A5019907039- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Magnesium in Health and Disease
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Cell Adhesion Molecules Research
- Ion Channels and Receptors
- Cell Image Analysis Techniques
- Phosphodiesterase function and regulation
- Ion channel regulation and function
- Cellular Mechanics and Interactions
- Erythrocyte Function and Pathophysiology
- Signaling Pathways in Disease
- Advanced biosensing and bioanalysis techniques
- Microtubule and mitosis dynamics
- Neuroscience and Neuropharmacology Research
- Advanced Biosensing Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Mechanisms of cancer metastasis
- Protease and Inhibitor Mechanisms
- Photoreceptor and optogenetics research
- Peptidase Inhibition and Analysis
- bioluminescence and chemiluminescence research
The Netherlands Cancer Institute
2016-2025
Oncode Institute
1992-2025
University of Amsterdam
2013-2024
Van Leeuwenhoek Centre for Advanced Microscopy
2011-2024
Institute of Life Sciences
2023
Amsterdam University Medical Centers
2023
Netherlands Institute for Neuroscience
2016
Dutch Cancer Society
2016
Cancer Genomics Centre
2001-2016
Institute of Cell Biology
2014
Lysophosphatidic acid (LPA) is a water-soluble phospholipid with hormone-like and growth-factor-like activities. LPA activates putative G-protein-coupled receptor in responsive cells, but the natural source of exogenous unknown. Here we show that present mammalian serum an active form (bound to albumin) at concentrations 1-5 microM, not detectable platelet-poor plasma, suggesting produced during blood clotting. We find thrombin activation platelets prelabelled [32P]Pi results rapid release...
Addition of the bioactive phospholipid lysophosphatidic acid (LPA) or a thrombin receptor-activating peptide (TRP) to serum-starved N1E-115 NG108-15 neuronal cells causes rapid growth cone collapse, neurite retraction, and transient rounding cell body. These shape changes appear be driven by receptor-mediated contraction cortical actomyosin system independent classic second messengers. Treatment with Clostridium botulinum C3 exoenzyme, which ADP-ribosylates thereby inactivates Rho small...
Epac-based FRET sensors have been widely used for the detection of cAMP concentrations in living cells. Originally developed by us as well others, we since then reported several important optimizations that make these favourite among many cell biologists. We here report cloning and characterization our fourth generation sensors, which feature outstanding photostability, dynamic range signal-to-noise ratio. The design is based on mTurquoise2, currently brightest most bleaching-resistant...
Abstract Autophagy is the main homeostatic pathway guiding cytosolic materials for degradation by lysosome. Maturation of autophagosomes requires their transport towards perinuclear region cell, with key factors underlying both processes still poorly understood. Here we show that and positioning late depends on cholesterol way cholesterol-sensing Rab7 effector ORP1L. ORP1L localizes to and—under low-cholesterol conditions—contacts ER protein VAP-A, forming ER-autophagosome contact sites,...
Single Molecule Localization super-resolution Microscopy (SMLM) has become a powerful tool to study cellular architecture at the nanometer scale. In SMLM, single fluorophore labels are made repeatedly switch on and off ("blink"), their exact locations determined by mathematically finding centers of individual blinks. The image quality obtainable SMLM critically depends efficacy blinking (brightness, fraction molecules in on-state) preparation longevity labeling density. Recent work...
Surface metallization by plasma coating enhances desorption/ionization of membrane components such as lipids and sterols in imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) tissues cells. High-resolution images cholesterol other were obtained for neuroblastoma cells revealed subcellular details (resolving power 1.5 μm). Alternatively, matrix-enhanced SIMS, 2,5-dihydroxybenzoic acid electrosprayed on allowed intact molecular phosphatidylcholine sphingomyelin at the cellular...
Lysophosphatidic acid (LPA) is a naturally occurring phospholipid with growth-factor-like activities [van Corven, Groenink, Jalink, Eichholtz & Moolenaar (1989) Cell 45, 45-54]. We have examined various structural analogues of LPA for their ability to stimulate DNA synthesis in quiescent fibroblasts. When the acyl-chain length varied, rank order mitogenic potency is: 1-oleoyl congruent 1-palmitoyl greater than 1-myristoyl 1-lauroyl 1-decanoyl LPA; last compound shows almost no activity over...
Lysophosphatidic acid (LPA) is a potent mitogen for quiescent fibroblasts. Among the earliest detectable responses to LPA GTP-dependent phosphoinositide hydrolysis (van Corven, E. J., Groenink, A., Jalink, K., Eichholtz, T., and Moolenaar, W. H. (1989) Cell 59, 45-54). Here we describe Ca2(+)-mobilizing properties of in human fibroblasts present evidence suggesting that previously reported effects phosphatidic are attributable contamination with LPA. Addition (1-oleoyl or 1-palmitoyl) evokes...
Agonist-induced intracellular Ca2+ signals following phospholipase C (PLC) activation display a variety of patterns, including transient, sustained, and oscillatory behavior. These changes have been well characterized, but detailed kinetic analyses PLC in single living cells is lacking, due to the absence suitable indicators for use vivo. Recently, green fluorescent protein-tagged pleckstrin homology domains employed monitor cells, based on (confocal) imaging their fluorescence translocation...