Abstract Purpose The aim of the underlying study was to present a new surgical method in PreserFlo MicroShunt surgery for glaucoma. A removable polyamide suture placed into lumen during implantation prevent early postoperative hypotony. Methods Thirty-one patients undergoing stand-alone glaucoma with and an intraluminal occlusion were retrospectively reviewed compared control group without occlusion. Inclusion criteria diagnosis primary open-angle or secondary due pseudoexfoliation pigment...

Abstract (Deutsch) Hintergrund: Ziel dieser Studie war es erstmalig in Deutschland die Akzeptanz von innovativen Perimetriemethoden, wie Virtual Reality-Brillenperimetrie (VRP) und Tablet-basierte Perimetrie (TBP) im Vergleich zur konventionelle Halbkugelperimetrie (HKP) bei Glaukompatienten zu untersuchen. Patienten Methoden: 204 wurden mit dem 30-2 TOP Programm (Octopus, Haag-Streit) untersucht anschließend einer der beiden neuen Gruppe 1 (VRP; n=101) 2 (TBP mittels Melbourne Rapid Fields...

To analyze the longitudinal change in Bruch's membrane opening minimal rim width (BMO-MRW) and peripapillary retinal nerve fiber layer (pRNFL) thickness using optical coherence tomography (OCT) after implantation of a PRESERFLO® microshunt for surgical glaucoma management adult patients. Retrospective data analysis 59 eyes participants undergoing PRESERFLO between 2019 2022 at tertiary center management. Surgical included primary temporary occlusion shunt to prevent early hypotony. Pre-...

Abstract Background The aim of this study was to compare the outcome implantation a XEN45 Gel Stent with Preserflo MicroShunt in matched‐pair analysis eyes being naïve filtering surgery. Methods In comparative, retrospective study, 50 that had undergone were compared after implantation. Follow‐up at least 6 months, and surgical success measured by criteria A (IOP < 21 mmHg, IOP reduction >20%, no repeat surgery); B 18 C ≤15 ≥40%, surgery). Results After follow‐up period 12 mean...

Background Impairment of neurovascular coupling (NVC) was recently reported in the context subarachnoid hemorrhage and may correlate with disease severity outcome. However, previous techniques to evaluate NVC required invasive procedures. Retinal vessels represent an alternative option for non-invasive assessment NVC. Methods A prototype adapted retinal vessel analyzer used assess diameter mice. Dynamic analysis (DVA) included application monochromatic flicker light impulses predefined...

Abstract Kainic acid ( KA ) is a potent agonist at non‐N‐methyl‐D‐aspartate (non‐ NMDA ionotropic glutamate receptors and commonly used to induce seizures excitotoxicity in animal models of human temporal lobe epilepsy. Among other factors, Ca v 2.3 voltage‐gated calcium channels have been implicated the pathogenesis ‐induced seizures. At physiologically relevant concentrations, endogenous trace metal ions (Cu 2+ , Zn occupy an allosteric binding site on domain I gating module these...

Purpose: To evaluate the rate of and risk factors for rhegmatogenous retinal detachment (RRD) after Descemet membrane endothelial keratoplasty (DMEK) either alone or in combination with cataract surgery. Design: Retrospective analysis prospective database. Methods: Consecutive eyes Fuchs corneal dystrophy that received DMEK surgery a minimum follow-up 1 year between July 2011 January 2021 at Department Ophthalmology University Cologne were analyzed. Exclusion criteria complicated history...

Summary Introduction The relationship between blood metabolites and hemoglobin degradation products (BMHDPs) formed in the cerebrospinal fluid development of vasospasm delayed cerebral ischemia (DCI) after aneurysmal subarachnoid hemorrhage (aSAH) has been focus several previous studies, but their molecular cellular targets remain to be elucidated. Methods Because BMHDP‐induced changes Ca v 2.3 channel function are thought contribute DCI aSAH, we studied modulation by unconjugated bilirubin...

Objective: Metabolic demand increases with neuronal activity and adequate energy supply is ensured by neurovascular coupling (NVC). Impairments of NVC have been reported in the context several diseases may correlate disease severity outcome. Voltage-gated Ca 2+ -channels (VGCCs) are involved regulation vasomotor tone. In present study, we compared arterial venous responses to flicker stimulation v 2.3-competent (Ca 2.3 [+/+] ) -deficient [−/−] mice using retinal vessel analysis. Methods: The...

