A5030820459- Chemical Reactions and Isotopes
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Fluorine in Organic Chemistry
- Carbon dioxide utilization in catalysis
- Graphene research and applications
- CO2 Reduction Techniques and Catalysts
- Ammonia Synthesis and Nitrogen Reduction
- Graphene and Nanomaterials Applications
- Ferrocene Chemistry and Applications
- Crystallography and molecular interactions
- Amino Acid Enzymes and Metabolism
- Carbon and Quantum Dots Applications
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Medical Imaging Techniques and Applications
- Synthesis and Biological Evaluation
- Protein Degradation and Inhibitors
- Cyclopropane Reaction Mechanisms
- Coordination Chemistry and Organometallics
- Synthesis of β-Lactam Compounds
- Catalytic Alkyne Reactions
- Monoclonal and Polyclonal Antibodies Research
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2017-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
CEA Paris-Saclay
2017-2024
Université Paris-Saclay
2017-2024
Technologies pour la Santé
2020-2024
Laboratoire de Chimie Organique
2017-2023
CEA Paris-Saclay - Etablissement de Saclay
2023
Unité de Technologies Chimiques et Biologiques pour la Santé
2021-2023
Institut de Biologie et Technologies
2022
Direction de la Recherche Fondamentale
2020-2021
Two-dimensional (2D) materials have attracted tremendous interest ever since the isolation of atomically thin sheets graphene in 2004 due to specific and versatile properties these materials. However, increasing production use 2D necessitate a thorough evaluation potential impact on human health environment. Furthermore, harmonized test protocols are needed with which assess safety The Graphene Flagship project (2013-2023), funded by European Commission, addressed identification possible...
Abstract We report the discovery of a new bioorthogonal click‐and‐release reaction involving iminosydnones and strained alkynes. This transformation leads to two products resulting from ligation fragmentation under physiological conditions. Optimized were successfully used design innovative cleavable linkers for protein modification, thus opening up areas in fields drug release target‐fishing applications. technology offers possibility exchanging tags on proteins functionalized cyclooctynes mild
An integrated catalytic decarboxylation/carboxylation for accessing isotopically labeled carboxylic acids with 13CO2 or 14CO2 is described. The method shows a wide scope under mild conditions, even in the context of late-stage functionalization, and does not require stoichiometric organometallics, thus complementing existing carbon-labeling techniques en route to acids.
A photocatalytic approach for carbon isotope exchange is reported. Utilizing [13C]CO2 and [14C]CO2 as primary C1 sources, this protocol allows the insertion of desired into phenyl acetic acids without need structural modifications or prefunctionalization in one single step. The exceptionally mild conditions required traceless transformation are stark contrast with those previous methods requiring use harsh thermal conditions.
Harvesting sunlight to drive carbon dioxide (CO2) valorisation represents an ideal concept support a sustainable and carbon-neutral economy. While the photochemical reduction of CO2 monoxide (CO) has emerged as hot research topic, full CO2-to-CO conversion remains often-overlooked criterion that prevents productive direct CO into high-value-added chemicals. Herein, we report photocatalytic process unlocks fast (<10 min) its straightforward human health related field radiochemistry with...
In this work, we explore a nickel-catalyzed reversible carbon–sulfur (C–S) bond activation strategy to achieve selective sulfur isotope exchange. Isotopes are at the foundation of applications in life science, such as nuclear imaging, and essential tools for determination pharmacokinetic dynamic profiles new pharmaceuticals. However, insertion an into organic molecule remains challenging, current technologies element-specific. Despite ubiquitous presence many biologically active molecules,...
Copper-catalyzed and copper-free sydnone-alkyne cycloaddition reactions have emerged as complementary click tools for chemical biology but their use in bioorthogonal labeling is still its infancy. Herein, combinations of alkynes coumarin-sydnones were screened ability to generate pyrazole products displaying strong fluoroscence enhancement compared reactants. One sydnone was identified a particularly suitable new turn-on probe protein labeling.
Examples of organometallic compounds as nucleoside analogues are rare within the field medicinal bioorganometallic chemistry. We report on synthesis and properties two chiral ferrocene derivatives containing a nucleobase hydroxyalkyl group. These so-called ferronucleosides show promising anticancer activity, with cytostatic studies five different cancer cell lines indicating that both functional groups required for optimal activity.
The design, synthesis and electrochemical behaviour of an oligomer consisting linked thymine-functionalised ferrocene units are reported, which, as a so-called form nucleic acid (FcNA), acts structural mimic DNA.
