A5000615612- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Agriculture, Soil, Plant Science
- GABA and Rice Research
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Genetic factors in colorectal cancer
- Computational Drug Discovery Methods
- Pulmonary Hypertension Research and Treatments
- Kruppel-like factors research
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
- Soil Carbon and Nitrogen Dynamics
- Ferroptosis and cancer prognosis
- Lung Cancer Treatments and Mutations
- Circular RNAs in diseases
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Nuclear Receptors and Signaling
- Bioactive natural compounds
- Natural product bioactivities and synthesis
Chinese University of Hong Kong, Shenzhen
2018-2025
Chinese University of Hong Kong
2021-2024
National Chung Hsing University
2021
Zhongshan Hospital of Xiamen University
2021
National Taipei University
2021
China Medical University
2016-2021
Xiamen Medical College
2021
Yenepoya University
2021
Food Industry Research and Development Institute
2021
Shenzhen Maternity and Child Healthcare Hospital
2021
MicroRNAs (miRNAs) are small non-coding RNAs (typically consisting of 18-25 nucleotides) that negatively control expression target genes at the post-transcriptional level. Owing to biological significance miRNAs, miRTarBase was developed provide comprehensive information on experimentally validated miRNA-target interactions (MTIs). To date, database has accumulated >13,404 MTIs from 11,021 articles manual curations. In this update, a text-mining system incorporated enhance recognition...
MicroRNAs (miRNAs) are small non-coding RNA molecules capable of negatively regulating gene expression to control many cellular mechanisms. The miRTarBase database (http://mirtarbase.mbc.nctu.edu.tw/) provides the most current and comprehensive information experimentally validated miRNA-target interactions. was launched in 2010 with data sources for >100 published studies identification miRNA targets, molecular networks targets systems biology, release (2013, version 4) includes significant...
Abstract MicroRNAs (miRNAs) are noncoding RNAs with 18–26 nucleotides; they pair target mRNAs to regulate gene expression and produce significant changes in various physiological pathological processes. In recent years, the interaction between miRNAs their genes has become one of mainstream directions for drug development. As a large-scale biological database that mainly provides miRNA–target interactions (MTIs) verified by experiments, miRTarBase undergone five revisions enhancements. The...
Abstract Background Functional RNA molecules participate in numerous biological processes, ranging from gene regulation to protein synthesis. Analysis of functional motifs and elements sequences can obtain useful information for deciphering regulatory mechanisms. Our previous work, RegRNA, is widely used the identification motifs, this work extends it by incorporating more comprehensive updated data sources analytical approaches into a new platform. Methods results An integrated web-based...
We present MethHC (http://MethHC.mbc.nctu.edu.tw), a database comprising systematic integration of large collection DNA methylation data and mRNA/microRNA expression profiles in human cancer. is an important epigenetic regulator gene transcription, genes with high levels their promoter regions are transcriptionally silent. Increasing numbers being published different public repositories. These can help researchers to identify patterns that for carcinogenesis. integrates such as methylation,...
Endothelial dysfunction plays an integral role in pulmonary hypertension (PH). AMPK (AMP-activated protein kinase) and ACE2 (angiotensin-converting enzyme 2) are crucial endothelial homeostasis. The mechanism by which regulates the endothelium its protective PH remain elusive.We investigated of phosphorylation Ser680 stability deciphered functional consequences this post-translational modification homeostasis PH.Bioinformatics prediction, kinase assay, antibody against phospho-ACE2 (p-ACE2...
Atherosclerosis is a multifaceted inflammatory disease involving cells in the vascular wall (eg, endothelial [ECs]), as well circulating and resident immunogenic monocytes/macrophages). Acting ligand for liver X receptor (LXR), but an inhibitor of SREBP2 (sterol regulatory element-binding protein 2), 25-hydroxycholesterol, its catalyzing enzyme cholesterol-25-hydroxylase (Ch25h) are important regulating cellular status cholesterol biosynthesis both ECs monocytes/macrophages.Bioinformatic...
Abstract In recent years, drug repositioning has emerged as a promising alternative to the time-consuming, expensive and risky process of developing new drugs for diseases. However, current database faces several issues, including insufficient data volume, restricted types, algorithm inaccuracies resulting from neglect multidimensional or heterogeneous data, lack systematic organization literature associated with repositioning, limited analytical capabilities user-unfriendly webpage...
Reciprocal interactions between B and follicular T helper (Tfh) cells orchestrate the germinal center (GC) reaction, a hallmark of humoral immunity. Abnormal GC responses could lead to production pathogenic autoantibodies development autoimmunity. Here we show that miR-146a controls by targeting multiple CD40 signaling pathway components in cells; contrast, loss does not alter responses. However, specific deletion both its paralog, miR-146b, increases Tfh cell numbers enhanced reactions....
Abstract Circular RNAs (circRNAs), which are single-stranded RNA molecules that have individually formed into a covalently closed continuous loop, act as sponges of microRNAs to regulate transcription and translation. CircRNAs important in the field cancer diagnosis, growing evidence suggests they closely related pathological features. Therefore, high potential for clinical use novel biomarkers. In this article, we present our updates CircNet (version 2.0), circRNAs from circAtlas...
Objective— The topographical distribution of atherosclerosis in vasculature underscores the importance shear stress regulating endothelium. With a systems approach integrating sequencing data, current study aims to explore link between stress-regulated master transcription factor and its regulation endothelial cell (EC) function via epigenetic modifications. Approach Results— H3K27ac (acetylation histone 3 lysine 27)-ChIP-seq (chromatin immunoprecipitation followed by high throughput...
