A5084510737- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Plant Molecular Biology Research
- Genomic variations and chromosomal abnormalities
- CRISPR and Genetic Engineering
- Plant Genetic and Mutation Studies
- Genetics, Aging, and Longevity in Model Organisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ubiquitin and proteasome pathways
- Plant Virus Research Studies
- Pluripotent Stem Cells Research
- DNA and Nucleic Acid Chemistry
- Prenatal Screening and Diagnostics
Rutgers, The State University of New Jersey
2015-2024
University of Pennsylvania
2017-2023
Marine Biological Laboratory
2017
University of Chicago
2017
Sister centromere fusion is a process unique to meiosis that promotes co-orientation of the sister kinetochores, ensuring they attach microtubules from same pole during metaphase I. We have found kinetochore protein SPC105R/KNL1 and Protein Phosphatase 1 (PP1-87B) regulate in Drosophila oocytes. The analysis these two proteins, however, has shown independent mechanisms maintain fusion. Maintenance by SPC105R depends on Separase, suggesting cohesin proteins must be maintained at core...
The reductional division of meiosis I requires the separation chromosome pairs towards opposite poles. We have previously implicated outer kinetochore protein SPC105R/KNL1 in driving segregation through lateral attachments to microtubules and coorientation sister centromeres. To identify domains SPC105R that are critical for meiotic segregation, an RNAi-resistant gene expression system was developed. found C-terminal domain (aa 1284-1960) is necessary sufficient recruiting NDC80 building...
Oocytes segregate chromosomes in the absence of centrosomes. In this situation, direct spindle assembly. It is still unclear system which factors are required for homologous chromosome bi-orientation and The Drosophila kinesin-6 protein Subito, although nonessential mitotic assembly, to organize a bipolar meiotic oocytes. Along with chromosomal passenger complex (CPC), Subito an important part metaphase I central spindle. study we have conducted genetic screens identify genes that interact...
Meiosis in female oocytes lacks centrosomes, the microtubule-organizing centers. In Drosophila oocytes, meiotic spindle assembly depends on chromosomal passenger complex (CPC). To investigate mechanisms that regulate Aurora B activity, we examined role of protein phosphatase 2A (PP2A) oocyte meiosis. We found both forms PP2A, B55 and B56, antagonize function, suggesting a balance between PP2A activity maintains during meiosis I. PP2A-B56, which has subunit encoded by two partially redundant...
Bipolar spindle assembly occurs in the absence of centrosomes oocytes most organisms. In Drosophila oocytes, we have proposed that kinesin 6 Subito, a MKLP-2 homolog, is required for establishing bipolarity and chromosome biorientation by assembling robust central during prometaphase I. Although functions conserved motor domains kinesins well studied, less known about contribution poorly N- C- terminal to function. this study, investigated these meiosis early embryonic development. We found...
The reductional division of meiosis I requires the separation chromosome pairs towards opposite poles. We have previously implicated outer kinetochore protein SPC105R/KNL1 in driving segregation through lateral attachments to microtubules and co-orientation sister centromeres. To identify domains SPC105R that are critical for meiotic segregation, an RNAi-resistant gene expression system was developed. found SPC105R's C-terminal domain (aa 1284-1960) is necessary sufficient recruiting NDC80...
Centromeres direct genetic inheritance but are not themselves genetically encoded. Instead, centromeres defined epigenetically by the presence of a histone H3 variant, CENP-A. In cultured somatic cells, an established paradigm cell cycle-coupled propagation maintains centromere identity: CENP-A is partitioned between sisters during replication and replenished new assembly, which restricted to G1. The mammalian female germline challenges this model because cycle arrest pre-meiotic S-phase...
Centromeres direct genetic inheritance but are not themselves genetically encoded. Instead, centromeres defined epigenetically by the presence of a histone H3 variant, CENP-A
Accurate chromosome segregation is critical for the formation of haploid gametes, and therefore healthy offspring. Errors in result aneuploidy, which increases exponentially with maternal age (Hassold Hunt, 2001). Maternally aged mouse oocytes were recently found to exhibit premature sister chromatid separation due reduced levels Securin, an essential regulator cohesion (Nabti et al, 2017). This article protected by copyright. All rights reserved
Abstract Sister centromere fusion is a process unique to meiosis that promotes co-orientation of the sister kinetochores, ensuring they attach microtubules from same pole. We have found kinetochore protein SPC105R/KNL1 and Protein Phosphatase 1 (PP1-87B) are required for this process. The analysis these two proteins, however, has shown independent mechanisms maintain during I in Drosophila oocytes. Double depletion experiments demonstrated precocious separation centromeres Spc105R RNAi...
Abstract Meiosis in female oocytes lack centrosomes, the major microtubule-organizing center, which makes them especially vulnerable to aneuploidy. In acentrosomal of Drosophila , meiotic spindle assembly depends on chromosomal passenger complex (CPC). Aurora B is catalytic component CPC while remaining subunits regulate its localization. Using an inhibitor activity, Binucleine 2, we found that continuous activity required maintain oocyte during meiosis I, and this antagonized by...
Summary The CENP-A histone variant epigenetically defines centromeres, where its levels and locations are precisely maintained through mitotic cell divisions. However, differences in centromere propagation soma versus female/male germline remains poorly understood. Here, we generated C enpa mScarlet mice followed dynamics gametes, zygotes, embryos. We found that, unlike somatic cells, progenitor female male germ cells carry high centromeric that decrease upon terminal differentiation....