A5021332157- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Chemical synthesis and alkaloids
- Cellular Mechanics and Interactions
- Carbohydrate Chemistry and Synthesis
- Molecular spectroscopy and chirality
- RNA Research and Splicing
- Cholinesterase and Neurodegenerative Diseases
- Nuclear Structure and Function
- Mitochondrial Function and Pathology
- Sirtuins and Resveratrol in Medicine
- Synthetic Organic Chemistry Methods
- Cell death mechanisms and regulation
- Axial and Atropisomeric Chirality Synthesis
- Alzheimer's disease research and treatments
- Milk Quality and Mastitis in Dairy Cows
- S100 Proteins and Annexins
- Insect and Pesticide Research
- Cancer, Hypoxia, and Metabolism
- Insect Pheromone Research and Control
- Asymmetric Synthesis and Catalysis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ubiquitin and proteasome pathways
- FOXO transcription factor regulation
Showa University
2015-2025
Showa Pharmaceutical University
2020-2025
The Japanese Society of Gastroenterological Surgery
2024
Iwate Medical University
2024
Onomichi General Hospital
2024
Hiroshima University
2024
Cell Biotech (South Korea)
2015-2019
Fujita Health University
2019
Cliniques Universitaires Saint-Luc
2019
University of Tsukuba
2006
Abstract Although the causal relationship between chronic inflammation and carcinogenesis has long been discussed, molecular basis of relation is poorly understood. In present study, we focused on reactive oxygen species (ROS) their signals under inflammatory conditions leading to epithelial cells found that repeated treatment with a low dose H2O2 (0.2 mmol/L) for periods 2 4 days caused phenotypic conversion mouse NMuMG mammary from fibroblast-like as in malignant transformation. The...
The role of the electron transport chain (ETC) in cell proliferation control beyond its crucial function supporting ATP generation has recently emerged. In this study, we found that, among four ETC complexes, complex I (CI)‐mediated NAD + regeneration is important for cancer proliferation. cells, a decrease CI activity by RNA interference (RNAi) against NADH:ubiquinone oxidoreductase core subunit V1 ( NDUFV1 ) arrested cycle at G 1 /S phase, accompanying upregulation p21 Cip1...
hic-5 was originally isolated as an H(2)O(2)-inducible cDNA clone whose product normally found at focal adhesions. In this study, we that Hic-5 accumulated in the nucleus response to oxidants such H(2)O(2). Other adhesion proteins including paxillin, most homologous Hic-5, remained cytoplasm. Mutation analyses revealed C- and N-terminal halves of contributed its nuclear localization a positive negative manner, respectively. After finding leptomycin B (LMB), inhibitor export signal (NES),...
Changes in the activities of serum cytokines and acute phase response were observed dairy cows with naturally occurring coliform mastitis. Seven severe mastitis showed systemic mammary inflammatory throughout observation period, 11 mild recovered able to be milked within 3 days onset Serum interleukin (IL)-I tumor necrosis factor (TNF) higher group than at first appearance symptoms. Elevated IL-1 activity was evident period. alpha-1-acidglycoprotein (alpha1AG) concentration began rise...
Abstract Anchorage dependence of cellular growth and survival prevents inappropriate cell or in ectopic environments, serves as a potential barrier to metastasis cancer cells. Therefore, obtaining better understanding anchorage-dependent responses normal cells is the first step understand impede anchorage independence finally eradicate during metastasis. Anoikis, type apoptosis specifically induced by lack appropriate cell-extracellular matrix adhesion, has been established dominant response...
Mitochondria are considered to play an important role in oxidative stress response since they a source of reactive oxygen species and also targeted by these species. This study examined the mitochondrial conditions cells epithelial origin that were exposed H2O2 found decline membrane potential along with specific loss UQCRC1, sub-unit complex III, suggesting dysfunction occurs upon exposure stress. observation led hypothesis certain cellular responses occurred because dysfunction. When...
