A5084249881- RNA Research and Splicing
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Fibroblast Growth Factor Research
- Neonatal Respiratory Health Research
- Genomics and Rare Diseases
- interferon and immune responses
- Immune cells in cancer
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
Center for Systems Biology
2022-2024
Harvard University
2021-2024
As tissues develop, cells divide and differentiate concurrently. Conflicting evidence shows that cell division is either dispensable or required for formation of types. To determine the role in differentiation, we arrested cycle zebrafish embryos using two independent approaches profiled them at single-cell resolution. We show differentiation all embryonic during initial type from early gastrulation to end segmentation. In absence division, slows down some types, exhibit global stress...
3’ untranslated region (3’UTR) variants are strongly associated with human traits and diseases, yet few have been causally identified. We developed the Massively Parallel Reporter Assay for 3’UTRs (MPRAu) to sensitively assay 12,173 3’UTR variants. applied MPRAu six cell lines, focusing on genetic genome-wide association studies (GWAS) evolutionary adaptation. expands our understanding of function, suggesting that low-complexity sequences predominately explain regulatory activity. adapt...
Abstract 3’ untranslated region (3’UTR) variants are strongly associated with human traits and diseases, yet few have been causally identified. We developed the Massively Parallel Reporter Assay for 3’UTRs (MPRAu) to sensitively assay 12,173 3’UTR variants. applied MPRAu six cell lines, focusing on genetic genome-wide association studies (GWAS) evolutionary adaptation. expands our understanding of function, suggesting that low-complexity sequences predominately explain regulatory activity....
Receptor-mediated signaling plays a central role in tissue regeneration, and it is dysregulated disease. Here, we build signaling-response map for model regenerative human tissue: the airway epithelium. We analyzed effect of 17 receptor-mediated pathways on organotypic cultures to determine changes abundance phenotype all epithelial cell types. This recapitulates gamut known responses these pathways. It defines convergent states induced by multiple ligands diverse, ligand-specific basal-cell...