A5015274437- RNA Interference and Gene Delivery
- Electrospun Nanofibers in Biomedical Applications
- Nanoparticle-Based Drug Delivery
- Bone Tissue Engineering Materials
- Salivary Gland Disorders and Functions
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Optical Imaging and Spectroscopy Techniques
- Oral microbiology and periodontitis research
- Photoacoustic and Ultrasonic Imaging
- Advanced Polymer Synthesis and Characterization
- Effects of Radiation Exposure
- Proteoglycans and glycosaminoglycans research
- Nerve injury and regeneration
- Hydrogels: synthesis, properties, applications
- Polymer Surface Interaction Studies
- Bacterial biofilms and quorum sensing
- Tendon Structure and Treatment
- Graphene and Nanomaterials Applications
- Periodontal Regeneration and Treatments
- Bone and Dental Protein Studies
- Glycosylation and Glycoproteins Research
University of Rochester
2016-2025
University of Rochester Medical Center
2016-2025
University of Oregon
2022-2025
Impact Technology Development (United States)
2024
Eugene Research Institute
2023
John Wiley & Sons (United States)
2020-2021
Smith College
2020-2021
Syracuse University
2020-2021
Eastman Chemical Company (United States)
2021
Materials Science & Engineering
2020
Development of effective therapies to control oral biofilms is challenging, as topically introduced agents must avoid rapid clearance from biofilm-tooth interfaces while targeting biofilm microenvironments. Additionally, exopolysaccharides-matrix and acidification microenvironments are associated with cariogenic (caries-producing) virulence. Thus, nanoparticle carriers capable binding hydroxyapatite (HA), saliva-coated HA (sHA), exopolysaccharides enhanced drug release at acidic pH were...
Satisfactory healing following acute tendon injury is marred by fibrosis. Despite the high frequency of injuries and poor outcomes, there are no pharmacological therapies in use to enhance process. Moreover, systemic treatments demonstrate homing, limiting beneficial effects potential therapeutics. To address this unmet need, we leveraged our existing spatial transcriptomics dataset identified an area enriched for expression
Hydrogels were prepared by copolymerizing a degradable macromer, poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) endcapped with methacrylate groups (PEG-LA-DM), nondegradable poly(ethylene glycol) dimethacrylate (PEGDM). Copolymer networks consisted of 100:0, 83:17, 67:33, and 50:50 PEGDM:PEG-LA-DM mass%, essentially creating scaffolds that exhibit 0, 17, 33, 50% degradation over the time course experiment. Osteoblasts photoencapsulated in these copolymer hydrogels cultured for...
Peptides derived from the third B-cell lymphoma 2 (Bcl-2) homology domain (BH3) can heterodimerize with antiapoptotic Bcl-2 family members to block their activity and trigger apoptosis. Use of these peptides presents a viable anticancer approach, but delivery barriers limit broad application intracellular-acting as clinical therapeutics. Here, novel diblock copolymer carrier is described that confers desirable pharmaceutical properties therapeutic through site-specific molecular conjugation....
Small interfering RNA (siRNA)-based therapies have great potential for the treatment of debilitating diseases such as cancer, but an effective delivery strategy siRNA is elusive. Here, pH-responsive complexes were developed in order to sensitize drug-resistant ovarian cancer cells (NCI/ADR-RES) doxorubicin. The electrostatic consisted a cationic micelle used nucleating core, siRNA, and endosomolytic polymer. Cationic micelles formed from diblock copolymers dimethylaminoethyl methacrylate...
Radiation treatment of head and neck cancers causes irreversible damage the salivary glands (SG). Here, we introduce a preclinical mouse model for small-interfering RNA (siRNA)-based gene silencing to provide protection SG from radiation-induced apoptosis. Novel, pH-responsive nanoparticles complexed with siRNAs were introduced into submandibular (SMG) by retroductal injection modulate expression in vivo. To validate this approach, first targeted Nkcc1, an ion transporter that is essential...
Novel polymer nanoparticle formulation improved drug loading, demonstrated zero-order release, and amplified anti-biofilm activity <italic>via</italic> increased bacterial membrane localization.
Corona and core molecular weights of p(DMAEMA)-<italic>b</italic>-p(DMAEMA-<italic>co</italic>-BMA-<italic>co</italic>-PAA) block copolymer micelles can be tuned to enhance drug release in response acidic milieus consistent with oral biofilms.
Amphiphilic diblock copolymers of poly(styrene-alt-maleic anhydride)-b-poly(styrene) (PSMA-b-PS) and anhydride)-b-poly(butyl acrylate) (PSMA-b-PBA) were synthesized via reversible addition–fragmentation chain transfer (RAFT) polymerizations. Polymers well-controlled with respect to molecular weight evolution polydispersity indices (PDI < 1.2). Additionally, RAFT allowed for control compositions (i.e., ratio hydrophilic PSMA blocks hydrophobic PS/PBA blocks) overall weight, which resulted in...
In this study, a non-native chemical species, bromodeoxyuridine (BrdU), was imaged within single HeLa cells using time-of-flight secondary ion mass spectrometry (TOF-SIMS). z-corrected 3D images were reconstructed that accurately portray the distribution of intracellular BrdU as well other structures. The localized to nucleus cells, whereas structures composed CxHyOz– species located in bundles on periphery cells. subcellular features had spatial resolution at or slightly below micrometer...
Despite several decades of progress, bone-specific drug delivery is still a major challenge. Current bone-acting drugs require high-dose systemic administration which decreases therapeutic efficacy and increases off-target tissue effects. Here, bone-targeted nanoparticle (NP) system for β-catenin agonist, 3-amino-6-(4-((4-methylpiperazin-1-yl)sulfonyl)phenyl)-N-(pyridin-3-yl)pyrazine-2-carboxamide, glycogen synthase kinase 3 beta (GSK-3β) inhibitor, was developed to enhance fracture healing....