Root bark of mulberry (Morus alba L.) has been used in herbal medicine as anti-phlogistic, liver protective, kidney hypotensive, diuretic, anti-cough and analgesic agent. However, the anti-cancer activity potential mechanisms root have not elucidated. We performed vitro study to investigate whether extract (MRBE) shows anti-inflammatory activity. In activity, NO was measured using griess method. iNOS proteins regulating NF-κB ERK1/2 signaling were analyzed by Western blot. cell growth MTT...

Ginger leaf (GL) has long been used as a vegetable, tea and herbal medicine. However, its pharmacological properties are still poorly understood. Thus, we performed in vitro studies to evaluate anti-cancer of ginger then elucidate the potential mechanisms involved. Cell viability was measured by MTT assay. ATF3 expression level evaluated Western blot or RT-PCR transcriptional activity determined using dual-luciferase assay kit after transfection promoter constructs. In addition,...

Naringenin (NAR) as one of the flavonoids observed in grapefruit has been reported to exhibit an anti-cancer activity. However, more detailed mechanism by which NAR exerts properties still remains unanswered. Thus, this study, we have shown that down-regulates level cyclin D1 human colorectal cancer cell lines, HCT116 and SW480. inhibited proliferation SW480 cells decreased protein. Inhibition proteasomal degradation MG132 blocked NAR-mediated downregulation half-life was treated with NAR....

Naringenin (NAR) as one of the flavonoidsobserved in grapefruit has been reported to exhibit an anti-cancer activity. Activating transcription factor 3 (ATF3) is associated with apoptosis human colon cancer cells. This study was performed investigate molecular mechanism by which NAR stimulates ATF3 expression and reduced cell viability induced overexpression increased NAR-mediated cleaved PARP, while knockdown attenuated cleavage PARP NAR. both protein mRNA level, luciferase activity...

In this study, we investigated the immune-enhancing activity of water extracts from Hydrangea macrophylla subsp. serrata (WE-HML). WE-HML increased cell viability and production immunomodulators, which contributed to activating phagocytic in RAW264.7 cells. Inhibition JNK NF-κB reduced immunomodulators by WE-HML. ROS inhibition suppressed activation signaling TLR4 attenuated immunosuppressed mouse model, spleen index, levels cytokines, numbers white blood cells, lymphocytes, neutrophils....

The rhizome of Cnidium officinale Makino (COM) is known for its medicinal properties due to pharmacological activity. Typically, it thermally dried after harvesting before being processed into suitable final products. However, because thermal drying affects the levels thermolabile compounds in COM rhizomes, conditions are necessary ensure quality product. In this study, we evaluated effects temperatures (30-70°C) on and antioxidant rhizomes. highest concentrations key compounds, such as...

Phlorofucofuroeckol A (PFF-A), one of the phlorotannins found in brown algae, has been reported to exert anti-cancer property. However, molecular mechanism for effect PFF-A not known. Activating transcription factor 3 (ATF3) be associated with apoptosis colorectal cancer. The present study was performed investigate by which stimulates ATF3 expression and human cancer cells. decreased cell viability through increased regulating transcriptional activity. responsible cis-element activation cAMP...

Abstract Background Heracleum moellendorffii roots (HM-R) have been long treated for inflammatory diseases such as arthritis, backache and fever. However, an anti-inflammatory effect the specific mechanism of HM-R were not yet clear. In this study, we first time explored HM-R. Methods The cytotoxicity against RAW264.7 cells was evaluated using MTT assay. inhibition NO PGE 2 production by Griess reagent Prostaglandin E ELISA Kit, respectively. changes in mRNA or protein level following...

This study was conducted to compare the synbiotic activity between Corni fructus (C. fructus) and Limosilactobacillus reuteri (L. reuteri) on dextran sulfate sodium (DSS)-induced colitis cognitive dysfunction in C57BL/6 mice. C. (as prebiotics, PRE), L. probiotics, PRO), synbiotics a mixture of fructus, SYN) were fed mice for 3 weeks. Consumption PRE, PRO, SYN ameliorated symptoms body weight, large intestinal length, serum albumin level. Moreover, showed synergistic effect permeability...

