A5057609112- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Ion-surface interactions and analysis
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Analytical chemistry methods development
- Nuclear Physics and Applications
- Osteoarthritis Treatment and Mechanisms
- Cultural Heritage Materials Analysis
- Forensic Fingerprint Detection Methods
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Chemical Sensor Technologies
- Particle accelerators and beam dynamics
- Molecular Biology Techniques and Applications
- Forensic Toxicology and Drug Analysis
- Medical Imaging Techniques and Applications
- Isotope Analysis in Ecology
- Conservation Techniques and Studies
- Atomic and Molecular Physics
- Cancer, Lipids, and Metabolism
- Microfluidic and Capillary Electrophoresis Applications
- Building materials and conservation
- Forensic and Genetic Research
- Spectroscopy and Chemometric Analyses
- Integrated Circuits and Semiconductor Failure Analysis
Maastricht University
2016-2025
Imaging Center
2017-2024
Technical University of Munich
2024
Institute for Atomic and Molecular Physics
2009-2018
Hertie Institute for Clinical Brain Research
2013
University of Tübingen
2013
University Hospital of Zurich
2013
Utrecht University
2002-2012
University of Lucknow
2012
Medical University of Warsaw
2012
Imaging mass spectrometry combines the chemical specificity and parallel detection of with microscopic imaging capabilities. The ability to simultaneously obtain images from all analytes detected, atomic macromolecular ions, allows analyst probe organization a sample correlate this physical features. sensitivity ionization step, preparation, spatial resolution, speed technique are important parameters that affect type information obtained. Recently, significant progress has been made in each...
Mass spectrometry imaging (MSI) enables the spatial distributions of molecules possessing different mass-to-charge ratios to be mapped within complex environments revealing regional changes at molecular level. Even high mass resolving power, however, these images often reflect summed distribution multiple isomeric molecules, each potentially a unique coinciding with distinct biological function(s) and metabolic origin. Herein, this chemical ambiguity is addressed through an innovative...
Abstract Grain boundaries play a key role in the performance of thin‐film optoelectronic devices and yet their effect halide perovskite materials is still not understood. The biggest factor limiting progress inability to identify grain boundaries. Noncrystallographic techniques can misidentify boundaries, leading conflicting literature reports about influence; however, gold standard – electron backscatter diffraction (EBSD) destroys thin films. Here, this problem solved by using solid‐state...
Human cells produce thousands of lipids that change during cell differentiation and can vary across individual the same type. However, we are only starting to characterize function these cell-to-cell differences in lipid composition. Here, measured lipidomes transcriptomes human dermal fibroblasts by coupling high-resolution mass spectrometry imaging with single-cell transcriptomics. We found variations specific metabolic pathways contribute establishment states involved organization skin...
Recently, a novel technology was published, utilizing the strengths of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) and immunohistochemistry (IHC), achieving highly multiplexed, targeted biomolecules in tissue. This new technique, called MALDI-IHC, opened up workflows to target molecules interest using MALDI-MSI that are usually by standard IHC. In this paper, utility MALDI-IHC its complementarity with untargeted on-tissue bottom-up spatial proteomics...
Surface metallization by plasma coating enhances desorption/ionization of membrane components such as lipids and sterols in imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) tissues cells. High-resolution images cholesterol other were obtained for neuroblastoma cells revealed subcellular details (resolving power 1.5 μm). Alternatively, matrix-enhanced SIMS, 2,5-dihydroxybenzoic acid electrosprayed on allowed intact molecular phosphatidylcholine sphingomyelin at the cellular...
For the first time macromolecular ion microscope images have been recorded using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Single-shot, mass-resolved of spatial distributions intact peptide and protein ions over an area 200 μm in diameter were obtained less than 1 ms at a repetition rate 12 Hz. The magnifying optics allowed to be with lateral resolution 4 μm. These results prove concept high-resolution MALDI-MS imaging mode without need for tight focus...
MALDI imaging mass spectrometry (MALDI-IMS) has become a powerful tool for the detection and localization of drugs, proteins, lipids on-tissue. Nevertheless, this approach can only perform identification low molecules as lipids, pharmaceuticals, peptides. In article, combination approaches proteins their directly on-tissue is described after tryptic digestion. Enzymatic digestion protocols different kinds tissues—formalin fixed paraffin embedded (FFPE) frozen tissues—are combined with...
The imaging capabilities of time-of-flight secondary ion mass spectrometry (ToF−SIMS) and MALDI-MS sample preparation methods were combined. We used this method, named matrix-enhanced (ME) SIMS, for direct molecular nervous tissue at micrometer spatial resolution. Cryosections the cerebral ganglia freshwater snail Lymnaea stagnalis placed on indium−tin−oxide (ITO)-coated conductive glass slides covered with a thin layer 2,5-dihydroxybenzoic acid by electrospray deposition. High-resolution...
Background Hypertension is, amongst others, characterized by endothelial dysfunction and vascular remodeling. As sphingolipids have been implicated in both the regulation of contractility growth, we investigated whether sphingolipid biology is altered hypertension this reflected function. Methods Findings In isolated carotid arteries from spontaneously hypertensive rats (SHR) normotensive Wistar-Kyoto (WKY) rats, shifting ceramide/S1P ratio towards ceramide dominance administration a...
Imaging mass spectrometry provides both chemical information and the spatial distribution of each analyte detected. Here it is demonstrated how imaging tissue at subcellular resolution can be achieved by combining high secondary ion (SIMS) with sample preparation protocols matrix-assisted laser desorption/ionization (MALDI). Despite mechanistic differences sampling 10(5) times less material, matrix-enhanced (ME)-SIMS samples yields similar results to MALDI (up m/z 2500), in agreement...
Mass spectrometric imaging (MSI) is an analytical technique used to determine the distribution of individual analytes within a given sample. A wide array and samples can be investigated by MSI, including drug in rats, lipid analysis from brain tissue, protein differentiation tumors, plant metabolite distributions. Matrix-assisted laser desorption/ionization (MALDI) soft ionization capable desorbing ionizing large range compounds, it most common source MSI. MALDI mass spectrometry (MS)...
Analytical methods are pursued to measure the identity and location of biomolecules down subcellular (microm) level. Available mass spectrometric imaging either compromise localization accuracy or identification in their analysis surface biomolecules. In this study, FTICR-MS is applied for spatially resolved rat brain tissue with aim optimize protein by high online MS/MS capabilities technique. Mass accuracies up 6 ppm were obtained direct MALDI-analysis together a spatial resolution 200...
The lipid compositions of different breast tumor microenvironments are largely unknown due to limitations in imaging techniques. Imaging distributions would enhance our understanding processes occurring inside growing tumors, such as cancer cell proliferation, invasion, and metastasis. Recent developments MALDI mass spectrometry (MSI) enable rapid specific detection lipids directly from thin tissue sections. In this study, we performed multimodal acylcarnitines, phosphatidylcholines (PC), a...
In mass spectrometry imaging (MSI) it is often desirable to analyse the same sample in both polarities extract most information. However, many matrices that produce high-quality spectra matrix-assisted laser desorption/ionization (MALDI) are volatile, greatly limiting their use long experiments. We demonstrate using a new high speed MALDI-MSI instrument, volatile matrices, including those intense lipid signals positive and negative ion mode, can now be effectively used MSI.A prototype Bruker...
Single-cell analysis provides insights into cellular heterogeneity and dynamics of individual cells. This Feature highlights recent developments in key analytical techniques suited for single-cell metabolic with a special focus on mass spectrometry-based platforms RNA-seq as well imaging that reveal stochasticity metabolism.