A5075150921- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Studies on Chitinases and Chitosanases
- Legume Nitrogen Fixing Symbiosis
- Research in Cotton Cultivation
- Insect Resistance and Genetics
- Cellular Mechanics and Interactions
- Gas Sensing Nanomaterials and Sensors
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Resistance and Genetics
- Signaling Pathways in Disease
- Plant Stress Responses and Tolerance
- Microbial Natural Products and Biosynthesis
- Redox biology and oxidative stress
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Plant pathogens and resistance mechanisms
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Cancer therapeutics and mechanisms
- Transition Metal Oxide Nanomaterials
- Polysaccharides and Plant Cell Walls
- Nanoparticles: synthesis and applications
- Cancer Genomics and Diagnostics
Fujian Agriculture and Forestry University
2019-2025
Chinese Academy of Sciences
2011-2021
State Key Laboratory of Plant Genomics
2011-2021
Institute of Microbiology
2016-2021
University of Chinese Academy of Sciences
2016-2021
Tiangong University
2017
Examining the proteins that plants secrete into apoplast in response to pathogen attack provides crucial information for understanding molecular mechanisms underlying plant innate immunity. In this study, we analyzed changes root secretome of Verticillium wilt-resistant island cotton cv Hai 7124 (Gossypium barbadense) upon infection with dahliae. Two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry analysis...
The apoplast serves as the first battlefield between plant hosts and invading microbes; therefore, work on plant-pathogen interactions has increasingly focused apoplastic immunity. In this study, we identified three proteins in of cotton (Gossypium sp) root cells during interaction with fungal pathogen Verticillium dahliae. Among these proteins, host secrete chitinase 28 (Chi28) Cys-rich repeat protein 1 (CRR1), while releases protease VdSSEP1. Biochemical analysis demonstrated that VdSSEP1...
In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, 1.11.1.11), and catalase (CAT, l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. present study, we isolated three homologous genes (GhSOD1, GhAPX1, GhCAT1) Gossypium hirsutum. Overexpressing cassettes containing chimeric GhSOD1, or GhCAT1 were introduced into cotton plants by Agrobacterium transformation, overexpressed products these transported...
Summary Rice blast disease caused by Magnaporthe oryzae poses a severe threat to rice production. To counteract M. , plants synthesize jasmonate (JA) and lignin, two primary defense‐related metabolites, initiate defense programs. However, the mechanism through which modulates JA‐ lignin‐mediated plant immunity remains unclear. In this study, novel effector, MoBys1, was identified as being involved in pathogenesis. Knockout of MoBys1 significantly reduced its infection ability. Conversely,...
ABSTRACT Magnaporthe oryzae ( M. ) is a devastating hemibiotrophic pathogen. Its biotrophic invasive hyphae (IH) are enclosed in the extrainvasive hyphal membrane produced by plant cells, thus generating front line of battlefield between pathogen and host plants. In plants, defense‐related complexes such as proteins, callose‐rich materials vesicles, directionally secreted to this interface confer defense responses, but underlying molecular mechanism poorly understood. study, we found that...
Several studies have revealed that actin depolymerizing factors (ADFs) participate in plant defence responses; however, the functional mechanisms appear intricate and need further exploration. In this study, we identified an ADF6 gene upland cotton (designated as GhADF6) is evidently involved cotton's response to fungal pathogen Verticillium dahliae. GhADF6 binds filaments possesses severing activities vitro vivo. When root (the site of fungus invasion) was inoculated with pathogen,...
Cytoskeletal microtubules (MTs) play crucial roles in many aspects of life processes eukaryotic organisms. They dynamically assemble physiologically important MT arrays under different cell conditions. Currently, assembly underlying the development and pathogenesis model plant pathogenic fungus
Our previous study demonstrated that WLIM1a has dual roles in fiber elongation and secondary cell wall synthesis upland cotton, the protein acts either as an actin-binding or a transcription factor. Because consists of two different LIM domains, it is possible these elements contribute differentially to functions protein. In this study, we dissected domains characterized their biochemical functions. By using red fluorescent (RFP) fusion, co-sedimentation, DNA binding methods, found WLIM1a,...
Abstract Actin assembly at the hyphal tip is key for polar growth and pathogenesis of rice blast fungus Magnaporthe oryzae . The mechanism its precise assemblies biological functions not understood. Here, we characterized role M. Twinfilin (MoTwf) in M infection through organizing actin cables that connect to Spitzenkörper (Spk) tip. MoTwf could bind bundle filaments. It formed a complex with Myosin2 (MoMyo2) Woronin body protein Hexagonal peroxisome 1 (MoHex1). Enrichment MoMyo2 MoHex1...
LIM proteins are widely spread in various types of plant cells and play diversely crucial cellular roles through actin cytoskeleton assembly gene expression regulation. Till now, it has not been clear whether function pathogen defense. In this study, we characterized a protein, GhWLIM1C, upland cotton (Gossypium hirsutum). We found that GhWLIM1C could bind bundle the cytoskeleton, contains two domains (LIM1 LIM2). Both directly to filaments. Moreover, LIM2 domain additionally bundles...
Abstract Polarized actin cable from Spitzenkörper at the hyphal tip fuels filamentous growth in diverse biphasic fungal pathogens. This multi-component complex, featuring nucleator Bni1 and other factors, initiates polymerization, guiding host infection. How dynamic assembly of are achieved to support fungi that undergo multistage morphogenesis for invasion remains unclear, including Magnaporthe oryzae , which undergoes morphological transition during rice Here, we identified scaffolder...
Thin films composed of high pure VO 2 (A) doped W 6+ ions were successfully prepared by an inorganic sol-gel method.The effects doping on the structure, surface morphology and electrical property investigated.The results show that solution distorted lattice film, made parameter d increase.With concentration increase, grain size film increased, shape became rod or block from sphere.The resistance decreases sharply, near one magnitude with increase 0 to 0.75 at%.And TCR close zero a negative...
Abstract Osimertinib (AZD9291) is the first-line treatment for EGFR-mutated NSCLC; however, majority of patients inevitably develop resistance due to de novo genomic abnormalities, such as C797S mutation, EGFR exon 20 insertion, MET amplification and other unknown mechanisms. Hence, effective therapies overcome acquired are urgently needed. Inhibition BCL-2/BCL-xL has been reported enhance apoptosis in EGFR-TKI resistant cells with low sensitivity inhibition. In this study, we evaluated...