A5036192262- Reproductive System and Pregnancy
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Fungal Biology and Applications
- Ovarian function and disorders
- Soil Carbon and Nitrogen Dynamics
- Gynecological conditions and treatments
- Endometriosis Research and Treatment
- Medicinal Plant Pharmacodynamics Research
- Virus-based gene therapy research
- Advanced Nanomaterials in Catalysis
- Nanocluster Synthesis and Applications
- Autophagy in Disease and Therapy
- Electrospun Nanofibers in Biomedical Applications
- Redox biology and oxidative stress
- Cancer Cells and Metastasis
- Crystallography and molecular interactions
- Gold and Silver Nanoparticles Synthesis and Applications
- Crystallization and Solubility Studies
- Soybean genetics and cultivation
- Phytochemicals and Antioxidant Activities
- melanin and skin pigmentation
- PI3K/AKT/mTOR signaling in cancer
Xiyuan Hospital
2006-2025
Dalian University of Technology
2017-2025
Dalian University
2017-2025
Ningxia University
2007-2025
Luoyang Orthopedic-Traumatological Hospital of Henan Province
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2011-2024
State Administration of Traditional Chinese Medicine of the People's Republic of China
2024
Ministry of Education of the People's Republic of China
2023-2024
Dalian Minzu University
2008-2024
Tsinghua University
2008-2024
Abstract MicroRNAs (miRNAs) are noncoding RNAs with 18–26 nucleotides; they pair target mRNAs to regulate gene expression and produce significant changes in various physiological pathological processes. In recent years, the interaction between miRNAs their genes has become one of mainstream directions for drug development. As a large-scale biological database that mainly provides miRNA–target interactions (MTIs) verified by experiments, miRTarBase undergone five revisions enhancements. The...
Phosphorylation of eukaryotic initiation factor 2 alpha (eIF-2 alpha) is typically associated with stress responses and causes a reduction in protein synthesis. However, we found high phosphorylated eIF-2 alpha[P]) levels nonstressed pancreata mice. Administration glucose stimulated rapid dephosphorylation alpha. Among the four kinases present mammals, PERK most highly expressed pancreas, suggesting that it may be responsible for alpha[P] therein. We describe Perk knockout mutation Pancreata...
Abstract Hepatocellular carcinoma (HCC) is one of the most lethal cancers worldwide. Donafenib a multi‐receptor tyrosine kinase inhibitor approved for treatment patients with advanced HCC, but its clinical effect very limited. Here, through integrated screening small‐molecule library and druggable CRISPR library, that GSK‐J4 synthetically donafenib in liver cancer shown. This synergistic lethality validated multiple HCC models, including xenograft, orthotopically induced patient‐derived...
Phosphorylation of eukaryotic initiation factor-2 (eIF2) by pancreatic eIF2 kinase (PEK), induces a program translational expression in response to accumulation malfolded protein the endoplasmic reticulum (ER). This study addresses mechanisms activating PEK, also designated PERK or EIF2AK3. We describe characterization two regions ER luminal portion transmembrane PEK that carry out distinct functions regulation this kinase. The first region mediates oligomerization between polypeptides, and...
Water-soluble and red-emitting gold nanoclusters (Au NCs) were synthesized with single-stranded DNA as a promising biotemplate dimethylamine borane mild reductant. The fluorescent Au NCs can be formed in weakly acidic aqueous solution that is free from the simultaneous formation of large nanoparticles. cluster feature species has been revealed by fluorescence spectra, absorption transmission electron microscopy. Additionally, sequences could used to tune NCs' emissions. as-prepared display...
Abstract As a member of the phospholipase family, C beta 1 (PLCB1) is involved in phospholipid hydrolysis and frequently upregulated human cancer. However, little known about role PLCB1 cholangiocarcinoma (CCA). In this study, we uncover for CCA progression identify underlying mechanisms. Both tissues cell lines expressed high levels PLCB1. promoted tumor development growth various mouse models, including transposon-based tumorigenesis models. activated PI3K/AKT signaling to induce cells...
Piperlongumine, a natural alkaloid substance extracted from the fruit of long pepper (Piper longum Linn.), is known to inhibit cytosolic thioredoxin reductase (TXNRD1 or TrxR1) and selectively kill cancer cells. However, details mechanism inhibition by piperlongumine against TXNRD1 remain unclear. In this study, based on classical DTNB reducing assay, irreversible recombinant was found showed an apparent kinact value 0.206 × 10−3 µM−1 min−1. Meanwhile, compared with wild-type (-GCUG),...