Silymarin from milk thistle (Silybum marianum) has been reported to show an anti-cancer activity.In previous study, we that silymarin induces cyclin D1 proteasomal degradation through NF-κB-mediated threonine-286 phosphorylation.However, mechanism for the inhibition of Wnt signaling by still remains unanswered.Thus, investigated whether affects in human colorectal cancer cells elucidate additional silymarin.Transient transfection with a TOP and FOP FLASH luciferase construct indicated...

Abstract Heracleum moellendorffii ( H. ) is a family of Umbelliferae and has long been used for food medicinal purposes. However, the immune‐enhancing activity not studied. Thus, we evaluated in vitro through macrophage activation using RAW264.7 cells. Root extracts (HMR) increased production immunomodulators such as NO, iNOS, IL‐1β, IL‐6 IL‐12, TNF‐α, MCP‐1 activated phagocytosis Inhibition TLR2 TLR4 reduced induced by HMR. MAPK signaling attenuated HMR, but inhibitions NF‐κB or PI3K/AKT...

A cold storage system is useful for maintaining the quality of hardy kiwifruit. However, extended periods inevitably result in stress, leading to lower fruit marketability; severity chilling injury depends on types and cultivars. In this study, impact conditions physicochemical properties antioxidant capacity two phenotypically different kiwifruit cultivars-'Cheongsan' (large type) 'Daebo' (small type)-stored at low (L; 3 °C, relative humidity [RH]; 85-90%) moderate-low (ML; 5 RH;...

Sageretia thea (S. thea) commonly known as Chinese sweet plum or bird has been used for treating hepatitis and fevers in Korea China. S. reported to exert anti-oxidant, anticancer anti-human immunodeficiency virus activity. However, there is little study on the anti-inflammatory activity of thea. Thus, we evaluated effect extracts leaves (ST-L) branches (ST-B) from LPS-stimulated RAW264.7 cells. ST-L ST-B significantly inhibited production pro-inflammatory mediators such NO, iNOS, COX-2,...

Shiitake mushroom (Lentinula edodes) hold high nutritional and medicinal value as they contain an abundance of health-promoting compounds. However, the effect long-term postharvest storage on variation in levels compounds has not been extensively studied. In this study, we investigated changes phenolic compounds, antioxidants, eritadenine, ergothioneine shiitake mushrooms stored at three different temperatures (1, 3, 5 °C) for 4 weeks. Compared to lower temperatures, those °C exhibited a...

Recently, Abeliophyllum distichum Nakai (A. distichum) has been reported to exert the inhibitory effect on angiotensin converting enzyme. However, no specific pharmacological effects from A. have described. We performed in vitro study evaluate anti-cancer properties of and then elucidate potential mechanisms. Cell viability was measured by MTT assay. ATF3 expression level evaluated Western blot or RT-PCR transcriptional activity determined using a dual-luciferase assay kit after transfection...

Honeyberry (Lonicera caerulea) has long been used as a traditional medicine in China, Japan and northern Russia. Functional studies of honeyberry have mainly focused on the fruits, which reported to exert various pharmacological activities, including anti‑inflammatory activity, with limited or no other parts plant, such leaves branches. In present study, effects extracts (HBL), branches (HBB) fruit (HBF) plant were evaluated lipopolysaccharide (LPS)‑stimulated RAW264.7 cells. HBL HBB...

Abstract As the national flower of Korea, Hibiscus syriacus L. (Rose Sharon) is symbolic in its abundance and a prominent feature Korean culture. H. has played an important role not only as ornamental plant but also essential ingredient folk remedies through various parts. This study aimed to characterize nutritional biochemical composition each unit “Wonhwa.” The units are namely: petals, leaves, roots, sprouts from seeds. According results produced, had highest content amino acids fatty...

Sageretia thea (S. thea) has been used as the medicinal plant for treating hepatitis and fevers in Korea China. Recently, anticancer activity of S. reported, but potential mechanism anti-cancer property is still insufficient. Thus, we evaluated whether extracts from leaves (STL) branches (STB) exert elucidated its SW480 cells.MTT assay was performed measuring cell viability. Western blot RT-PCR were analyzing level protein mRNA, respectively.Treatment STL or STB decreased viability induced...