A5069398620- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Origins and Evolution of Life
- Viral Infections and Immunology Research
Pennsylvania State University
2020-2024
Abstract Multivalent polyions can undergo complex coacervation, producing membraneless compartments that accumulate ribozymes and enhance catalysis, offering a mechanism for functional prebiotic compartmentalization in the origins of life. Here, we evaluate impact lower, more prebiotically-relevant, polyion multivalency on performance coacervates as compartments. Positively negatively charged homopeptides with 1–100 residues adenosine mono-, di-, triphosphate nucleotides are used model...
Compartmentalization of RNA in biopolymer-rich membraneless organelles is now understood to be pervasive and critical for the function extant biology has been proposed as a prebiotically plausible way accumulate RNA. However, compartment-RNA interactions that drive encapsulation have potential influence structure compartment- sequence-dependent ways. Here, we detail next-generation sequencing (NGS) experiments performed compartments called complex coacervates characterize fold many different...
Abstract Multivalent polyions can undergo complex coacervation, producing membraneless compartments that accumulate ribozymes and enhance catalysis, offering a mechanism for functional prebiotic compartmentalization in the origins of life. Here, we evaluated impact low, prebiotically-relevant polyion multivalency coacervate performance as compartments. As model polyions, used positively negatively charged homopeptides with one to 100 residues, adenosine mono-, di-, triphosphate nucleotides....
Abstract Twister ribozymes are an extensively studied class of nucleolytic RNAs. Thousands natural twisters have been proposed using sequence homology and structural descriptors. Yet, most these candidates not validated experimentally. To address this gap, we developed Cleavage High-Throughput Assay (CHiTA), a high-throughput pipeline utilizing massively parallel oligonucleotide synthesis next-generation sequencing to test putative en masse in scarless fashion. As proof principle, applied...
Compartmentalization of RNA in biopolymer-rich membraneless organelles is now understood to be pervasive and critical for the function extant biology has been proposed as a prebiotically-plausible way accumulate RNA. However, compartment-RNA interactions that drive encapsulation have potential influence structure compartment- sequence-dependent ways. Herein, we detail Next-Generation Sequencing (NGS) experiments performed first time compartments called complex coacervates characterize fold...
ABSTRACT Twister ribozymes are an extensively studied class of nucleolytic RNAs. Thousands natural twisters have been proposed using sequence homology and structural descriptors. Yet, most these candidates not validated experimentally. To address this gap, we developed CHiTA (Cleavage High-Throughput Assay), a high-throughput pipeline utilizing massively parallel oligonucleotide synthesis next-generation sequencing to test putative en masse in scarless fashion. As proof principle, applied...
Abstract The COVID-19 pandemic persists despite the development of effective vaccines. As such, it remains crucial to identify new targets for antiviral therapies. causative virus COVID-19, SARS-CoV-2, is a positive-sense RNA with structures that could serve as therapeutic targets. One such established function frameshift stimulatory element (FSE), which promotes programmed ribosomal frameshifting. To accelerate identification additional functional elements, we introduce novel computational...