A5082968382- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Nanopore and Nanochannel Transport Studies
- Air Quality Monitoring and Forecasting
- Gas Sensing Nanomaterials and Sensors
- Biosensors and Analytical Detection
- Advanced Chemical Sensor Technologies
- 3D Printing in Biomedical Research
- Electrical and Bioimpedance Tomography
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
- Electrowetting and Microfluidic Technologies
- Geophysical and Geoelectrical Methods
- Spectroscopy and Laser Applications
- Cellular Mechanics and Interactions
- Advanced Fiber Laser Technologies
- Advanced Machining and Optimization Techniques
- Solid State Laser Technologies
- Electrostatics and Colloid Interactions
- Advanced Fluorescence Microscopy Techniques
- Electrohydrodynamics and Fluid Dynamics
- Near-Field Optical Microscopy
- Meat and Animal Product Quality
- Electrokinetic Soil Remediation Techniques
- Plant Pathogens and Fungal Diseases
Nagoya University
2013-2020
Graduate School USA
2014
NTT (Japan)
1989
Chiba Prefectural Institute of Public Health
1982
Conventional concepts of resistive pulse analysis is to discriminate particles in liquid by the difference their size through comparing amount ionic current blockage. In sharp contrast, we herein report a proof-of-concept demonstration shape sensing capability solid-state pore sensors leveraging synergy between nanopore technology and machine learning. We found spikes similar patterns for two bacteria reflecting closely resembled morphology an ultra-low thickness-to-diameter aspect-ratio...
Measuring ionic currents passing through nano- or micropores has shown great promise for the electrical discrimination of various biomolecules, cells, bacteria, and viruses. However, conventional measurements have there is an inherent limitation to detectable particle volume (1% pore volume), which critically hinders applications real mixtures biomolecule samples with a wide size range suspended particles. Here we propose rational methodology that can detect 0.01% by measuring transient...
The detection of circulating tumor cells (CTCs) from liquid biopsies using microfluidic devices is attracting a considerable amount attention as new, less-invasive cancer diagnostic and prognostic method. One the drawbacks existing antibody-based systems false negatives for epithelial cell adhesion molecule CTCs. Here we report mechanical low-pass filtering technique based on constriction electrical current sensing system novel CTC in whole blood without any specific antigen-antibody...
Rapid detection of pathogenic bacteria is one the important social issues for preventing and identifying cause problems affecting human health. Ionic current sensing through pores has shown ability to electrically measure bacteria. However, there an inherent limitation discriminate chemical characteristics existing ionic methods Here we propose a real-time simultaneous measurement method, which combines fluorescence observation on microfluidic device. Our method can detect not only size...
The vibrational-rotational absorption of /sup 13/C/sub 2/H/sub 2/ molecules (VRAMs) in the 1.52-1.55 mu m region was investigated detail. On basis this investigation, frequency stabilization 1.55- distributed-feedback (DFB) laser diodes demonstrated. Frequency stability to within 2 MHz peak/peak fluctuation achieved at 1.54949- wavelength. In addition, stabilizations wavelength regions 1.53 and 1.54 were also carried out by using strong lines VRAM these regions. spectral width...
Ionic current sensing methods are useful tools for detecting sub- to several-micron scale particles such as bacteria. However, conventional commercially available ionic devices not suitable on-site measurement use because of inherent limitations on their robustness. Here, we proposed a portable robust sensor (Robust-ICS) using bridge circuit that offers high signal-to-noise (S/N) ratio by suppressing background current. Because the Robust-ICS can tolerate increased noise in sensing, simple,...
The molecular stretching of DNA is an indispensable tool for the optical exploration base sequences and epigenomic changes at a single molecule level. In terminal-unmodified molecules parallel to each other on solid substrate, receding meniscus assembly capillary force through dewetting process are quite useful. These can be achieved by pulling substrate out solution or sliding droplet between pair substrates. However, currently used methods do not allow control over liquid interface motion...
The mechanical properties of a cell, which include parameters such as elasticity, inner pressure, and tensile strength, are extremely important because changes in these indicative diseases ranging from diabetes to malignant transformation. Considering the heterogeneity within population cancer cells, robust measurement system at single cell level is required for research clinical purposes. In this study, potential microfluidic device high-throughput practical mechanotyping were developed...
We have demonstrated a PM2.5 analysis method that adds information on the number concentration and size by using microfluidic-based ionic current sensing with bridge circuit. The circuit allows for suppression of background detection small particles, even if relatively large micropore is used. This first demonstration particles via sensing; our enables analyses both size.
Bridge circuit based ionic current sensing in a microfluidic channel has attracted attention as highly sensitive analytical method for bio-related molecules and particles. However, geometry which greatly influences the detected not been investigated. Here, we investigate experimentally theoretically effect of differences on shapes amplitude signals sensing. Our results clarify geometrical bridge method.
A microfluidic device based on ionic current detection system for high-throughput and practical single bacteria mammalian cell sizing was developed, furthermore, discrimination of bacterial species deformability achieved. The highly precise blocking at narrow microchannel provided the information antibiotic resistant strains bacteria. Deformability changes associated with passage adipose tissue-derived stem cells (ASCs) were also successfully detected by without any chemical or biological...
Toward evaluating health risk of particulate matter with size less than or equal to 2.5 μm (PM2.5), we propose a methodology for realizing the PM2.5 analysis in liquid phase via water film-based collection and microfluidics-based electrical detection. We reported last year that hydrophilic nanowire surface was utilized formation film, which enabled continuous air-floating into followed-by As advanced results, collected successfully detected analyzed ion current sensing using micropore....