A5019993983- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Chromosomal and Genetic Variations
- Fungal Biology and Applications
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Trypanosoma species research and implications
- Protein Kinase Regulation and GTPase Signaling
- DNA and Nucleic Acid Chemistry
- Plant Molecular Biology Research
- Insect symbiosis and bacterial influences
- Plant and fungal interactions
- Antifungal resistance and susceptibility
- Genetically Modified Organisms Research
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Plant Pathogens and Fungal Diseases
- Glycosylation and Glycoproteins Research
- Toxoplasma gondii Research Studies
- Advanced biosensing and bioanalysis techniques
- Herpesvirus Infections and Treatments
- RNA modifications and cancer
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2025
Experimental Medicine and Biology Institute
2020-2023
Fundación Ciencias Exactas y Naturales
2007-2023
University of Buenos Aires
2008-2023
Universidad de la República
2023
Instituto de Investigaciones Biológicas Clemente Estable
2023
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2014-2020
National Institutes of Health
2014-2020
Adenosine triphosphate-dependent chromatin remodeling machines play a central role in gene regulation by manipulating structure. Most genes have nucleosome-depleted region at the promoter and an array of regularly spaced nucleosomes phased relative to transcription start site. In vitro, three known yeast nucleosome spacing enzymes (CHD1, ISW1 ISW2) form arrays with different spacing. We used genome-wide sequencing determine whether these space differently vivo. find that CHD1 compete set on...
Eukaryotic chromatin is composed of nucleosomes, which contain nearly two coils DNA wrapped around a central histone octamer. The octamer contains an H3-H4 tetramer and H2A-H2B dimers. Gene activation associated with disruption: wider nucleosome-depleted region (NDR) at the promoter reduced nucleosome occupancy over coding region. Here, we examine nature disrupted after induction, using MNase-seq to map nucleosomes subnucleosomes, refined high-resolution ChIP-seq method H4, H2B RNA...
DNA accessibility is thought to be of major importance in regulating gene expression. We test this hypothesis using a restriction enzyme as probe chromatin structure and proxy for transcription factors. measured the digestion rate fraction accessible at almost all genomic AluI sites budding yeast mouse liver nuclei. Hepatocyte more than DNA, consistent with longer linkers between nucleosomes, suggesting that nucleosome spacing determinant accessibility. varies from cell cell, such...
Most yeast genes have a nucleosome-depleted region (NDR) at the promoter and an array of regularly spaced nucleosomes phased relative to transcription start site. We examined interplay between RSC (a conserved essential SWI/SNF-type complex that determines NDR size) ISW1, CHD1, ISW2 nucleosome spacing enzymes in chromatin organization transcription, using isogenic strains lacking all combinations these enzymes. The contributions remodelers are largely combinatorial, distinct, nonredundant,...
Preparation of fecal samples for alpha-synuclein seed amplification assay.
Restricting the localization of histone H3 variant CENP-A (Cse4 in yeast, CID flies) to centromeres is essential for faithful chromosome segregation. Mislocalization leads chromosomal instability (CIN) fly and human cells. Overexpression mislocalization has been observed many cancers this correlates with increased invasiveness poor prognosis. Yet genes that regulate levels under physiological conditions have not defined. In study we used a genome-wide genetic screen identify required Cse4...
Eukaryotic chromosomal DNA is assembled into regularly spaced nucleosomes, which play a central role in gene regulation by determining accessibility of control regions. The nucleosome contains ∼147 bp wrapped ∼1.7 times around core histone octamer. linker histone, H1, binds both to the nucleosome, sealing coils, and between directing chromatin folding. Micrococcal nuclease (MNase) digests yield chromatosome, containing H1 ∼160 bp, then converts it particle, no H1. Sequencing nucleosomal...
ABSTRACT The cyclic AMP (cAMP)-dependent protein kinase A (PKA) signaling pathway plays a role in regulating development, growth, and virulence number of fungi. To determine whether PKA similar function zygomycete fungi, mutant Mucor circinelloides was generated that lacks pkaR1 , one the regulatory subunits PKA. showed reduction growth alterations germination rates, cell volume, germ tube length, asexual sporulation. lack gene resulted highly decreased, but not null, cAMP binding activity...
Subtelomeres (ST) are chromosome regions that separate telomeres from euchromatin and play relevant roles in various biological processes of the cell. While their functions conserved, ST structure genetic compositions unique to each species. This study aims identify characterize subtelomeric 13 Toxoplasma gondii chromosomes Me49 strain. Here, STs were defined at ends based on poor gene density. The length ranges 8.1 232.4 kbp, with a density 0.049 genes/kbp, lower than genome (0.15 kbp)....
ABSTRACT The protein kinase A (PKA) signaling pathway plays a role in regulating growth and differentiation the dimorphic fungus Mucor circinelloides . PKA holoenzyme is comprised of two catalytic (C) regulatory (R) subunits. In M. , four genes encode PKAR1, PKAR2, PKAR3, PKAR4 isoforms R We have constructed null mutants demonstrate that each isoform has different differentiation. most striking finding pkaR4 an essential gene, because only heterokaryons were obtained knockout experiments....
The vast majority of methods available to characterize genome-wide chromatin structure exploit differences in DNA accessibility nucleases or chemical crosslinking. We developed a novel method gauge histone protein surfaces within nucleosomes by assessing reactivity engineered cysteine residues with thiol-specific reagent, biotin-maleimide (BM).Yeast nuclei were obtained from cells expressing the mutant H2B S116C, which resides near center external flat surface nucleosome. BM modification...
The evolutionarily conserved centromeric histone H3 variant (Cse4 in budding yeast, CENP-A humans) is essential for faithful chromosome segregation. Mislocalization of to non-centromeric chromatin contributes chromosomal instability (CIN) fly, and human cells highly expressed mislocalized cancers. Defining mechanisms that prevent mislocalization an area active investigation. Ubiquitin-mediated proteolysis overexpressed Cse4 (GALCSE4) by E3 ubiquitin ligases such as Psh1 prevents Cse4, psh1 Δ...
The genome is packaged and organized in an ordered, nonrandom manner, specific chromatin segments contact nuclear substructures to mediate this organization. tRNA genes (tDNAs) are binding sites for transcription factors architectural proteins thought play important role the organization of genome. In study, we investigate roles tDNAs genomic chromosome function by editing a so that it lacked any tDNAs. Surprisingly our analyses tDNA-less show loss does not grossly affect architecture or...
ABSTRACT DNA accessibility is thought to be of major importance in regulating gene expression. We test this hypothesis using a restriction enzyme as probe chromatin structure and proxy for transcription factors. measured the digestion rate fraction accessible at all genomic Alu I sites budding yeast mouse liver nuclei. Hepatocyte more than DNA, consistent with longer linkers between nucleosomes, indicating that nucleosome spacing determinant accessibility. varies from cell cell, such...
Abstract The genome is packaged and organized in an ordered, non-random manner specific chromatin segments contact nuclear substructures to mediate this organization. While transfer RNA genes (tDNAs) are essential for the generation of tRNAs, these loci also binding sites transcription factors architectural proteins thought play important role organization genome. In study, we investigate tDNAs genomic chromosome function by editing a so that it lacks any tDNAs. Surprisingly our analyses...