Purpose: The purpose of this study was to analyze the influence patient positioning on intraocular pressure (IOP) after Descemet membrane endothelial keratoplasty (DMEK) in pseudophakic patients treated for Fuchs corneal dystrophy. Methods: Forty were included prospective, single-blinded, randomized controlled clinical trial. Patients received a YAG iridotomy 1 day before surgery and an 80% anterior chamber tamponade [20% concentration sulfur hexafluoride (SF6)]. Postoperative either supine...

The aim of the present study was to evaluate changes best corrected visual acuity (BCVA), retinal nerve fiber layer thickness (RNFL), total macular volume (TMV), intraocular pressure (IOP) and central (CRT) after intravitreal injection ranibizumab, bevacizumab aflibercept in patients with neovascular age-related degeneration (nAMD) a clinical real world setting.In retrospective design, 120 (80 women 40 men) were analyzed being diagnosed nAMD within 8 years (2010-2018). Every patient received...

Abstract Background/Aims This work aimed to investigate changes in optic nerve head (ONH) morphometry based on Bruch membrane opening children with extensive nocturnal intraocular pressure (IOP) elevations. Methods The course of opening-based was analysed thirty-two patients younger than 18 years evaluable SD-OCT examinations the ONH and posture-dependent IOP elevation above 25 mmHg. Longitudinal neuroretinal rim tissue, as measured by Membrane minimum width (BMO-MRW) peripapillary retinal...

Abstract Background So far, only indirect evidence exists for the pharmacoresistant R-type voltage-gated Ca 2+ channel (VGCC) to be involved in transretinal signaling by triggering GABA-release onto ON-bipolar neurons. This release of inhibitory neurotransmitters was deduced from sensitivity b-wave stimulation Ni , Zn and Cu . To further confirm interpretation these findings, we compared effects application chelation (using kainic acid, KA) on neural retina wildtype v 2.3-deficient mice....

Abstract Purpose Currently, there are no specific data on the circadian course of intraocular pressure (IOP) in children, especially for IOP measurements supine position. The study aimed to characterize diurnal and nocturnal fluctuations sitting positions patients less than 18 years age. Methods Seventy-nine eyes 79 under age with suspicious optic nerve heads or ocular hypertension could be included this study. All showed an inconspicuous retinal fiber layer thickness Bruch’s membrane...

Despite the significance of proteins containing iron‐sulfur cluster (Fe–S proteins), processes Fe–S assembly and maturation are poorly understood. However, several key involved in have been identified, notably IscS, a cystein desulfurase, which provides sulfur for IscU, metallochaperone acting as scaffold assembly. In this work, we studied process biosynthesis Trypanosoma brucei by identifying homologue IscS T. (TbIscS). To address function TbIscS, inhibited its expression means RNA...

Abstract Background: So far, only indirect evidence exists for the pharmacoresistant R-type voltage-gated Ca2+ channel (VGCC) to be involved in transretinal signaling by triggering GABA-release onto ON-bipolar neurons. This release of inhibitory neurotransmitters was deduced from sensitivity b-wave stimulation Ni2+ , Zn2+ and Cu2+ . To further confirm interpretation these findings, we compared effects application chelation on neural retina wildtype Cav2.3-deficient mice. Furthermore, effect...

Abstract Background : So far, only indirect evidence exists for the pharmacoresistant R-type voltage-gated Ca 2+ channel (VGCC) to be involved in transretinal signaling by triggering GABA-release onto ON-bipolar neurons. This release of inhibitory neurotransmitters was deduced from sensitivity b-wave stimulation Ni , Zn and Cu . To further confirm interpretation these findings, we compared effects application chelation (using kainic acid, KA) on neural retina wild type v 2.3-deficient mice....

Abstract Background : So far, only indirect evidence exists for the pharmacoresistant R-type voltage-gated Ca 2+ channel (VGCC) to be involved in transretinal signaling by triggering GABA-release onto ON-bipolar neurons. This release of inhibitory neurotransmitters was deduced from sensitivity b-wave stimulation Ni , Zn and Cu . To further confirm interpretation these findings, we compared effects application chelation (using kainic acid, KA) on neural retina wild type v 2.3-deficient mice....

Abstract Background : So far, only indirect evidence exists for the pharmacoresistant R-type voltage-gated Ca 2+ channel (VGCC) to be involved in transretinal signaling by triggering GABA-release onto ON-bipolar neurons. This release of inhibitory neurotransmitters was deduced from sensitivity b-wave stimulation Ni , Zn and Cu . To further confirm interpretation these findings, we compared effects application chelation (using kainic acid, KA) on neural retina wildtype v 2.3-deficient mice....