Monacolin K is a secondary metabolite synthesized by polyketide synthases (PKS) from Monascus, and it has the same structure as lovastatin, which mainly produced Aspergillus terreus. In present study, bacterial artificial chromosome (BAC) clone, mps01, was screened BAC library constructed Monascus pilosus BCRC38072 genomic DNA. The putative monacolin biosynthetic gene cluster found within 42 kb region in mps01 clone. deduced amino acid sequences encoded nine genes designated mokA−mokI, share...

Monacolin K is a secondary metabolite synthesized by polyketide synthases (PKS) from Monascus. The monacolin biosynthetic gene cluster, mokA-mokI, has been characterized in Monascus pilosus. mokH encoding Zn(II)2Cys6 binuclear DNA binding protein assumed to be an activator for production. In this study, the was cloned and driven glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter overexpression M. transformants containing extra copy of were obtained verified PCR Southern hybridization....

In the present study, IL-1β cDNA was identified and analyzed from largemouth bass (Micropterus salmoides). Full length mRNA obtained using Rapid Amplification of Ends (RACE), which contains 78 bp 3′-UTR, a 455 5′-UTR, an open reading frame (ORF) 702 coding for 233 amino acid residues. The molecular weight theoretical isoelectric point protein predicted to be 26.7 kDa 6.08 respectively. A phylogenetic analysis showed close relation IL-1βs striped trumpeter (Latris lineata), Chinese perch...

Citrinin, a hepato-nephrotoxic compound to humans, can be produced by the food fermentation microorganisms Monascus spp. In this study, we investigated distribution of mycotoxin citrinin biosynthesis genes in 18 strains. The results show that acyl-transferase and keto-synthase domains pksCT gene encoding polyketide synthase were found purpureus, kaoliang, sanguineus. Furthermore, ctnA gene, major activator for biosynthesis, was M. purpureus but absent orf3 oxygenase, located between ctnA,...

Abstract Background The soil quality and health of the tea plantations are dependent on agriculture management practices, long-term chemical fertilizer use is implicated in decline. Hence, several sustainable practices used to improve maintain quality. Here, this study, changes properties, enzymatic activity, dysbiosis bacterial community composition were compared using three agricultural namely conventional (CA), (SA), transformational (TA) plantation during 2016 2017 period. Soil samples...

The tyrosinase of Streptomyces antibioticus is encoded by the second open reading frame, melC2 melanin operon (melC). upstream frame melC1 specifies a 146-amino acid protein with typical NH2-terminal signal-peptide characteristic secretory protein. MelC1 involved in transfer copper ion to apotyrosinase MelC2 via binary complex formation (Lee, Y.-H. W., Chen, B.-F., Wu, S.-Y., Leu, W.-M., Lin, J.-J., C. and Lo, S. J. (1988) Gene (Amst.) 65, 71-81; L.-Y., Wang, K.-T., Lee, Y.-H.W. (1992) Biol....

In this study, the gene encoding 40 kDa GAPDH of L. garvieae was determined and overexpressed by using Escherichia coli expression system. Analysis results indicated that sequences nucleotide its amino acid are highly homologous (80.4-100%) to several products from other Streptococcus-related bacteria. According Western blotting results, rabbit antiserum tilapia infection serum reacted strongly recombinant protein. another experiment, were immunized intraperitoneally with formalin-killed...

The agarase from Thalassomonas agarivorans BCRC 17492 was cloned and overexpressed in Escherichia coli. characterization of the novel performed.The genomic library T. constructed for screening gene. β-agarase, namely AgaB1, successfully identified shared only 57% identity to reported Alteromonas sp. To characterize AgaB1 protein, recombinant can be obtained by heterologous expression E. activity achieved at 30·25 U per mg 35°C. According analysis optimal conditions, highest attained 40°C, pH...

Abstract Background Medical plants confer various benefits to human health and their bioconversion through microbial fermentation can increase efficacy, reduce toxicity, conserve resources produce new chemical components. In this study, the cholesterol-lowering monacolin K genes content produced by Monascus species were identified. The high-yield strain further fermented with medicinal plants. antioxidant anti-inflammatory activities, red pigment content, total phenolic metabolites in...

The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. transformation efficiency of higher the ATA-treatment than either non-restriction-enzyme-mediated integration (REMI) or REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for genetic transformation.

Abstract Background The soil quality and health of the tea plantations are dependent on agriculture management practices, long-term chemical fertilizer use is implicated in decline. Hence, several sustainable practices used to improve maintain quality. Here, this study, changes properties, enzymatic activity, dysbiosis bacterial community composition were compared using three agricultural namely conventional (CA), (SA) transformational (TA) plantation during 2016 2017 period. Soil samples at...

Thermostable enzymes have the potential as biocatalyst for industrial applications. To compare relationship of enzymatic thermostability, moderately thermophilic and mesophilic bacteria were utilized to explore properties esterases. By using shotgun libraries Thalassomonas agarivorans, Aeromonas sp., Ralstonia esterases-encoding Lip20, Lip4 LipRT α/β-hydrolase fold cloned, sequenced, characterized. According recombinant proteins overexpressed by Escherichia coli, these results indicated that...

Journal Article Nucleotide sequence of the structural genes for mitochondrial asp, lys, ser-tRNAs from chicken Get access Yan-Hwa Lee, Lee * *To whom correspondence should be addressed Search other works by this author on: Oxford Academic PubMed Google Scholar Li-Ling Liaw, Liaw Tsai Tzung Yung, Yung Szecheng J. Lo 1Graduate Program Microbiology and Immunology, National Yang-Ming Medical CollegeTaipei, Taiwan 11221, China Nucleic Acids Research, Volume 17, Issue 22, 25 November 1989, Page...

Abstract Monascus species have been used in Chinese fermented foods such as Anka pork, and rice wine because of its bioactive substances including pigment, monacolin K. In this study, the effect light temperature on red total polyphenols, DPPH radical scavenging, reducing ability K ruber BCRC31535 solid culture was conducted. No obvious difference observed by scavenging assay whatever cultural condition performed. However, results revealed that were highest darkness at 30°C. Blue remarkably...