A5087387338- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Insect Resistance and Genetics
- Genomics and Chromatin Dynamics
University of Cambridge
2018-2024
Cancer Research UK
2018-2024
The Francis Crick Institute
2024
Cancer Research UK Cambridge Center
2019-2021
The PIWI-interacting RNA (piRNA) pathway is a small RNA-based immune system that controls the expression of transposons and maintains genome integrity in animal gonads. In Drosophila, piRNA-guided silencing achieved, part, via co-transcriptional repression by Piwi. This depends on Panoramix (Panx); however, precisely how an binding event silences transcription remains to be determined. Here we show Nuclear Export Factor 2 (Nxf2) its co-factor, Nxt1, form complex with Panx are required for...
The PIWI-interacting RNA (piRNA) pathway is a conserved small RNA-based immune system that protects animal germ cell genomes from the harmful effects of transposon mobilization. In Drosophila ovaries, most piRNAs originate dual-strand clusters, which generate both genomic strands. Dual-strand clusters use noncanonical transcription mechanisms. Although transcribed by polymerase II, cluster transcripts lack splicing signatures and poly(A) tails. mRNA processing important for general export...
ABSTRACT Animal germ cells deploy a specialized small RNA-based silencing system, called the PIWI-interacting RNA (piRNA) pathway, to prevent unwanted expression of transposable elements and maintain genome integrity. In Drosophila cells, majority piRNA populations originate from dual-strand clusters, genomic regions highly enriched in transposon fragments, via an elaborate protein machinery centred on heterochromatin 1 homolog, Rhino. Although Rhino binds peptides carrying trimethylated...
The Piwi-interacting RNA (piRNA) pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires specialized machinery converts long single-stranded precursors into RNAs of ∼25-nucleotides length. This process involves factors operate two different subcellular compartments: the nuage/Yb body and mitochondria. How these sites communicate to achieve accurate substrate selection efficient processing remains unclear. Here, we...
In animal gonads, the PIWI-interacting RNA (piRNA) pathway guards genome integrity in part through co-transcriptional gene silencing of transposon insertions.
Abstract Eukaryotic DNA replication begins with the loading of MCM replicative helicase as a head-to-head double hexamer at origins 1–3 . Our current understanding how is assembled by origin recognition complex (ORC), CDC6 and CDT1 comes mostly from budding yeast. Here we characterize human (hDH) using biochemical reconstitution cryo-electron microscopy purified proteins. We show that engages differently yeast (yDH), generates approximately five base pairs underwound interface between...
Abstract The piRNA pathway is a conserved, small RNA-based immune system that protects animal germ cell genomes from the harmful effects of transposon mobilisation. In Drosophila ovaries, most piRNAs originate dual-strand clusters, which generate both genomic strands. Dual-strand clusters use non-canonical transcription mechanisms. Although transcribed by RNA polymerase II, cluster transcripts lack splicing signatures and poly(A) tails. mRNA processing important for general export mediated...
ABSTRACT The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. biogenesis requires specialised machinery converts long single-stranded precursors into RNAs of ~25-nucleotides length. This process involves factors operate two different subcellular compartments: the nuage/Yb-body and mitochondria. How these sites communicate to achieve accurate substrate selection efficient processing remains unclear. Here, we investigate previously...
Summary The PIWI-interacting RNA (piRNA) pathway is a small RNA-based immune system that controls the expression of transposons and maintains genome integrity in animal gonads. In Drosophila , piRNA-guided silencing achieved, part, via co-transcriptional repression by Piwi. This depends on Panoramix (Panx); however, precisely how an binding event silences transcription remains to be determined. Here we show Nuclear Export Factor 2 (Nxf2) its cofactor, Nxt1, form complex with Panx are...
Abstract In animal gonads, the PIWI-interacting RNA (piRNA) pathway guards genome integrity in part through co-transcriptional gene silencing of transposon insertions. Drosophila ovaries, piRNA-loaded Piwi detects nascent transcripts and instructs heterochromatin formation P anoramix- i nduced c o- t ranscriptional s ilencing (PICTS) complex, containing Panoramix, Nxf2 Nxt1. Here, we report that highly conserved dynein light chain LC8/Cut-up (Ctp) is an essential component PICTS complex....