In response to different cellular stresses, a family of protein kinases regulates translation by phosphorylation the alpha subunit eukaryotic initiation factor-2 (eIF-2alpha). Recently, we identified new member, pancreatic eIF-2alpha kinase (PEK) from rat pancreas. PEK, also referred as RNA-dependent (PKR)-like endoplasmic reticulum (ER) (PERK) is transmembrane implicated in translational control stresses that impair folding ER. this study, and characterized PEK homologues humans, Drosophila...
Pharmaceutical cocrystal has gained increasing interest due to its ability modify various physicochemical properties of hydrophobic drugs, especially solubility and dissolution. The temporarily generated supersaturation during the dissolution cocrystals, usually called "spring parachute" effect, would favor oral absorption poorly soluble drugs. In this study, biopharmaceutics classification system II drug ibuprofen (IBU) was cocrystallized with nicotinamide (NIC) by slow solvent evaporation....
Janus nanoparticles with an anisotropic feature concentrated multiple properties on a single carrier, providing synergistic effects. In this study, dual-functionalized (HA-JMSN/DOX-DMMA) were constructed tumor-targeting ligand (hyaluronic acid, HA) modified the one side and charge reversal group (2,3-dimethylmaleic anhydride, DMMA) other side. The drug release of HA-JMSN/DOX-DMMA was positively correlated acidity environment. cytotoxicity cell uptake superior to isotropous nanoparticles....
Purpose The senescence-accelerated prone mouse 8 (SAMP8) is a widely used model for accelerating aging, especially in central aging. Mounting evidence indicates that the microbiota-gut-brain axis may be involved pathogenesis and progression of aging-related diseases. This study aims to investigate whether Bazi Bushen capsule (BZBS) attenuates deterioration intestinal function aging animal model. Methods In our study, SAMP8 mice were randomly divided into group, BZ-low group (0.5 g/kg/d...
This research aimed to construct an emulsifier by the Maillard reaction at various times using cod fish skin collagen peptide (CSCP) and ι-carrageenan (ι-car) stabilize Antarctic krill oil (AKO) emulsion. emulsion was then investigated for physicochemical stability, oxidative gastrointestinal digestibility. The stability index emulsifying activity of products (MRPs) were increased 36.32 % 66.30 %, respectively, appropriate graft degree (25.58 %) compared with mixture ι-car CSCP. In vitro...
In response to different cellular stresses, a family of protein kinases regulates translation by phosphorylation the α subunit eukaryotic initiation factor-2 (eIF-2α). Recently, we identified new member, pancreatic eIF-2α kinase (PEK) from rat pancreas. PEK, also referred as RNA-dependent (PKR)-like endoplasmic reticulum (ER) (PERK) is transmembrane implicated in translational control stresses that impair folding ER. this study, and characterized PEK homologues humans, Drosophila...
Abstract An important criterion for effective gene therapy is sufficient chromosomal integration activity. The Sleeping Beauty (SB) transposon system a plasmid allowing efficient insertion of transgenes into the host genome. However, such occurs only after delivered to nuclei. Since transposons do not have transducing abilities viral vectors, delivery this first cells and then cell nuclei still challenge. Here, phage display technique using major coat displayed library employed identify...
DNA single-nucleotide polymorphism (SNP) detection has attracted much attention due to mutation related diseases. Various methods for SNP have been proposed and many are already in use. Here, we find that the abasic site (AP site) duplex can be developed as a capping scaffold generation of fluorescent silver nanoclusters (Ag NCs). As proof concept, sequences from fragments near codon 177 cancer supression gene p53 were used model by situ formed Ag NCs. The formation NCs AP site-containing is...
The main objective of this study was to explore whether autophagy could be triggered by cinobufagin, and clarify the role in antitumor effects cinobufagin on U2OS cells underlying mechanisms. were exposed 15, 30, 60 120 mg/l for 0, 12, 24 48 h. An MTT assay used measure cell viability. FITC-Annexin Ⅴ/PI staining flow cytometry analyze apoptotic ratio, while morphological changes assessed PI Hoechst 33258 viable staining. investigated with GFP-LC3b green fluorescence plasmid transfection...