A5032249264- MicroRNA in disease regulation
- RNA modifications and cancer
- Gut microbiota and health
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Circular RNAs in diseases
- Reproductive tract infections research
- Protein Hydrolysis and Bioactive Peptides
- Prenatal Screening and Diagnostics
- Machine Learning in Bioinformatics
- Genomic variations and chromosomal abnormalities
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Redox biology and oxidative stress
- Lung Cancer Research Studies
- Chemokine receptors and signaling
- Hair Growth and Disorders
- Skin and Cellular Biology Research
- Congenital heart defects research
- Cytokine Signaling Pathways and Interactions
- Ferroptosis and cancer prognosis
- Genetic Associations and Epidemiology
Mackay Medical College
2014-2024
Mackay Memorial Hospital
2012-2024
Mackay Junior College of Medicine, Nursing and Management
2014-2018
National Yang Ming Chiao Tung University
2012-2014
MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides, which negatively regulate the gene expression at post-transcriptional level. This study describes an update miRTarBase (http://miRTarBase.mbc.nctu.edu.tw/) that provides information about experimentally validated miRNA-target interactions (MTIs). The latest expanded it to identify systematically Argonaute-miRNA-RNA from 138 crosslinking and immunoprecipitation sequencing (CLIP-seq) data sets were generated by 21...
MicroRNAs (miRNAs) are small non-coding RNAs of ∼ 22 nucleotides that involved in negative regulation mRNA at the post-transcriptional level. Previously, we developed miRTarBase which provides information about experimentally validated miRNA-target interactions (MTIs). Here, describe an updated database containing 422 517 curated MTIs from 4076 miRNAs and 23 054 target genes collected over 8500 articles. The number by strong evidence has increased ∼1.4-fold since last update 2016. In this...
Abstract Background Functional RNA molecules participate in numerous biological processes, ranging from gene regulation to protein synthesis. Analysis of functional motifs and elements sequences can obtain useful information for deciphering regulatory mechanisms. Our previous work, RegRNA, is widely used the identification motifs, this work extends it by incorporating more comprehensive updated data sources analytical approaches into a new platform. Methods results An integrated web-based...
Circular RNAs (circRNAs) represent a new type of regulatory noncoding RNA that only recently has been identified and cataloged. Emerging evidence indicates circRNAs exert layer post-transcriptional regulation gene expression. In this study, we utilized transcriptome sequencing datasets to systematically identify the expression (including known newly ones by our pipeline) in 464 RNA-seq samples, then constructed CircNet database (http://circnet.mbc.nctu.edu.tw/) provides following resources:...
We present MethHC (http://MethHC.mbc.nctu.edu.tw), a database comprising systematic integration of large collection DNA methylation data and mRNA/microRNA expression profiles in human cancer. is an important epigenetic regulator gene transcription, genes with high levels their promoter regions are transcriptionally silent. Increasing numbers being published different public repositories. These can help researchers to identify patterns that for carcinogenesis. integrates such as methylation,...
Some previous studies have identified bacteria in semen as being a potential factor male infertility. However, only few types of were taken into consideration while using PCR-based or culturing methods. Here we present an analysis approach next-generation sequencing technology and bioinformatics to investigate the associations between bacterial communities quality. Ninety-six samples collected examined for communities, measuring seven clinical criteria quality (semen volume, sperm...
Eighty-one stool samples from Taiwanese were collected for analysis of the association between gut flora and obesity. The supervised showed that most, abundant genera bacteria in normal (from people with a body mass index (BMI)<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mo>≤</mml:mo></mml:mrow></mml:math>24) Bacteroides (27.7%), Prevotella (19.4%), Escherichia (12%), Phascolarctobacterium (3.9%), Eubacterium (3.5%). most case (with BMI<mml:math...
Oral squamous cell carcinoma (OSCC) is often diagnosed at a late stage and may be malignantly transformed from oral leukoplakia (OL). This study aimed to identify potential plasma microRNAs (miRNAs) for the early detection of cancer. Plasma normal, OL, OSCC patients were evaluated. Small RNA sequencing was used screen differently expressed miRNAs among groups. Next, these validated with individual samples by quantitative real-time polymerase chain reaction (qRT-PCR) assays in training phase...
Gastrointestinal microbiota, particularly gut is associated with human health. The biodiversity of microbiota affected by ethnicities and environmental factors such as dietary habits or medicine intake, three enterotypes the microbiome were announced in 2011. These are not significantly correlated gender, age, body weight but influenced long-term habits. However, to date, only two (predominantly consisting Bacteroides Prevotella) have shown these characteristics previous research; third...