Abstract We report the discovery of a new bioorthogonal click‐and‐release reaction involving iminosydnones and strained alkynes. This transformation leads to two products resulting from ligation fragmentation under physiological conditions. Optimized were successfully used design innovative cleavable linkers for protein modification, thus opening up areas in fields drug release target‐fishing applications. technology offers possibility exchanging tags on proteins functionalized cyclooctynes mild
We report the synthesis and use of sydnone-based profluorophores as tools for imaging applications. These new probes display exquisite reactivity towards strain promoted cycloaddition reactions with cycloalkynes allowing fast, efficient selective labeling in biological media. Styryl-pyridinium sydnone were found particularly interesting click to proceed selectively inside cells.
A general approach to label urea derivatives with all carbon isotopes (<sup>11</sup>C, <sup>13</sup>C and <sup>14</sup>C) is reported.
Amide isotope labeling is a crucial tool in drug discovery and development. However, current methods label the desired peptide bond multi-step fashion. This study presents novel strategies for amide exchange through transamidation metathesis. By optimizing reaction conditions exploring substrate scope, we achieved direct of both amine carbonyl moieties without need precursors preparation. These findings pave way sustainable technologies. Furthermore, preliminary examples isotope-containing...
A general procedure for the late-stage [11C], [13C] and [14C]carbon isotope labeling of cyclic carbamates is reported. This protocol allows incorporation carbon dioxide, primary source carbon-14 carbon-11 radioisotopes, in a direct, cost-effective sustainable manner. disconnection/reconnection strategy, involving ring opening/isotopic closure, was also implemented.
The incorporation of carbon-14 allows tracking organic molecules and provides vital knowledge on their fate. This information is critical in pharmaceutical development, crop science, human food safety evaluation. Herein, a transition-metal-catalyzed procedure enabling carbon isotope exchange aromatic nitriles described. By utilizing the radiolabeled precursor Zn([14C]CN)2, this protocol insertion desired tag without need for structural modifications, single step. reducing synthetic costs...
The reactivity of functionalized ynamides and arylynamines with tetracyanoethylene at room temperature was evaluated. In most cases, the corresponding 1,1,4,4-tetracyanobutadienes (TCBDs) were obtained in good to excellent yields through a [2+2]-cycloaddition/[2+2]-retro-electrocyclization sequence. influence diverse functional groups on yield reaction investigated, particular concerning multiple ynamides. These TCBDs characterized by various spectroscopic techniques electrochemistry X-ray...
Research on graphene based nanomaterials has flourished in the last decade due their unique properties and emerging socio-economic impact. In context of potential exploitation for biomedical applications, there is a growing need development more efficient imaging techniques to track fate these materials. Herein we propose first correlative approach combination radioimaging mass spectrometry detection Graphene Oxide (GO) labelled with carbon-14 mice. this study, 14C-graphene oxide nanoribbons...
In preclinical models, the development and optimization of protein-drug conjugates require accurate determination plasma tissue profiles both protein its conjugated drug. To this aim, we developed a bioanalytical strategy based on dual radiolabeling ex vivo digital imaging. By combining enzymatic chemical reactions, obtained homogeneous dual-labeled anti-MMP-14 Fabs (antigen-binding fragments) to monomethyl auristatin E where scaffold was labeled with carbon-14 (14C) drug tritium (3H). These...
A convenient and effective synthetic access to chelating azides was designed enabling the preparation of efficient clickable fluorescent derivatives. The comparison reactivity these regular showcased striking superiority such derivatives for labeling applications.
Summary Early detection of expanded‐spectrum cephalosporin (ESC) resistance is essential not only for an effective therapy but also the prompt implementation infection control measures to prevent dissemination in hospital. We have developed and validated a lateral flow immunoassay (LFIA), called LFIA‐CTX test, ESC (cefotaxime) hydrolytic activity based on structural discrimination between intact antibiotic its hydrolysed product. A single bacterial colony was suspended extraction buffer...
Research on graphene-based nanomaterials has experienced exponential growth in the last few decades, driven by their unique properties and future potential impact our everyday life. With increasing production commercialization of these materials, there is significant interest understanding fate vivo. Herein, we investigated distribution 14C-few-layer graphene (14C-FLG) flakes (lat. dim. ∼ 500 nm) mice over a period one year. Furthermore, compared effects repeated low-dose acute high-dose...
Four organometallic nucleobases have been prepared and characterized, each consisting of a disubstituted ferrocene unit connected through either conjugated or saturated linker group to adenine thymine nucleobases. Their assembly behavior has studied in the solid state via X-ray crystallography, revealing intermolecular H-bonded arrays. The electrode potentials DCM are strongly dependent upon nature between nucleobase.
Graphene-based nanoparticles are continuously being developed for biomedical applications, and their use raises concerns about environmental biological impact. In the literature, some imaging techniques based on fluorescence radioimaging have been used to explore fate in vivo. Here, we report of label-free mass spectrometry (MSI) graphene oxide (GO) reduced (rGO) analyses rodent tissues. Thereby, extend previous work by focusing practical questions obtain reliable meaningful images. Specific...