A5005018253- Mass Spectrometry Techniques and Applications
- Ion-surface interactions and analysis
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- X-ray Spectroscopy and Fluorescence Analysis
- Forensic Fingerprint Detection Methods
- Molecular Biology Techniques and Applications
- Nuclear Physics and Applications
- Analytical chemistry methods development
- Ferroptosis and cancer prognosis
- Forensic and Genetic Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Electron and X-Ray Spectroscopy Techniques
- Diversity and Impact of Dance
- Glioma Diagnosis and Treatment
- GABA and Rice Research
- Isotope Analysis in Ecology
- Nanoparticles: synthesis and applications
- Integrated Circuits and Semiconductor Failure Analysis
- Analytical Chemistry and Chromatography
- Musicians’ Health and Performance
- Plant-Microbe Interactions and Immunity
- Cannabis and Cannabinoid Research
- Vitamin D Research Studies
- Winter Sports Injuries and Performance
Université de Lille
2019-2024
Centre Hospitalier Universitaire de Lille
2020-2024
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2019-2024
Inserm
2019-2024
Maastricht University
2015-2022
Jožef Stefan Institute
2012-2017
Institute for Atomic and Molecular Physics
2015
Gold nanoparticles (GNPs) are claimed as outstanding biomedical tools for cancer diagnostics and photo-thermal therapy, but without enough evidence on their potentially adverse immunological effects. Using a model of human dendritic cells (DCs), we showed that 10 nm- 50 nm-sized GNPs (GNP10 GNP50, respectively) were internalized predominantly via dynamin-dependent mechanisms, they both impaired LPS-induced maturation allostimulatory capacity DCs, although the effect GNP10 was more prominent....
In mass spectrometry imaging (MSI) it is often desirable to analyse the same sample in both polarities extract most information. However, many matrices that produce high-quality spectra matrix-assisted laser desorption/ionization (MALDI) are volatile, greatly limiting their use long experiments. We demonstrate using a new high speed MALDI-MSI instrument, volatile matrices, including those intense lipid signals positive and negative ion mode, can now be effectively used MSI.A prototype Bruker...
We report a method for the unambiguous identification of molecules in biological and materials specimens at high practical lateral resolution using new TOF-SIMS parallel imaging MS/MS spectrometer. The tandem mass spectrometry reported here is based on precise monoisotopic selection precursor ions from secondary ion stream followed by synchronous collection product data. Thus, our enables simultaneous surface screening complex matrix chemistry with (MS(1)) targeted components (MS(2))...
Abstract Rapid and accurate clinical diagnosis remains challenging. A component of tool development is the design effective classification models with Mass spectrometry (MS) data. Some Machine Learning approaches have been investigated but these require time-consuming preprocessing steps to remove artifacts, making them unsuitable for rapid analysis. Convolutional Neural Networks (CNNs) found perform well under such circumstances since they can learn representations from raw However, their...
Abstract Molecular heterogeneity is a key feature of glioblastoma that impedes patient stratification and leads to large discrepancies in mean survival. Here, we analyze cohort 96 patients with survival ranging from few months over 4 years. 46 tumors are analyzed by mass spectrometry-based spatially-resolved proteomics guided spectrometry imaging. Integration protein expression clinical information highlights three molecular groups associated immune, neurogenesis, tumorigenesis signatures...
With a model invertebrate animal, we have assessed the fate of magnetic nanoparticles in biologically relevant media, i.e., digestive juices. The toxic potential and internalization such by nontarget cells were also examined. aim this study was to provide experimental evidence on formation Co(2+), Fe(2+), Fe(3+) ions from CoFe₂O₄ juices organism. Standard toxicological parameters assessed. Cell membrane stability tested with modified method for measurement its quality. Proton-induced X-ray...
