A5042083317- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- Bone Metabolism and Diseases
- RNA Interference and Gene Delivery
- Bone health and treatments
- S100 Proteins and Annexins
- Muscle Physiology and Disorders
- RNA and protein synthesis mechanisms
- Influenza Virus Research Studies
- Electrohydrodynamics and Fluid Dynamics
- Protein Structure and Dynamics
- Erythrocyte Function and Pathophysiology
- Cancer, Stress, Anesthesia, and Immune Response
- Nanopore and Nanochannel Transport Studies
- Mosquito-borne diseases and control
- Advanced biosensing and bioanalysis techniques
- Force Microscopy Techniques and Applications
- Trace Elements in Health
- HIV Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Cellular Mechanics and Interactions
- Magnetic and Electromagnetic Effects
- Viral Infections and Vectors
National Institutes of Health
2015-2024
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2015-2024
Institute of Neuroimmunology of the Slovak Academy of Sciences
2024
Helmholtz-Zentrum Dresden-Rossendorf
2024
University Hospital Carl Gustav Carus
2024
Health and Human Development (2HD) Research Network
1995-2023
Eunice Kennedy Shriver Center
2023
Glenville State College
2017
Milbank Memorial Fund
2014
Tel Aviv University
2003
Cellular uptake of a family cationic cell-penetrating peptides (examples include Tat and penetratin) have been ascribed in the literature to mechanism that does not involve endocytosis. In this work we reevaluate mechanisms cellular 48-60 (Arg)(9). We demonstrate here cell fixation, even mild conditions, leads artifactual these peptides. Moreover, show flow cytometry analysis cannot be used validly evaluate unless step trypsin digestion membrane-adsorbed peptide is included protocol....
Delivery of macromolecules mediated by protein transduction domains (PTDs) attracts a lot interest due to its therapeutic and biotechnological potential. A major reevaluation the mechanism PTD-mediated internalization role endocytosis in this has been recently initiated. Here, we demonstrate that entry TAT peptide (one most widely used PTDs) into different primary cells is ATPand temperature-dependent, indicating involvement endocytosis. Specific inhibitors clathrin-dependent partially...
Many enveloped viruses invade cells via endocytosis and use different environmental factors as triggers for virus-endosome fusion that delivers viral genome into cytosol. Intriguingly, dengue virus (DEN), the most prevalent mosquito-borne infects up to 100 million people each year, fuses only in late endosomes, while activation of DEN protein fusogen glycoprotein E is triggered already at pH characteristic early endosomes. Are there any cofactors time virion entry endosomes? Here we show...
The mechanism of bilayer unification in biological fusion is unclear. We reversibly arrested hemagglutinin (HA)-mediated cell-cell right before pore opening. A low-pH conformation HA was required to form this intermediate and ensure beyond it. present evidence indicating that outer monolayers the fusing membranes were merged continuous intermediate, but restricted lipid mixing. Depending on surface density membrane composition, hemifusion either transformed into a or expanded an unrestricted...
While the specificity and timing of membrane fusion in diverse physiological reactions, including virus–cell fusion, is determined by proteins, always involves merger lipid bilayers. We have isolated a lipid-dependent stage cell–cell mediated influenza hemagglutinin triggered cell exposure to mildly acidic pH. This preceded actual pore formation but was subsequent low pH–induced change conformation that required for fusion. A pH achieve this also, downstream it, drive completion. The lower...
For the act of membrane fusion, there are two competing, mutually exclusive molecular models that differ in structure initial pore, pathway for ionic continuity between formerly separated volumes. Because biological “fusion pores” can be as small channels or gap junctions, one model posits a proteinaceous fusion pore. pore conductance varies widely, another proposes lipidic We have found opening and flickering during protein-free phospholipid vesicles with planar bilayers. Fusion formation...