A5075855632- Immunotherapy and Immune Responses
- Nanoparticles: synthesis and applications
- Immune cells in cancer
- Immune Response and Inflammation
- Mesenchymal stem cell research
- Immune Cell Function and Interaction
- Advanced Cellulose Research Studies
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Macrophage Migration Inhibitory Factor
- T-cell and B-cell Immunology
- Graphene and Nanomaterials Applications
- Polysaccharides and Plant Cell Walls
- Endodontics and Root Canal Treatments
- Pomegranate: compositions and health benefits
- Extracellular vesicles in disease
- COVID-19 Clinical Research Studies
- Gut microbiota and health
- Wound Healing and Treatments
- Oral and Maxillofacial Pathology
- Advanced Glycation End Products research
- Shape Memory Alloy Transformations
- Parasitic Diseases Research and Treatment
- Anesthesia and Neurotoxicity Research
University of Belgrade
2017-2025
Institute for the Application of Nuclear Energy
2020-2023
Military Medical Academy
2010-2019
University of Defence
2012-2015
ACTA (United States)
2015
Adult mesenchymal stem cells (MSCs) have recently become a potent tool in regenerative medicine. Due to certain shortcomings of obtaining bone marrow MSCs, alternate sources MSCs been sought. In this work, we studied from dental pulp (DP-MSCs) and follicle (DF-MSCs), isolated the same tooth/donor, define differences their phenotypic properties, differentiation potential, immunomodulatory activities. Both cell types showed colony-forming ability expressed typical markers, but differed levels...
Gold nanoparticles (GNPs) are claimed as outstanding biomedical tools for cancer diagnostics and photo-thermal therapy, but without enough evidence on their potentially adverse immunological effects. Using a model of human dendritic cells (DCs), we showed that 10 nm- 50 nm-sized GNPs (GNP10 GNP50, respectively) were internalized predominantly via dynamin-dependent mechanisms, they both impaired LPS-induced maturation allostimulatory capacity DCs, although the effect GNP10 was more prominent....
Myeloid-derived suppressor cells (MDSC) emerged as major factors driving the tumor progression due to numerous immunosuppressive mechanisms they possess. Prostaglandin (PG)E2 is shown critical for induction of MDSC and their suppressive functions in vivo, but it poorly understood how affects capacity induce different subsets regulatory T (Treg). By using a novel protocol generation mononuclear (M)-MDSC, we showed that PGE2 potentiates GM-CSF/IL-6-dependent CD33+CD11b+HLA-DR-CD14+ M-MDSC...
Widespread coronavirus disease (COVID)-19 is causing pneumonia, respiratory and multiorgan failure in susceptible individuals. Dysregulated immune response marks severe COVID-19, but the immunological mechanisms driving COVID-19 pathogenesis are still largely unknown, which hampering development of efficient treatments. Here we analyzed ~140 parameters cellular humoral peripheral blood 41 patients 16 age/gender-matched healthy donors by flow-cytometry, quantitative PCR, western blot ELISA,...
Abstract Cellulose nanofibrills (CNFs) are attractive biocompatible, natural nanomaterials for wide biomedical applications. However, the immunological mechanisms of CNFs have been poorly investigated. Considering that dendritic cells (DCs) key immune regulatory in response to nanomaterials, our aim was investigate a model DC-mediated response. We found non-toxic concentrations impaired differentiation and subsequent maturation human monocyte-derived (mo)-DCs. In co-culture with CD4 + T...
Trichinella spiralis, as well its muscle larvae excretory-secretory products (ES L1), given either alone or via dendritic cells (DCs), induce a tolerogenic immune microenvironment in inbred rodents and successfully ameliorate experimental autoimmune encephalomyelitis. ES L1 directs the immunological balance away from T helper (Th)1, toward Th2 regulatory responses by modulating DCs phenotype. The ultimate goal of our work is to find out if it possible translate knowledge obtained animal...
Abstract The characterization of mechanisms involved in the positive effects probiotic bacteria various pathophysiological conditions is a prerogative for their safe and efficient application biomedicine. We have investigated immunological live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an vitro model activated immune cells. shown that GABA containing GABA-free strong...
Autologous dendritic cells (DCs)-based vaccines are considered quite promising for cancer immunotherapy due to their exquisite potential induce tumor antigen-specific cytotoxic T cells. However, a lack of efficient protocols inducing immunogenic antigens limits the efficacy DC-based vaccines. Here, we found that plasma-activated medium (PAM) induces cell death (ICD) in but not an immortalized L929 line or human peripheral blood mononuclear PAM induced accumulation reactive oxygen species...
