The growing world population demands an increase in animal protein production. Seaweed may be a valuable source of for feed. However, biorefinery approach aimed at cascading valorisation both and non-protein seaweed constituents is required to realise economically feasible value chain. In this study, such presented the green Ulva lactuca containing 225 g (N × 4.6) kg−1 dry matter (DM). sugars biomass were solubilised by hot water treatment followed enzymatic hydrolysis centrifugation...

CRISPR/Cas-based genetic engineering has revolutionised molecular biology in both eukaryotes and prokaryotes. Several tools dedicated to the genomic transformation of Clostridium genus Gram-positive bacteria have been described literature; however, integration large DNA fragments still remains relatively limited. In this study, a CRISPR/Cas9 genome editing tool using two-plasmid strategy was developed for solventogenic strain acetobutylicum ATCC 824. Codon-optimised cas9 from Streptococcus...

Abstract Isopropanol represents a widely-used commercial alcohol which is currently produced from petroleum. In nature, isopropanol excreted by some strains of Clostridium beijerinckii , simultaneously with butanol and ethanol during the (IBE) fermentation. order to increase production, gene encoding secondary-alcohol dehydrogenase enzyme C. NRRL B593 ( adh ) catalyzes reduction acetone isopropanol, was cloned into acetone, (ABE)-producing strain acetobutylicum ATCC 824. The transformants...

Artificial designer minicellulosomes comprise a chimeric scaffoldin that displays an optional cellulose-binding module (CBM) and bacterial cohesins from divergent species which bind strongly to enzymes engineered bear complementary dockerins. Incorporation of cellulosomal cellulases Clostridium cellulolyticum into leads artificial complexes with enhanced activity on crystalline cellulose, due enzyme proximity substrate targeting induced by the scaffoldin-borne CBM. In present study, dockerin...

In microorganisms, the enzyme acetate kinase (AK) catalyses formation of ATP from ADP by de-phosphorylation acetyl phosphate into acetic acid. A mutant strain Clostridium acetobutylicum lacking activity is expected to have reduced and acetone production compared wild type. this work, a C. with selectively disrupted ack gene, encoding AK, was constructed genetically physiologically characterized. The (-) showed reduction in more than 97% fermentation profiles wild-type were using two...

Acetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions, Clostridium acetobutylicum ATCC 824 (and strains derived from it) generates both d- and l-stereoisomers acetoin, but because absence an ACR enzyme, it does not produce A gene encoding beijerinckii NCIMB 8052 was functionally expressed in C. under control two strong promoters, constitutive thl promoter late exponential adc promoter. Both ACR-overproducing were grown batch cultures, during...

An exploratory Life Cycle Assessment (LCA) was carried out to provide insight into the environmental impacts of using green seaweed Ulva spp. as a feedstock, for production bioplastic. The study focused on lactic acid precursor polylactic acid. process: (1) cultivation spp., in an Integrated Multitrophic Aquaculture system; (2) processing biomass solubilization sugars; (3) fermentation sugars acid; (4) isolation from broth. identified hotspots and compared experimental chain with...

Random mutagenesis and genome shuffling was applied to improve solvent tolerance isopropanol/butanol/ethanol (IBE) production in the strictly anaerobic bacteria Clostridium beijerinckii DSM 6423. Following chemical with N-methyl-N-nitro-N-nitrosoguanidine (NTG), screening of putatively improved strains done by submitting mutants toxic levels inhibitory chemicals or for their isopropanol (>35 g/L). Suicide substrates, such as ethyl methyl bromobutyrate alcohol dehydrogenase inhibitors like...

This research assesses the well-to-tank (WTT) greenhouse gas (GHG) emissions and economic performance of an innovative bio-jet fuel via acetone-butanol-ethanol (ABE) fermentation. Dutch potato by-products from food processing industry sugar beets are explored as potential feedstocks. Four product systems differentiated by feedstocks, logistics centralized/decentralized fermenters investigated. For both it is found that a centralized large-scale fermentation preferable to decentralized...

To mitigate the climate change impact of aviation, jet fuels from bio-based by-products are considered a promising alternative to conventional fuels. Life cycle assessment (LCA) is commonly applied tool determine environmental impacts bio-jet This article presents both attributional and consequential LCA models assess an innovative fuel produced potato in Netherlands. The two led opposite conclusions regarding overall performance this fuel. showed that could offer about 60% GHG emissions...

Seaweed (or macroalgae) produced sustainably at large scale opens opportunities as source of fuels, chemicals and food. The production does not directly compete with terrestrial food may make use anthropogenic sources carbon dioxide nitrogen. biomass can be transformed into a suitable substrate for fermentation using biorefinery approach. In this study the entire process biofuel from seaweed is described: starting cultivation harvest, dried cut, enzymatically hydrolysed, demineralized,...

Seaweeds (macroalgae) are gaining attention as potential sustainable feedstock for the production of fuels and chemicals. This comparative study focuses on characterization microbial alcohols from fermentable carbohydrates in hydrolysate macroalgae Laminaria digitata raw material. The a carbon source selected was tested, using three physiologically different fermentative microbes, two main types processes. For ethanol, Saccharomyces cerevisiae used benchmark microorganism compared with...

ABSTRACT Clostridium acetobutylicum ATCC 824 is a solventogenic bacterium that grows heterotrophically on variety of carbohydrates, including glucose, cellobiose, xylose, and lichenan, linear polymer β-1,3- β-1,4-linked β- d -glucose units. C. does not degrade cellulose, although its genome sequence contains several cellulase-encoding genes complete cellulosome cluster genes. In the present study, we demonstrate low but significant level induction cellulase activity occurs during growth...

There is a worldwide interest for sustainable and environmentally-friendly ways to produce fuels chemicals from renewable resources. Among them, the production of acetone, butanol ethanol (ABE) or Isopropanol, Butanol Ethanol (IBE) by anaerobic fermentation has already long industrial history. Isopropanol recently received specific best studied natural isopropanol producer C. beijerinckii DSM 6423 (NRRL B-593). This strain metabolizes sugars into mix IBE with only low concentrations produced...

Second generation bioethanol represents an important biofuel, but innovations on biomass pre-treatment and fermentation are needed to improve efficiency cost-effectiveness in its production. In this work, beech wood chips were treated using acetone-based organosolv fractionation process at pilot scale, resulting the isolation of lignin, a high-purity cellulosic pulp liquid stream containing hemicellulose sugars. The (C5 stream) contained xylose as major sugar also inhibitors such furanics,...