A5058762030- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Plant Pathogens and Resistance
- Environmental and Agricultural Sciences
- Forest, Soil, and Plant Ecology in China
- Advanced biosensing and bioanalysis techniques
- Genetic diversity and population structure
- Plant Ecology and Soil Science
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cytomegalovirus and herpesvirus research
- Plant Disease Resistance and Genetics
Max Planck Institute for Terrestrial Microbiology
2021-2024
Heidelberg University
2021
Nordwestdeutsche Forstliche Versuchsanstalt
1998
Abstract The mechanisms by which viruses hijack the genetic machinery of cells they infect are current interest. When bacteriophage T4 infects Escherichia coli , it uses three different adenosine diphosphate (ADP)-ribosyltransferases (ARTs) to reprogram transcriptional and translational apparatus host ADP-ribosylation using nicotinamide adenine dinucleotide (NAD) as a substrate 1,2 . NAD has previously been identified 5′ modification cellular RNAs 3–5 Here we report that ART ModB accepts not...
Bacteriophages are highly abundant viruses of bacteria. The major role phages in shaping bacterial communities and their emerging medical potential as antibacterial agents has triggered a rebirth phage research. To understand the molecular mechanisms by which hijack host, omics technologies can provide novel insights into organization transcriptional translational events occurring during infection process. In this study, we apply transcriptomics proteomics to characterize temporal patterns...
RNA modifications play essential roles in modulating function, stability, and fate across all kingdoms of life. The entirety the within a cell is defined as epitranscriptome. While eukaryotic are intensively studied, understanding bacterial remains limited, knowledge about bacteriophage almost nonexistent. In this review, we shed light on known mechanisms propose how might be extended to bacteriophages. We build hypotheses enzymes potentially responsible for regulating epitranscriptome...
Abstract Lytic bacteriophages hold substantial promise in medical and biotechnological applications. CRISPR-Cas systems offer a way to explore these mechanisms via site-specific phage mutagenesis. However, phages can resist Cas-mediated cleavage through extensive DNA modifications like cytosine glycosylation, hindering mutagenesis efficiency. Our study utilizes the eukaryotic enzyme NgTET temporarily reduce modifications, facilitating Cas nuclease enhancing This approach enables precise...
Lytic bacteriophages hold substantial promise in medical and biotechnological applications. Therefore a comprehensive understanding of phage infection mechanisms is crucial. CRISPR-Cas systems offer way to explore these via site-specific mutagenesis. However, phages can resist Cas-mediated cleavage through extensive DNA modifications like cytosine glycosylation, hindering mutagenesis efficiency. Our study utilizes the eukaryotic enzyme NgTET temporarily reduce modifications, facilitating Cas...
ABSTRACT Nicotinamide adenine dinucleotide (NAD) serves as a cap-like structure on cellular RNAs (NAD-RNAs) in all domains of life including the bacterium Escherichia coli . NAD also acts key molecule phage-host interactions, where bacterial immune systems deplete to abort phage infection. Nevertheless, NAD-RNAs have not yet been identified during infections bacteria and mechanisms their synthesis degradation are unknown this context. The T4 that specifically infects E. presents an important...
The mechanisms by which viruses hijack their host’s genetic machinery are of current interest. When bacteriophage T4 infects Escherichia coli , three different ARTs (ADP-ribosyltransferases) reprogram the transcriptional and translational apparatus through ADP-ribosylation using nicotinamide adenine dinucleotide (NAD) as substrate 1,2 . Recently, NAD was identified a 5’-modification cellular RNAs 3–5 Here, we report that ART ModB accepts not only but also NAD-capped RNA (NAD-RNA) attaches...
Abstract The mechanisms by which viruses hijack their host’s genetic machinery are of enormous current interest. One mechanism is adenosine diphosphate (ADP) ribosylation, where ADP-ribosyltransferases (ARTs) transfer an ADP-ribose fragment from the ubiquitous coenzyme nicotinamide adenine dinucleotide (NAD) to acceptor proteins. When bacteriophage T4 infects Escherichia coli, three different ARTs reprogram transcriptional and translational apparatus. Recently, NAD was identified as a...
Abstract Today, the clinical potential of antibiotics is almost exhausted, which has led to a renaissance phage research and therapy. To maximize therapy efficiency, we need an in-depth understanding molecular mechanisms by phages fight their hosts. Omics technologies can provide valuable insights in processes infection. We applied time-resolved proteomics transcriptomics characterize T4 infection Escherichia coli on level.