Hepatitis B and C viruses (HBV HCV, respectively) are associated with acute chronic liver diseases hepatocellular carcinoma. To elucidate the molecular status of superinfection these two hepatitis viruses, we cotransfected full-length or truncated version HCV structural genes (core envelope 1) together cloned HBV DNA into a human hepatoma cell line (HuH-7). Expression HBV-specific major transcripts (3.5 2.1 kb), as well antigens (hepatitis surface antigen e core antigens), was reduced about...

Nuclear export of mRNA is tightly linked to transcription, nuclear processing, and subsequent maturation in the cytoplasm. Tip-associated protein (TAP) major receptor, it acts coordinately with various factors involved expression. We screened for that associate TAP identified several candidates, including RNA helicase DDX3. demonstrate DDX3 directly interacts its association as well ribonucleoprotein complexes may occur nucleus. Depletion resulted accumulation DDX3, suggesting is, at least...

Upon environmental insults, SGs (stress granules) aid cell survival by serving as sites of translational silencing. RNA helicase DDX3 was reported to associate with SGs. However, its role in SG physiology remains undefined. We have demonstrated previously that acts an eIF4E (eukaryotic initiation factor 4E)-inhibitory protein suppress translation. In the present study, we indentified marker PABP1 [poly(A)-binding 1] another direct interaction partner DDX3. established various stimuli novel...

Abstract DDX3 is a DEAD box RNA helicase with diverse biological functions. Using colony formation assay, our results revealed that inhibited the ability of various tumor cells, and this inhibition might be due to reduced growth rate caused by DDX3. Additionally, we identified p21waf1/cip1, cyclin-dependent kinase inhibitor, as target gene DDX3, up-regulation p21waf1/cip1 expression accounted for colony-suppressing activity Moreover, exerted its transactivation function on promoter through...

Abstract Alterations in membrane proteins (MPs) and their regulated pathways have been established as cancer hallmarks extensively targeted clinical applications. However, the analysis of MP-interacting downstream across human malignancies remains challenging. Here, we present a systematically integrated method to generate resource protein-regulated networks (CaMPNets), containing 63,746 high-confidence protein–protein interactions (PPIs) for 1962 MPs, using expression profiles from 5922...

The X-linked DEAD-box RNA helicase DDX3 (DDX3X) is a multifunctional protein that has been implicated in gene regulation, cell cycle control, apoptosis, and tumorigenesis. However, the precise physiological function of Ddx3x during development remains unknown. Here, we show loss results an early post-implantation lethality male mice. size epiblast marked by Oct3/4 dramatically reduced embryonic day 6.5 (E6.5) Ddx3x−/Y embryos. Preferential paternal X chromosome inactivation (XCI)...

ABSTRACT The nucleocapsid core protein of hepatitis C virus (HCV) has been shown to trans -act on several viral or cellular promoters. To get insight into the -action mechanism HCV protein, a yeast two-hybrid cloning system was used for identification protein-interacting protein. One such cDNA clone encoding DEAD box family putative RNA helicase obtained. This helicase, designated CAP-Rf, exhibits more than 95% amino acid sequence identity other known helicases including human DBX and DBY,...

ABSTRACT Our previous study indicated that the core protein of hepatitis C virus (HCV) can associate with tumor necrosis factor receptor (TNFR)-related lymphotoxin-β (LT-βR) and this protein-protein interaction plays a modulatory effect on cytolytic activity recombinant form LT-βR ligand (LT-α1β2) but not alpha (TNF-α) in certain cell types. Since both TNF-α/TNFR LT-α1β2/LT-βR are also engaged transcriptional activator NF-κB activation or c-Jun N-terminal kinase (JNK) activation, biological...

We previously demonstrated that the core protein of hepatitis C virus (HCV) can suppress gene expression and replication B (HBV) in a human hepatoma cell line (HuH-7). In this study, we have characterized phosphorylation property HCV examined effect on its suppressive activity HBV. Our results indicated both full-length (22 kDa) processed or degraded forms (14 to 18 were phosphorylated insect cells. As by using glutathione S-transferase fusion system vitro transcription translation system,...

We have demonstrated previously that the core protein of hepatitis C virus (HCV) exhibits suppression activity on gene expression and replication B (HBV). Here we further elucidated mechanism HCV protein. retained inhibitory effect HBV when expressed as part full length polyprotein. Based substitution mutational analysis, our results suggested mutation introduced into bipartite nuclear localization signal resulted in cytoplasmic but did not affect its ability expression. Mutational studies...

Previous studies suggest that the core protein of hepatitis C virus (HCV) has a pleiotropic function in replication cycle virus. To understand role this HCV pathogenesis, we used yeast two-hybrid interaction cloning system to search for cellular proteins physically interacting with protein. One such gene was isolated and characterized as encoding lymphotoxin-beta receptor (LT-betaR). In vitro binding analysis demonstrated binds C-terminal 98 amino acids within intracellular domain LT-betaR...