Purpose: Trichinella spiralis has evolved complex immunomodulatory mechanisms mediated by excretory-secretory products (ESL1) that enable its survival in the host. Consequently, ESL1 antigens display excellent potential for treating autoimmune diseases such as multiple sclerosis (MS). However, whether timely controlled delivery of vivo, natural infections, could enhance therapeutic MS is still unknown. Methods: To test this, we encapsulated into biodegradable poly (lactide-co-glycolic) acid...
Tungsten disulfide (WS2) nanoparticles have emerged in the biomedical field as potential theranostic agents due to their unique properties, including biocompatibility. However, impact on immune response remains unexplored. This study aimed evaluate effects of inorganic fullerene-like WS2 (IF-WS2) nanostructures human peripheral blood mononuclear cells (PBMCs) vitro. The investigated several parameters IF-WS2 nanoparticles. Cytotoxicity was assessed by measuring cell viability, apoptosis, and...
Biodentine, a tricalcium silicate cement, has emerged as retrograde root-end filling material to promote periapical lesion (PL) healing after apicoectomy. However, its underlying mechanisms remain unclear. This study tested the hypothesis that Biodentine stimulates osteogenic differentiation of mesenchymal stromal cells (MSCs) derived from PLs. The extract (B-Ex) was prepared following ISO 10993-12 by incubating polymerized in RPMI medium (0.2 g/mL) for three days at 37 °C. B-Ex,...
Immunoregulatory mechanisms within periapical lesions (PLs) are as of yet unexplored. Considering the crucial role DCs in controlling immune response PLs, immunomodulatory properties mesenchymal stem cells (MSCs), and colocalization MSCs situ, we wondered whether from PLs modulate development functions DCs. Using a model monocyte-derived DCs, showed that PL-MSCs inhibited differentiation via soluble factors, which IL-6 had minor effect, but did not impair their subsequent maturation induced...
Abstract COVID‐19 is characterized by a wide spectrum of disease severity, whose indicators and underlying mechanisms need to be identified. The role extracellular vesicles (EVs) in their biomarker potential, however, remains largely unknown. Aiming identify specific EV signatures patients with mild compared severe COVID‐19, we the composition 20 26 along 16 sex age‐matched healthy donors panel eight different antibodies imaging flow cytometry (IFCM). We correlated obtained data 37 clinical,...
Mesenchymal stem cells (MSCs) isolated from healthy dental tissues are being investigated as an alternative source of MSCs for the treatment damaged and inflammatory diseases. Here we whether periapical lesions (PL-MSCs) also possess multi-lineage differentiation capacity immunomodulatory properties.PL-MSCs, by collagenase/DNAse digestion surgically extracted PLs, were compared with non-inflamed pulp (DP-MSCs) follicle (DF-MSCs) their phenotype multi-potent potential. The anti-inflammatory...
Mesenchymal stem/stromal cells (MSCs, having both multi-potent differentiation potential and prominent immunomodulatory properties, are seen as a very powerful tool for the therapy of diseases characterized by tissue damage and/or unregulated immune responses. Dendritic (DCs key immunoregulatory at crossroads between immunity tolerance, able to fine-tune whole response via regulation adaptive immunity. Therefore, untangling complex interactions DCs MSCs is crucial understanding various...
The aim of this work was to study the cytotoxicity different fractions gold nanoparticles prepared by ultrasonic spray pyrolysis from scrap. target cells were rat thymocytes, as a type nonproliferating cells, and L929 mouse fibroblasts, continuous proliferating cells. Fractions 1 2, composed pure nanoparticles, determined scanning electron microscopy with combination energy dispersive X-ray analysis, nontoxic for but reduced moderately proliferative activity inhibitory effect fraction...
Pomegranate peel extract (PoPEx) has been shown to have antioxidant and anti-inflammatory properties, but its effect on the adaptive immune system not sufficiently investigated. In this study, treatment of human peripheral blood mononuclear cells (PBMC) with PoPEx (range 6.25-400 µg/mL) resulted in cytotoxicity at concentrations 100 µg/mL higher, due induction apoptosis oxidative stress, whereas autophagy was reduced. At non-cytotoxic concentrations, opposite these processes observed...