA clusters, are thought to define each species’ repertoire and therefore its capacity recognize silence specific transposon families. unistrand cluster flamenco ( flam ) is essential somatic compartment Drosophila ovary restrict Gypsy -family transposons infecting neighbouring germ...
Abstract PIWI-interacting RNAs are a class of small non-coding that essential for transposon control in animal gonads. In Drosophila ovarian somatic cells, piRNAs transcribed from large genomic regions called piRNA clusters, which enriched fragments and acts as memory past invasions. Despite being widely present across species, clusters notoriously difficult to identify study due their lack sequence conservation limited synteny. Current identification methods rely either on extensive manual...
PIWI-interacting RNAs (piRNAs) are a class of small non-coding that essential for transposon control in animal gonads. In
Abstract CRISPR-Cas defence is a combination of adaptation to new invaders by spacer acquisition, and interference targeted nuclease activity. While these processes have been studied on population level, the individual cellular variability has remained unknown. Here, using microfluidic device combined with time-lapse microscopy, we monitor invader clearance in Escherichia coli across multiple generations. We observed that CRISPR fast narrow distribution times. In contrast, for escaping PAM...