Hepatocellular carcinoma (HCC) is the fifth common cancer in world and it mainly occurs men. Glycine N-methyltransferase (GNMT) participates one-carbon metabolism affects DNA methylation by regulating ratio of S-adenosylmethionine to S-adenosylhomocystine. Previously, we described that expression GNMT was diminished human HCC. Here, showed 50% (3/6) male 100% (7/7) female Gnmt-/- mice developed HCC, their mean ages HCC development were 17 16.5 months, respectively. In addition, 42.9% (3/7)...

Hepatitis delta virus (HDV) encodes two isoforms of antigens (HDAgs). The small form HDAg is required for HDV RNA replication, while the large inhibits viral replication and virion assembly. In this study, we found that expression B23, a nucleolar phosphoprotein involved in disparate functions including nuclear transport, cellular proliferation, ribosome biogenesis, up-regulated by these HDAgs. Using vivo vitro experimental approaches, have demonstrated both can interact with B23 their...

genes that encode MelC1 and MelC2 proteins.MelC1 has been suggested as a transactivator which can facilitate the incorporation of copper into apotyrosinase (MelC2) (Lee, Y.

The Notch signal pathway plays multifaceted roles to promote or suppress tumorigenesis. Notch1 receptor intracellular domain (N1IC), the activated form of receptor, activates c-myc proto-oncogene. complex N1IC and transcription factor YY1 binds human promoter enhance expression in a CBF1-independent manner. Here we demonstrated that interacted with c-Myc-regulating proteins alpha-enolase binding protein 1 (MBP-1). Both MBP-1 suppressed N1IC-enhanced activity response element front P2 was...

Glycine N-methyltransferase (GNMT) is a tumor suppressor for hepatocellular carcinoma (HCC). High rates of Gnmt knockout mice developed HCC. Epigenetic alteration and dysregulation several pathways including wingless-type MMTV integration site (Wnt), mitogen-activated protein kinase (MAPK) Janus signal transducer activator transcription (JAK-STAT) are associated with HCC development in mice. We hypothesized that GNMT may regulate transduction through interacting other proteins directly. In...

Abstract Studies indicate that the presence of cancer stem cells (CSCs) is responsible for poor prognosis hepatocellular carcinoma (HCC) patients. In this study, functional role DDX3 in regulation hepatic CSCs was investigated. Our results demonstrated reduced expression not only inversely associated with tumor grade, but also predicted HCC Knockdown cell line HepG2 induced stemness gene signature followed by occurrence self-renewal, chemoreisistance, EMT, migration as well CSC expansion and...

Transcription factor Ying Yang 1 (YY1) indirectly regulates the C promoter-binding (CBF1)-dependent Notch1 signaling via direct interaction with receptor intracellular domain (N1IC) on CBF1-response elements. To evaluate possibility that N1IC might modulate gene expression of YY1 target genes through associating YY1-response elements, we herein investigated effect genes. We found bound to double-stranded oligonucleotides element activate luciferase activity reporter elements a...

Abstract The pleiotropic roles of DEAD-box helicase 3, X-linked (DDX3X), including its functions in transcriptional and translational regulation, chromosome segregation, DNA damage, cell growth control, have highlighted the association between DDX3X tumorigenesis. However, mRNA transcripts protein levels patient specimens shown controversial correlations with hepatocellular carcinoma (HCC) prevalence. In this study, generation hepatocyte-specific Ddx3x-knockout mice revealed that loss Ddx3x...

Environmental stresses threatening cell homeostasis trigger various cellular responses ranging from the activation of survival pathways to eliciting programmed death. Cellular stress response highly depends on nature and level insult as well type. Notably, interplay among all these will ultimately determine fate stressed cell. Human DExD/H RNA helicases are ubiquitous molecular motors rearranging secondary structure in an ATP-dependent fashion. These conserved enzymes participate nearly...

The juxta-anastomotic stenosis of an arteriovenous fistula (AVF) is a significant clinical problem in hemodialysis patients with no effective treatment. Previous studies AV anastomotic angles on hemodynamics and vascular wall injury were based computational fluid dynamics (CFD) simulations using standardized AVF geometry, not the real-world patient images. present study first CFD to use angiographic images patient-specific outcome information, i.e., exact location stenotic lesion. We...

The tyrosinase of Streptomyces antibioticus is encoded by the second open reading frame, melC2 melanin operon (melC). upstream frame melC1 specifies a 146-amino acid protein with typical NH2-terminal signal-peptide characteristic secretory protein. MelC1 involved in transfer copper ion to apotyrosinase MelC2 via binary complex formation (Lee, Y.-H. W., Chen, B.-F., Wu, S.-Y., Leu, W.-M., Lin, J.-J., C. and Lo, S. J. (1988) Gene (Amst.) 65, 71-81; L.-Y., Wang, K.-T., Lee, Y.-H.W. (1992) Biol....