The high ion signals produced by many lipids in mass spectrometry imaging (MSI) make them an ideal molecular class to study compositional changes throughout tissue sections and their relationship with disease. However, the large extent of structural diversity observed lipidome means optimal signal for different lipid classes is often obtained opposite polarities. In this work we demonstrate how new speed MALDI-MSI technologies combined precise laser position control enables acquisition...
Matrix-enhanced secondary ion mass spectrometry (ME-SIMS) has overcome one of the biggest disadvantages SIMS analysis by providing ability to detect intact biomolecules at high spatial resolution. By increasing ionization efficiency and minimizing primary beam-induced fragmentation analytes, ME-SIMS proven useful for detection numerous biorelevant species, now including peptides. We report here first demonstration tandem de novo sequencing endogenous neuropeptides from tissue in situ (i.e.,...
Mass spectrometry imaging (MSI) has shown to bring invaluable information for biological and clinical applications. However, conventional MSI is generally performed ex vivo from tissue sections. Here, we developed a novel MS-based method in mass imaging. By coupling the SpiderMass technology, that provides minimally invasive analysis–to robotic arm of high accuracy, demonstrate images can be acquired any surface by moving laser probe above surface. equipping with sensor, are also able both...
Oral cavity cancers are the 15th most common cancer with more than 350,000 new cases and ~178,000 deaths each year. Among them, squamous cell carcinoma (SCC) accounts for 90% of tumors located in oral on oropharynx. For SCC, surgical resection remains primary course treatment. Generally, margins defined intraoperatively using visual tactile elements. However, 15-30% cases, positive found after histopathological examination several days postsurgery. Technologies based mass spectrometry (MS)...
Direct analysis by mass spectrometry (imaging) has become increasingly deployed in preclinical and clinical research due to its rapid accurate readouts. However, when it comes biomarker discovery or histopathological diagnostics, more sensitive in-depth profiling from localized areas is required. We developed a comprehensive, fully automated online platform for high-resolution liquid extraction surface (HR-LESA) followed micro-liquid chromatography (LC) separation data-independent...
Mass spectrometry imaging (MSI) is a powerful molecular technique. In microprobe MSI, images are created through grid-wise interrogation of individual spots by mass across surface. Classical statistical tests for within-sample comparisons fail as close-by measurement violate the assumption independence these tests, which can lead to an increased false-discovery rate. For spatial data, this effect referred autocorrelation. study, we investigated autocorrelation in three different...
Detecting different lipid profiles in early infarct development may give an insight on the fate of compromised tissue. Here we used Mass Spectrometry Imaging to identify lipids at 4, 8 and 24 hours after ischemic stroke mice, induced by transient middle cerebral artery occlusion (tMCAO). Combining linear transparency overlay, a clustering pipeline spatial segmentation, identified three regions: core, penumbra (i.e. comprised tissue that is not yet converted core), surrounding healthy...
The first calculations of the similarity and correlation lipid images between MALDI WALDI for evaluating a common ground truth in MS imaging.
Ballet dancers are expected to use their bodies symmetrically during training, because dance movements performed on both sides. However, there is a general belief that ballet training encourages the of one side body more than other. Frequent repetition particular exercise can lead asymmetries and musculoskeletal injuries. The aim this cross-sectional study was investigate presence lower limbs trunk muscle strength in secondly assess whether difference between professional students.
There is growing interest in limiting the use of fungicides and implementing innovative, environmentally friendly strategies, such as beneficial bacteria-triggered immunity, to protect grapevines from natural pathogens. Therefore, we need rapid innovative ways translate knowledge molecular mechanisms underlying activation grapevine defenses against pathogens induced resistance. Here, have implemented an
Mass spectrometry imaging (MSI) is a label free technique capable of providing simultaneous identification and localization biomolecules. A multimodal approach required that allows for the study complexity biological tissue samples to overcome limitations single MSI technique. Secondary ion mass (SIMS) high spatial resolution while matrix-assisted laser desorption (MALDI) offers significantly wider range. The combination coregistered SIMS MALDI images results in detailed unique biomolecular...