A5061872645- Dermatology and Skin Diseases
- Plant Virus Research Studies
- Wound Healing and Treatments
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Nail Diseases and Treatments
- Plant and Fungal Interactions Research
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Diabetic Foot Ulcer Assessment and Management
- Psoriasis: Treatment and Pathogenesis
- Clostridium difficile and Clostridium perfringens research
- Acne and Rosacea Treatments and Effects
- Herpesvirus Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Disease Management and Epidemiology
- Allergic Rhinitis and Sensitization
- Paraoxonase enzyme and polymorphisms
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Antibiotic Resistance in Bacteria
- Bioinformatics and Genomic Networks
- Salmonella and Campylobacter epidemiology
- Antimicrobial Resistance in Staphylococcus
University of Maryland, College Park
2018-2021
Museum of Heilongjiang Province
2021
University of Pennsylvania
2015-2019
Jena University Hospital
2010
Viruses make up a major component of the human microbiota but are poorly understood in skin, our primary barrier to external environment. Viral communities have potential modulate states cutaneous health and disease. Bacteriophages known influence structure function microbial through predation genetic exchange. Human viruses associated with skin cancers multitude manifestations. Despite these important roles, little is regarding virome its interactions host microbiome. Here we evaluated...
The skin harbors complex communities of resident microorganisms, yet little is known their physiological roles and the molecular mechanisms that mediate cutaneous host-microbe interactions. Here, we profiled transcriptomes mice reared in presence absence microbiota to elucidate range pathways functions modulated by microbiota. A total 2820 genes were differentially regulated response microbial colonization enriched gene ontology (GO) terms related host-immune epidermal differentiation....
The skin microbiome is a complex ecosystem with important implications for cutaneous health and disease. Topical antibiotics antiseptics are often employed to preserve the balance of this population inhibit colonization by more pathogenic bacteria. However, despite their widespread use, impact these interventions on broader microbial communities remains poorly understood. Here, we report longitudinal effects topical bacterial role in Staphylococcus aureus resistance. In response antibiotics,...
<h3>Objectives</h3> Inducible costimulator (ICOS) and its ligand (ICOSL) regulate T B cell responses. Glucose-6-phosphate isomerase (G6PI)-induced arthritis requires lymphocytes. It was hypothesised that blocking ICOS/ICOSL interactions ameliorates G6PI-induced reduces G6PI-specific lymphocyte <h3>Methods</h3> DBA/1 mice were injected with a blocking, non-depleting anti-ICOSL monoclonal antibodies (mAbs) during the induction or effector phase of arthritis. antibody responses measured by...
Culture-independent analysis of microbial communities frequently relies on amplification and sequencing the prokaryotic 16S ribosomal RNA gene. Typical pipelines group sequences into operational taxonomic units (OTUs) to infer phylogenetic relationships. Here, we present HmmUFOtu, a novel tool for processing microbiome amplicon data, which performs rapid per-read placement, followed by phylogenetically informed clustering OTUs taxonomy assignment. Compared standard pipelines, HmmUFOtu more...
Localized genomic variability is crucial for the ongoing conflicts between infectious microbes and their hosts. An understanding of evolutionary adaptive patterns associated with will help guide development vaccines antimicrobial agents. While most analyses human microbiome have focused on taxonomic classification gene annotation, we investigated variation skin-associated viral communities. We evaluated across 16 healthy volunteers. Human papillomavirus (HPV) Staphylococcus phages contained...
Abstract Background Cholera has been present and recurring in Zambia since 1977. However, there is a paucity of data on genetic relatedness diversity the Vibrio cholerae isolates responsible for these outbreaks. Understanding whether outbreaks are seeded from existing local or if represent separate transmission events can inform public health decisions. Results Seventy-two V. 2009/2010, 2016, 2017/2018 were characterized using multilocus variable number tandem repeat analysis (MLVA) whole...
Abstract Diabetic foot ulcers (DFUs) are a major clinical problem exacerbated by prolonged bacterial infection. Macrophages, the primary innate immune cells, multifunctional cells that regulate diverse processes throughout multiple phases of wound healing. To better understand influence microbial species on macrophage behavior, we cultured human monocyte‐derived macrophages from four donors for 24 hours in media conditioned bacteria and fungi ( Pseudomonas aeruginosa , Corynebacterium...
Accurate predictions across multiple fields of microbiome research have far-reaching benefits to society, but there are few widely accepted quantitative tools make accurate about microbial communities and their functions. More discussion is needed the current state analysis required overcome hurdles preventing development implementation predictive analyses. We summarize ideas generated by participants Mid-Atlantic Microbiome Meet-up in January 2019. While it was clear from presentations that...
The advent of high throughput sequencing has enabled in-depth characterization human and environmental microbiomes. Determining the taxonomic origin microbial sequences is one first, frequently only, analysis performed on microbiome samples. Substantial research focused development methods for annotation, often making trade-offs in computational efficiency classification accuracy. A side-effect these efforts been a reexamination bacterial taxonomy itself. Taxonomies developed prior to...
The Mid-Atlantic Microbiome Meet-up (M3) organization brings together academic, government, and industry groups to share ideas develop best practices for microbiome research. In January of 2018, M3 held its fourth meeting, which focused on recent advances in biodefense, specifically those relating infectious disease, the use metagenomic methods pathogen detection. Presentations highlighted utility next-generation sequencing technologies identifying tracking microbial community members across...
High-throughput sequencing has revolutionized the field of microbiology, however, reconstructing complete genomes organisms from whole metagenomic shotgun data remains a challenge. Recovered are often highly fragmented, due to uneven abundances organisms, repeats within and across genomes, errors, strain-level variation. To address fragmented nature assemblies, scientists rely on process called binning, which clusters together contigs inferred originate same organism. Existing binning...
ABSTRACT Chronic, non-healing wounds are a major complication of diabetes associated with high morbidity and health care expenditures estimated at $9-13 billion annually in the US. Though microbial infection critical colonization is hypothesized to impair healing contribute severe outcomes such as amputation, antimicrobial therapy inefficacious role microbes tissue repair, regeneration, remains unclear. Here, longitudinal prospective cohort study 100 subjects non-infected neuropathic...
Abstract Objective: To test the feasibility of targeted gown and glove use by healthcare personnel caring for high-risk nursing-home residents to prevent Staphylococcus aureus acquisition in short-stay residents. Design: Uncontrolled clinical trial. Setting: This study was conducted 2 community-based nursing homes Maryland. Participants: The included 322 on mixed short- long-stay units. Methods: During a 2-month baseline period, all had nose inguinal fold swabs taken estimate S. acquisition....
Over the last decade, joint advances in next-generation sequencing technology and bioinformatics pipelines have dramatically improved our understanding of host-associated environmental microbiota. Standard microbiome community analysis typically involves amplicon prokaryotic 16S rRNA gene. These sequences are then clustered into operational taxonomic units (OTUs) for downstream diversity analyses, but also to reduce computational burden allow rapid datasets. Taxonomy is assigned all reads an...
ABSTRACT Localized genomic variability is crucial for the ongoing conflicts between infectious microbes and their hosts. An understanding of evolutionary adaptive patterns associated with will help guide development vaccines anti-microbial agents. While most analyses human microbiome have focused on taxonomic classification gene annotation, we investigated variation skin-associated viral communities. We evaluated across 16 healthy volunteers. HPV Staphylococcus phages contained 106 465...