A5073990292- Immunotherapy and Immune Responses
- Thyroid Cancer Diagnosis and Treatment
- Advanced Cellulose Research Studies
- Neonatal Respiratory Health Research
- Graphene and Nanomaterials Applications
- Adenosine and Purinergic Signaling
- Polysaccharides and Plant Cell Walls
- Pleural and Pulmonary Diseases
- Bee Products Chemical Analysis
- Blood disorders and treatments
- Endodontics and Root Canal Treatments
- Immune Cell Function and Interaction
- Carbon and Quantum Dots Applications
- Nanoparticles: synthesis and applications
- Immune Response and Inflammation
- Advancements in Transdermal Drug Delivery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Autoimmune and Inflammatory Disorders
- Pomegranate: compositions and health benefits
- Cystic Fibrosis Research Advances
- Vitamin C and Antioxidants Research
- Salivary Gland Disorders and Functions
- Advanced Drug Delivery Systems
- Geochemistry and Elemental Analysis
- Polydiacetylene-based materials and applications
Military Medical Academy
2013-2024
University of East Sarajevo
2021-2023
University of Defence
2014-2022
University of Sarajevo
2022
University of Belgrade
2018
Erasmus MC
2014
University of Kragujevac
2013-2014
Abstract Cellulose nanofibrills (CNFs) are attractive biocompatible, natural nanomaterials for wide biomedical applications. However, the immunological mechanisms of CNFs have been poorly investigated. Considering that dendritic cells (DCs) key immune regulatory in response to nanomaterials, our aim was investigate a model DC-mediated response. We found non-toxic concentrations impaired differentiation and subsequent maturation human monocyte-derived (mo)-DCs. In co-culture with CD4 + T...
Background/Aim. Piclidenoson (CF101, IB-MECA), a selective agonist of the A3 adenosine receptor (A3AR), is used in clinical trials for treatment psoriasis. Emerging data from vitro and vivo studies suggest that piclidenoson possesses anti-inflammatory immunomodulatory properties, but its action on human peripheral blood mononuclear cells (PBMCs) remains unknown. The aim this study was to examine effects model phytohaemagglutinin (PHA)-stimulated PBMCs culture. Methods. Human were isolated...
COVID-19 clinically manifests from asymptomatic to the critical range.Immune response provokes pro-inflammatory interactions, which lead cytokines, reactive oxygen/nitrogen species, peptidases, and arachidonic acid metabolites enlargement activation of coagulation components.Matrix metalloproteinases (MMPs) contribute tissue destruction in development COVID-19.Due endothelial, systemic course disease, VEGF A participates actively development, while neurotrophic metabolic effects BDNF...
Our recent study has shown that pomegranate peel extract (PEx) showed significant immunomodulatory activity, which might be caused by ellagitannins. The aim of this work was to test the hypothesis ellagitannin components act synergistically in modulation cytokine production.Human peripheral blood mononuclear cells (PBMCs) from healthy donors were stimulated with phytohemagglutinin and treated different concentrations PEx or punicalagin (PG), punicalin (PN), ellagic acid (EA), alone their...
Cellulose nanofibrils (CNF) are attractive nanomaterials for various biomedical applications due to their excellent biocompatibility and biomimetic properties. However, immunoregulatory properties insufficiently investigated, especially in relation functionalization, which could cause problems during clinical application.Using a model of human dendritic cells (DC), have central role the regulation immune response, we investigated how differentially functionalized CNF, ie, native (n)...
Although promising for active immunization in cancer patients, dendritic cells (DCs) vaccines generated vitro display high inter-individual variability their immunogenicity, which mostly limits therapeutic efficacy. Gut microbiota composition is a key emerging factor affecting individuals' immune responses, but it unknown how affects the of donors' precursor to differentiate into immunogenic DCs vitro. By analyzing gut 14 healthy donors, along with phenotype and cytokines production by...
Dysfunction of neutrophils in patients with cystic fibrosis (CF) is best characterized bronchoalveolar lavage (BAL), whereas peripheral blood are less examined, and the results contradictory, especially younger populations. Therefore, this work aimed to study functional phenotypic changes circulating children CF. The included 19 CF (5-17 years) 14 corresponding age-matched healthy children. Isolated were cultured either alone or different stimuli. Several functions studied: apoptosis,...
Even though IL-6 is a key inflammatory cytokine in periapical lesions (PLs), its function stable disease unknown. Therefore, the aim of this study was to investigate following: first, ex vivo production by clinically different PLs; next, subsets immune cells expressing PLs according their status and finally, modulatory effect on T-cell cell cultures.Inflammatory were isolated from total 95 human PLs. Detection IL-6+ within myeloid lymphoid populations performed multicolour flow cytometry....
Purpose: Phosphonates, like 3-AminoPropylphosphonic Acid (ApA), possess a great potential for the therapy of bone tumours, and their delivery via cellulose nanocrystals (CNCs) seems promising approach increased efficacy in target tissues.However, immunological effects CNC-phosphonates have not been investigated thoroughly.The main aim was to examine how modification CNCs with phosphonate affects immunomodulatory properties human cells.Methods: Wood-based native (n) were modified oxidation...
Abstract The aim of this study was the evaluation 15 days dietary regimen depurinized (DP) milk (obtained using our patented technological procedures) or 1.5% fat UHT instead standard chow diet, on rat thymus and bone marrow MyD88/Akt/p38, NF-κB, caspase-1 endonuclease pathways, in relation to peripheral blood cell composition. To determine whether reduced mass is a consequence direct effect DP/UHT apoptosis thymocytes, vitro Annexin-V-FITC/PI assay performed. Significant decreases wet...
Sitagliptin, an anti-diabetic drug, is a dipeptidyl peptidase (DPP)-4/CD26 inhibitor with additional anti-inflammatory and immunomodulatory properties. In this study, we investigated for the first time effect of sitagliptin on differentiation functions human dendritic cells generated from monocytes (MoDCs) 4 days using standard GM-CSF/IL-4 procedure. LPS/IFN-γ treatment 24 h was used maturation induction MoDCs. Sitagliptin added at highest non-cytotoxic concentration (500 µg/mL) either...
Dapsone is a sulfone used in treating inflammatory skin conditions. Despite its widespread dermatological use, the pharmacological actions of dapsone remain poorly understood. Here, we examined how different aspects neutrophil functions are affected by dapsone. Peripheral blood neutrophils from healthy donors were stimulated with phorbol-12-myristate-13-acetate (PMA), N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), or calcium ionophore (CaI) primed cytokines prior to stimulation,...
Background/Aim. Mesenchymal stem cells (MSCs) have been shown to suppress immune and inflammatory reactions. However, it is not known whether MSCs from tissues, such as periapical lesions (PLs) similar effects. This question was addressed in this study which the aim examine capacity of PL-MSCs for modulating cytokine production by local cells. Methods. were isolated asymptomatic (as) symptomatic (sy) PLs. Their phenotype analyzed flow cytometry detecting MSC surface markers....
Event Abstract Back to Co-ligation of TLR3 and Dectin-1 agonists potentiates Th1 polarization capability on monocyte derived Langerhans cells in vitro Ivan Rajkovic1*, Ana Dragicevic2, Sasa Vasilijic1, 2, Dragana Vucevic1, Biljana Bozic3, Dusan Mihajlovic1, Milan Markovic1 Miodrag Colic1 1 Medical Faculty the Military Academy, University Defense, Serbia 2 Institute for Research, 3 Biology, Belgrade, Physiology Biochemistry, represent first dendritic cell subtype that encounters foreign...