A5015926296- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Fatty Acid Research and Health
- Advanced Glycation End Products research
- Eicosanoids and Hypertension Pharmacology
- Fibroblast Growth Factor Research
- Inflammatory mediators and NSAID effects
- Protease and Inhibitor Mechanisms
- Liver physiology and pathology
- Peroxisome Proliferator-Activated Receptors
- Retinopathy of Prematurity Studies
- Wound Healing and Treatments
- Lipid metabolism and biosynthesis
- Biochemical effects in animals
- Antiplatelet Therapy and Cardiovascular Diseases
- Proteoglycans and glycosaminoglycans research
- Connective tissue disorders research
- Protein Tyrosine Phosphatases
- Retinoids in leukemia and cellular processes
- Receptor Mechanisms and Signaling
- Retinal Diseases and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Mitochondrial Function and Pathology
Louisiana State University Health Sciences Center New Orleans
2014-2023
Louisiana State University
1996-2013
University of New Orleans
2013
Association for Research in Vision and Ophthalmology
2010
Ochsner Medical Center
2006
Eye Center
2005
Center for Neurosciences
2004
University Medical Center New Orleans
1992-2003
New York Medical College
1994
Royal National Throat Nose and Ear Hospital
1986
One important action of growth factors is their participation in tissue repair; however, the signaling pathways involved are poorly understood. In a model corneal wound healing, we found that two paracrine factors, hepatocyte factor (HGF) and keratinocyte (KGF), induced rapid marked activation prompt nuclear accumulation phospho-p38 (p-p38) -ERK1/2 (p-ERK1/2), but not JNK (p-JNK1/2), epithelial cells. Interruption p38 ERK1/2 by pretreatment with inhibitors SB203580 PD98059 subsequent...
Abstract— We have found the levels of free fatty acids (FFA) in rat brain to be much lower than had been previously supposed. An unusually rapid and linear production FFA occurred brains adult rats during period from 30 s 4 min after decapitation. Under same conditions newborn or liver spleen was slower. The major sources produced under these were lipids other triglycerides. FFA, like norepinephrine, higher concentration hypothalamic region stem remainder brain. proposed a hypothesis...
Purpose: Dry eye (DE) is a common ocular surface disease, particularly among women and the elderly, with chronic symptoms of irritation and, in severe cases, blurred vision. Several studies have shown that there an inflammatory component DE, although pathogenesis not thoroughly understood. Resolvin E1 (RvE1; RX-10001) endogenous mediator derived from omega-3 polyunsaturated fatty acid eicosapentaenoic involved inflammation resolution tissue protection. Here we investigated role RvE1 DE mouse...
Purpose: To investigate the entire nerve architecture and content of two main sensory neuropeptides in mouse cornea to determine if it is a good model with similarities human corneal innervation. Methods: Mice aged 1 24 weeks were used. The corneas stained neuronal-class βIII-tubulin, calcitonin gene–related peptide (CGRP), substance P (SP) antibodies; whole-mount images acquired build an view test origin CGRP SP, trigeminal ganglia (TG) processed for immunofluorescence. Relative fiber...
This study was conducted to define whether pigment epithelial-derived growth factor (PEDF), together with docosahexaenoic acid (DHA), enhances the synthesis of neuroprotectin D1 (NPD1) and regeneration corneal nerves damaged after surgery.Corneal stromal dissection performed in left eyes adult New Zealand rabbits treated DHA+PEDF, PEDF, or DHA for 6 weeks. In vivo confocal images corneas were obtained at 2, 4, 8 weeks, nerve areas quantified. At weeks treatment, stained tubulin betaIII...
Diabetic keratopathy decreases corneal sensation and tear secretion delays wound healing after injury. In the current study, we tested effect of treatment with pigment epithelium-derived factor (PEDF) in combination docosahexaenoic acid (DHA) on nerve regeneration a mouse model diabetes or without The study was performed streptozotocin-induced diabetic mice (C57BL/6). Ten weeks streptozotocin injection, showed significant sensitivity, production, epithelial subbasal density when compared...
