Abstract A bone scaffold material (nano‐HA/ collagen/PLA composite) was developed by biomimetic synthesis. It shows some features of natural both in main composition and hierarchical microstructure. Nano‐hydroxyapatite collagen assembled into mineralized fibril. The three‐dimensional porous materials mimic the microstructure cancellous bone. Cell culture animal model tests showed that composite is bioactive. osteoblasts were separated from neonatal rat calvaria. Osteoblasts adhered, spread,...

A designed hierarchical structure was made by self-assembly of nano-fibrils mineralized collagen resembling extracellular matrix. The fibrils were formed triple helices. Hydroxyapatite (HA) crystals grew on the surface these in such a way that their c-axes oriented along longitudinal axes fibrils. aligned parallel to each other form fibers. For first time, new collagen−hydroxyapatite composite verified conventional and high-resolution transmission electron microscopy.

Acute full-thickness skin wounds (FTSW) caused by extensive burns or high-energy trauma are not adequately addressed current clinical treatments. This study hypothesized that biomimetic nanofiber scaffolds (NFSs) functionalized with rich attachment of bone-marrow-derived mesenchymal stem cells (BM-MSCs) can promote wound healing in acute FTSW. Results a rat model showed both NFS and BM-MSCs contributed to the healing. Wounds group higher density achieved complete closure 8 days earlier than...

The objective of this article was to investigate the in vitro and vivo biodegradation a novel biomimetic bone scaffold composite, nanohydroxyapatite/collagen/poly(L-lactide), that could be used for tissue engineering. For evaluation degradation specimens were immersed into 1% trypsin/phosphate-buffered saline solution at 37 degrees C. In involved implantation samples posterolateral lumbar spine rabbits, retrieved analyzed by Fourier transform-infrared spectroscopy. results demonstrated...

The nucleation sites of calcium phosphate crystals during collagen mineralization were studied by Fourier transform infrared spectrometry and transmission electron microscopy. It was found for the first time that there is another site, i.e., carbonyl (>C=O) on collagen, besides previous reported site carboxyl (–COOH). By comparing IR spectra not only with collagen/calcium but also collagen/Ca 2+ , it observed peak intensities amides I, II, III decreased significantly after mineralization....

Osteoblast cells were separated from the neonatal rat calvaria and co-cultured on a novel mineralized hydroxyapatite/collagen/poly(lactic acid) composite scaffold. By using this static cell culture, three-dimensional osteoblasts/composite bone-like was constructed in vitro. The culture process observed by scanning electron microscopy, fluorescence confocal laser histological analysis. Cells to spread proliferate throughout inner-pores of scaffold material. After 12-day had grown into...

Poly-L-lactic acid (PLLA) and PLLA/collagen (50% PLLA+50% collagen; PLLA/Col) nanofibers were fabricated using electrospinning. Mineralization of these was processed a modified alternating soaking method. The structural properties morphologies mineralized PLLA PLLA/Col investigated X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), contact angle measurements. Human bone-derived osteoblasts cultured on the materials for up to 1 week...

The time required for osseointegration with a metal implant having smooth surface ranges from three to six months. We hypothesized that biomimetic coating surfaces poly(lactic-co-glycolic acid) (PLGA)/collagen fibers and nano-hydroxyapatite (n-HA) on the would enhance adhesion of mesenchymal stem cells. Therefore, this modification dental bone implants might process osseointegration. In study, we coated PLGA or PLGA/collagen (50:50 w/w ratio) fiber Ti disks by modified electrospinning 5 s 2...

In recent years, lots of researches have been launched on nanomaterials for biomedical applications. It has shown that the performances many biomaterials used in prosthodontics significantly enhanced after their scales were reduced by nanotechnology, from micron‐size into nanosize. On other hand, nanocomposites composed and traditional metals, ceramics, resin, or matrix materials widely because properties, such as modulus elasticity, surface hardness, polymerization shrinkage, filler...

A new mineralized collagen matrix combined with or without growth factor was used for the posterolateral spinal fusion in rabbit lumbar spine.The availability of a osteoconductive recombinant osteoinductive factors offers possible alternative to use autogenous bone grafting indications. This study evaluated bone-forming activity biomimetic matrix: nano-hydroxyapatite/collagen/polylactic acid (nHAC/PLA) human morphogenetic protein-2 (rhBMP-2) fusion.Many materials such as titanium alloy,...

Biodegradable nanofibers have become a popular candidate for tissue engineering scaffolds because of their biomimetic structure that physically resembles the extracellular matrix. For certain regeneration applications, prolonged in vitro culture time cellular reorganization and remodeling may be required. Therefore, extensive understanding effects on scaffold degradation is needed. There are only few studies nanofibers, also throughout cell rare. In this study, polyglycolide (PGA),...

By the method of injection molding combined with thermally induced phase separation (TIPS), a novel nerve conduit plurality channels and macro-/microporous architecture was fabricated using poly (lactide-co-glycolide) (PLGA, 75:25; Mn = 1.22 × 105). The diameter conduits number could be regulated by changing parameters mold, porosity as high 95.4%. Meanwhile, hierarchical pore walls controlled through varying solution concentration contents porogen. degradation study in vitro showed that...