Oncogene-driven stress-related DNA damage has been observed in lesions of colon cancer. Furthermore, sensors and nucleases are stimulated during active replication. However, their changes influences with respect to cancer remain largely unknown.The gene expression levels cGAS, IFI16, STING, TBK1, IFNB1, TREX1, SAMHD1, RNASEH2A, RNASEH2B, RNASEH2C were examined the paired colorectal adjacent normal part tissues 53 patients. Their associations clinical stages then analyzed.All cytosolic...
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with limited treatment options. Despite endothelial cells (ECs) comprising 30% of the cellular composition, role EC dysfunction in (PF) remains unclear. We hypothesize that sterol regulatory element-binding protein 2 (SREBP2) plays critical pathogenesis PF via phenotypic modifications. Transcriptome data demonstrate SREBP2 overexpression ECs led to induction TGF, Wnt, and cytoskeleton remodeling gene ontology pathways...
Dysregulated BMP (bone morphogenetic protein) or TGF-β (transforming growth factor beta) signaling pathways are imperative in idiopathic and familial pulmonary arterial hypertension (PAH) as well experimental (PH) rodent models. MED1 (mediator complex subunit 1) is a key transcriptional co-activator KLF4 (Krüppel-like 4) master transcription endothelium. However, epigenetic regulations of the BMP/TGF-β axes endothelium their dysregulations leading to PAH remain elusive. We investigate...
Background: XueBiJing injection (XBJ) is renowned for its multi-target pharmacological effects, including immunomodulatory, antithrombotic, and antioxidant activities, offering potential therapeutic benefits patients with severe infections such as sepsis Coronavirus disease 2019 (COVID-19). Despite clinical effectiveness, the molecular targets mechanisms of XBJ remain unclear, warranting further investigation. Purpose: This study aimed to identify key bioactive compounds in elucidate their...
Triple-negative breast cancer (TNBC) poses a major clinical challenge due to its aggressive progression and limited treatment options, making early diagnosis prognosis critical. MicroRNAs (miRNAs) are crucial post-transcriptional regulators that influence gene expression. In this study, we unveil novel miRNA-mRNA interactions introduce prognostic model based on miRNA-target interaction (MTI), integrating regulatory correlation inference the machine learning method effectively predict...
As an agri-food by-product, the rice bran of pigmented rice, encompassing varieties such as red, black, and purple has garnered increasing attention due to its richness in terms bioactive compounds. Being mainly composed pericarp, aleuron, seed coat, germ, brown outer layer kernel offers potential health benefits applications skincare. Human skin serves primary barrier against external threats, including pathogens, pollutants, ultraviolet (UV) radiation. Notably, UV radiation accelerates...
Bloodstream infections (BSIs) pose a significant diagnostic challenge, largely due to the limitations of traditional methods such as blood cultures. These often yield low positive rates, have lengthy processing times that delay treatment, and are limited in detecting only narrow range pathogens. Such delays inaccuracies can critically impede timely clinical interventions, potentially compromising patient outcomes. Next-generation sequencing (NGS) is powerful tool for rapid, precise pathogen...
The CRISPR/Cas system is an important aspect in bacterial immunology. anti-phage activity of the CRISPR has been established using synthetic spacers, but vivo studies endogenous spacers are relatively scarce. Here, we showed that bacteriophage P1 titre Escherichia coli decreased glucose-containing medium compared with absence glucose. This glucose effect E. against phage infection disappeared cse3 deletion mutants. on susceptibility to was associated cAMP receptor protein (CRP)-mediated...
This study shows that the expression of yjcC, an in vivo (IVE) gene, and stress response regulatory genes soxR, soxS, rpoS are paraquat inducible Klebsiella pneumoniae CG43. The deletion or soxRS decreased yjcC expression, implying RpoS- SoxRS-dependent control. After H2O2 treatment, reduced bacterial survival. These effects could be complemented by introducing ΔyjcC mutant with YjcC-expression plasmid pJR1. recombinant protein containing only YjcC-EAL domain exhibited phosphodiesterase...
DNA methylation is an important epigenetic regulator in gene expression and has several roles cancer disease progression. MethHC version 2.0 (MethHC 2.0) integrated web-based resource focusing on the aberrant methylomes of human diseases, specifically cancer. This paper presents updated implementation by incorporating additional transcriptomes from public repositories, including 33 cancers, over 50 118 microarray RNA sequencing data TCGA GEO, accumulating up to 3586 manually curated >7000...
Drug–target interactions (DTIs) are considered a crucial component of drug design and discovery. To date, many computational methods were developed for drug–target interactions, but they insufficiently informative accurately predicting DTIs due to the lack experimentally verified negative datasets, inaccurate molecular feature representation, ineffective DTI classifiers. Therefore, we address limitations randomly selecting data from unknown pairs by establishing two validated datasets...
CPEB3 is a sequence-specific RNA-binding protein and represses translation of its target mRNAs in neurons. Here, we have identified novel function as to interact with Stat5b inhibit transcription activity the nucleus without disrupting dimerization, DNA binding nuclear localization Stat5b. Moreover, nucleocytoplasm-shuttling predominant residence cytoplasm; whereas activation NMDA receptors accumulates nucleus. Using knockdown approach, found receptor tyrosine kinase , EGFR, gene...