To assess function of regenerated corneal nerves in correlation with epithelial wound healing after experimental nerve damage rabbits treated pigment epithelial-derived factor (PEDF) plus docosahexaenoic acid (DHA).An 8-mm stromal dissection was performed the right eyes adult New Zealand rabbits. Treatment PEDF+DHA for 6 weeks. Corneal sensation measured weekly by Cochet-Bonnet esthesiometer. After 8 weeks, immunofluorescence βIII-tubulin, calcitonin gene-related peptide, and substance P...
To investigate if topical treatment of neuroprotectin D1 (NPD1) increases regeneration functional nerves after lamellar keratectomy.An 8-mm stromal dissection was performed in the left eye each rabbit. The rabbits were treated with NPD1, pigment epithelial-derived factor (PEDF) combination docosahexaenoic acid (DHA) or vehicle for 6 weeks, and corneas obtained at 8 weeks. After fixation, corneal wholemounts stained mouse monoclonal anti-βIII-tubulin antibody double chicken anti-calcitonin...
purpose. To investigate the effect of nerve growth factor (NGF) in combination with docosahexaenoic acid (DHA) on corneal regeneration a rabbit model after PRK and correlate findings functional tear test results. methods. Unilateral was performed 21 New Zealand albino rabbits. Three groups, each consisting six rabbits, were randomized to receive twice-weekly treatments DHA, NGF, or NGF plus DHA delivered by collagen shield. A fourth group, control, received treatment albumin. Rabbits...
Platelet-activating factor (PAF), a potent lipid mediator involved in inflammatory and immune responses, accumulates rapidly response to injury variety of tissues, including the corneal epithelium. However, precise role this compound cascade events following insult has not been defined. Here we examined effect PAF on gene expression epithelial cells rabbit corneas organ culture. We found that incubation with 100 nM methylcarbamoyl PAF, nonhydrolyzable analog produced rapid transient 2.8-...
The cyclooxygenase and lipoxygenase pathways that produce prostaglandins (PGs) hydroxyeico-satetraenoic acids (HETEs) were studied in inflamed rabbit cornea. A cryogenic lesion was induced five days later the epithelium, stroma endothelium isolated incubated with [1-14c]arachidonic acid. After lesioning, arachidonic acid metabolites, thromboxane B2, PGF2a 6-keto-PGFla exhibited greatest increase stroma. Lipoxygenase products formed three layers also, although 12-HETE predominated....
To investigate the action of epidermal growth factor (EGF) on corneal keratocyte differentiation and its effects in conjunction with transforming (TGF)-beta1.Rabbit keratocytes (RCKs) were treated EGF, TGF-beta1, or EGF plus TGF-beta1 presence absence inhibitors EGF-receptor (EGF-R), neutralizing concentrations antibody signaling kinases for 2 days to 1 week. RCK myofibroblasts was identified anti-aldehyde dehydrogenase (ALDH)-1 alpha-smooth muscle actin (alpha-SMA) antibodies. Cell...
Purpose.: To compare a 44-mer pigment epithelial–derived factor (PEDF) peptide with neurotrophic activity, and 34-mer PEDF antiangiogenic properties in association docosahexaenoic acid (DHA) corneal nerve regeneration after experimental surgery. Methods.: A stromal dissection was performed rabbits. Treatment groups received topical 44-mer, 34-mer, or full plus DHA. Corneal sensitivity Schirmer's test were weekly. Rabbits euthanized at 2 4 days 8 weeks. Two- 4-day samples stained for...
The high-density corneal innervation plays a pivotal role in sustaining the integrity of ocular surface. We have previously demonstrated that pigment epithelium-derived factor (PEDF) plus docosahexaenoic acid (DHA) promotes nerve regeneration; here, we report mechanism involved and discovery stereospecific Resolvin D6-isomer (RvD6si) drives process. RvD6si wound healing functional recovery by restoring after injury. applied to eye surface elicits specific transcriptome signature trigeminal...
Cell survival is critical during corneal epithelial regeneration after injury, and growth factors could be fundamental in cytoprotection. The goal of this study was to investigate the involvement paracrine hepatocyte factor (HGF) prevention cell apoptosis identify signal transducers process.Apoptosis human rabbit (HCE RCE) cells induced with a nutrient-deprived exhausted medium (ExM) or by treatment staurosporine (20-100 ng/mL) calcium ionophore A23187 (0.5 microM). Apoptotic were identified...