Protein S-sulfenylation is a type of post-translational modification (PTM) involving the covalent binding hydroxyl group to thiol cysteine amino acid. Recent evidence has shown importance in various biological processes, including transcriptional regulation, apoptosis and cytokine signaling. Determining specific sites fundamental understanding structures functions S-sulfenylated proteins. However, current lack reliable tools often limits researchers use expensive time-consuming laboratory...
Gastric cancer (GC) is the second most frequent cause of cancer-related deaths worldwide. MicroRNAs are single-stranded RNA molecules 21-23 nucleotides that regulate target gene expression through specific base-pairing interactions between miRNA and untranslated regions targeted mRNAs. In this study, we generated a multistep approach for integrated analysis mRNA expression. First, both profiling datasets in gastric from Gene Expression Omnibus (GEO) The Cancer Genome Atlas (TCGA) identified...
Protein carbonylation, an irreversible and non-enzymatic post-translational modification (PTM), is often used as a marker of oxidative stress. When reactive oxygen species (ROS) oxidized the amino acid side chains, carbonyl (CO) groups are produced especially on Lysine (K), Arginine (R), Threonine (T), Proline (P). Nevertheless, due to lack information about carbonylated substrate specificity, we were encouraged develop systematic method for comprehensive investigation protein carbonylation...
Elevated serum haptoglobin (Hp) is identified as a prognostic marker in multiple types of solid tumors, which correlated with poor prognosis. HCC one the major causes cancer deaths worldwide, remains prognosis and clinically urgent for discovering early diagnostic markers. However, except Hp, correlation tumor Hp expression hepatocellular carcinoma (HCC) progression still unclear. In this study, we evaluated tissue to predict survival rate patients. To evaluate value HCC, two cohorts were...
One of the most common and recurrent vaginal infections is bacterial vaginosis (BV). The diagnosis based on changes to "normal" microbiome; however, normal microbiome appears differ according reproductive status ethnicity, even among individuals within these groups. Amsel criteria Nugent score test are widely used for diagnosing BV; tests different criteria, so may indicate distinct in microbial community. Nevertheless, few studies have compared results against metagenomics analysis.Vaginal...
The adipocyte-secreting adipokine, resistin, may play a critical role in the modulation of inflammatory diseases. Migration and infiltration mononuclear cells into sites are events during development osteoarthritis (OA). Monocyte chemoattractant protein-1 (MCP-1), also known as chemokine ligand 2 (CCL2), plays regulation monocyte migration infiltration. In this study, we show how resistin promotes MCP-1 expression OA synovial fibroblasts migration.We used qPCR to detect miRNA expression....
In mammals, microRNAs (miRNAs) play key roles in controlling posttranscriptional regulation through binding to the mRNAs of target genes. Recently, it was discovered that viral miRNAs may be involved human cancers and diseases. It is likely help viruses enter latent phase their life cycle become undetected by host's immune system, while increasing risk for cancer development. Cervical typically related infection papillomavirus (HPV) sexual transmission. To further understand molecular...
The human body plays host to a vast array of bacteria, found in oral cavities, skin, gastrointestinal tract and the vagina. Some bacteria are harmful while others beneficial host. Despite availability many methods identify most them only applicable specific cultivable also tedious. Based on high throughput sequencing technology, this work derives 16S rRNA sequences analyzes probiotics pathogens species.We constructed database that recorded species from literature, along with modified...
The anti-angiogenic fusion protein RBDV-IgG1 Fc (RBDV), which comprises the receptor-binding domain of vascular endothelial growth factor-A (VEGF-A), has shown antitumour effects by reducing angiogenesis in vivo. This study used cationic lipoplex lipo-PEG-PEI-complex (LPPC) to simultaneously encapsulate both RBDV targeting and plasmid (pRBDV) without covalent bonds assess VEGFR gene therapy mice with melanoma vivo.LPPC protected therapeutic transgene from degradation DNase, LPPC/RBDV...
Noncoding, endogenous microRNAs (miRNAs) are fairly well known for regulating gene expression rather than protein coding. Dysregulation of miRNA gene, either upregulated or downregulated, may lead to severe diseases oncogenesis, especially when the disorder involves significant bioreactions pathways. Thus, how genes transcriptionally regulated has been highlighted as target recognition in recent years. In this study, a large-scale investigation novel cis - and trans -elements was undertaken...
Human gut microbiome has an essential role in human health and disease. Although the major dominant microbiota within individuals have been reported, change of caused by external factors, such as antibiotic use bowel cleansing, remains unclear. We conducted this study to investigate overweight male adults after preparation, where none participants had diagnosed with any systemic diseases. A total 20 overweight, Taiwanese were recruited, all omnivorous. The provided fecal samples blood at...