A5020605092- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
University of California, Santa Cruz
2018-2024
The genome is packaged and organized in an ordered, nonrandom manner, specific chromatin segments contact nuclear substructures to mediate this organization. tRNA genes (tDNAs) are binding sites for transcription factors architectural proteins thought play important role the organization of genome. In study, we investigate roles tDNAs genomic chromosome function by editing a so that it lacked any tDNAs. Surprisingly our analyses tDNA-less show loss does not grossly affect architecture or...
Transcriptional silencing in Saccharomyces cerevisiae involves the generation of a chromatin state that stably represses transcription. Using multiple reporter assays, diverse set upstream activating sequence enhancers and core promoters were investigated for their susceptibility to silencing. We show heterochromatin silences only weak stress-induced regulatory elements but is unable repress housekeeping gene elements, partial repression these did not result bistable expression states....
Significance Gene silencing, once established, is stably maintained for several generations. Despite the high fidelity of inheritance silent state, individual components silenced chromatin are in constant flux. Models suggest that loci can tolerate fluctuations Sir proteins and histone acetylation levels, but level tolerance unknown. To understand quantitative relationships between H4K16 acetylation, proteins, we developed assays to quantitatively alter a mimic allele protein levels measure...
Abstract The genome is packaged and organized in an ordered, non-random manner specific chromatin segments contact nuclear substructures to mediate this organization. While transfer RNA genes (tDNAs) are essential for the generation of tRNAs, these loci also binding sites transcription factors architectural proteins thought play important role organization genome. In study, we investigate tDNAs genomic chromosome function by editing a so that it lacks any tDNAs. Surprisingly our analyses...
Abstract The interplay between nucleosomes and transcription factors leads to programs of gene expression. Transcriptional silencing involves the generation a chromatin state that represses is faithfully propagated through DNA replication cell division. Using multiple reporter assays, including directly visualizing in single cells, we investigated diverse set UAS enhancers core promoters for their susceptibility heterochromatic silencing. These results show heterochromatin only stably...
Abstract Gene silencing in budding yeast is mediated by Sir protein binding to unacetylated nucleosomes form a chromatin structure that inhibits transcription. This transcriptional characterized the high-fidelity transmission of silent state. Despite its relative stability, constituent parts state are constant flux giving rise model loci can tolerate such fluctuations without functional consequences. However, level tolerance unknown and we developed method measure threshold histone...