Silver is a transition metal that known to be less toxic than platinum. However, only few studies have reported the anticancer effects of some silver complexes and their possibility as an alternative platinum complex. This study investigated thiosulfate complex (STS), [Ag(S2O3)2]3-, consisting sodium thiosulfate.In vitro cytotoxic activity STS was comparatively in human cancer cell lines (K562 MCF-7) normal cells (mesenchymal stem mammary epithelial cells). For its effects, cycle, mode...
Aggressive cancers, such as triple-negative breast cancer (TNBC), are mostly fatal because of their potential to metastasize distant organs. Cancer cells acquire various abilities metastasize, including resistance anoikis, an apoptotic cell death induced by loss anchorage the extracellular matrix. Transcriptional coactivator with PDZ binding motif (TAZ) and Yes-associated protein (YAP), downstream effectors Hippo pathway, regulate cell- tissue-level architectures responding mechanical...
Mitochondria are multifunctional organelles; they have been implicated in various aspects of tumorigenesis. In this study, we investigated a novel role the basal electron transport chain (ETC) activity cell proliferation by inhibiting mitochondrial replication and transcription (mtR/T) using pharmacological genetic interventions, which depleted DNA/RNA, thereby inducing ETC deficiency. Interestingly, mtR/T inhibition did not decrease ATP levels despite deficiency different types, including...
The LIM protein Hic-5 is a focal adhesion shuttling in and out of the nucleus through redox-sensitive nuclear export signal, unlike other proteins including paxillin, most homologous to Hic-5, it accumulates under oxidative conditions participates transcription c-fos p21(Cip1) genes. Here, we examined roles interacting partners kinase (FAK) tyrosine phosphatase PEST (PTP-PEST), translocation found that they were inhibitory. Interestingly, interaction with FAK was regulated by specific...
Hic-5 is a focal adhesion LIM protein serving as scaffold in integrin signaling. The comprises four LD domains its N-terminal half and C-terminal with nuclear export signal LD3 shuttled between the cytoplasmic compartments. In this study, immunoprecipitation <i>in vitro</i> cross-linking experiments showed that homo-oligomerized through most domain, LIM4. Strikingly, paxillin, homologous to Hic-5, did not show capability. Gel filtration analysis also revealed differs from paxillin it has...
Anchorage dependence of cell growth and survival is a critical trait that distinguishes nontransformed cells from transformed cells. We demonstrate anchorage determined by anchorage-dependent nuclear retention cyclin D1, which regulated the focal adhesion protein, Hic-5, whose CRM1-dependent export counteracts D1. An adaptor PINCH, interacts with D1 Hic-5 potentially serves as an interface for competition between CRM1. In nonadherent cells, redox-sensitive, was interrupted due to elevated...
HIC-5 is a multidomain LIM protein homologous to paxillin that serves as molecular scaffold at focal adhesions and in the nucleus. It forms mobile units with LIM-only proteins, PINCH, CRP2 translocates out of nucleus via nuclear export signal (NES). Of note, NES distinctive its sensitivity cellular redox state. Recently, have been suggested be involved regulation anchorage dependence cell growth. On loss adhesion, an increase reactive oxygen species cells modifies stops shuttling, which...
In most human cancers, somatic mutations have been identified in the mtDNA; however, their significance remains unclear. We recently discovered that NMuMG mouse mammary epithelial cells, when deprived of mitochondria or following inhibition respiratory activity, undergo morphological disruption accompanied with irregular edging E-cadherin, appearance actin stress fibers, and an altered gene expression profile. this study, using mtDNA-less pseudo ρ0 cells obtained from we examined roles two...
Abstract
Silibinin (Sib), one of the main components milk thistle extract, has attracted considerable attention because its various biological activities, which include antioxidant activity and potential effects in diabetes Alzheimer's disease (AD). In a previous study, we synthesized catechin analogues by constraining geometries (+)-catechin (-)-epicatechin. The constrained exhibited enhanced bioactivities, with only major difference between two being their three-dimensional structures. geometry...