A5042950894- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- Erythrocyte Function and Pathophysiology
- Malaria Research and Control
- Mitochondrial Function and Pathology
- Neonatal Health and Biochemistry
- Genetic Associations and Epidemiology
- Prenatal Screening and Diagnostics
- Alzheimer's disease research and treatments
- Mosquito-borne diseases and control
- ATP Synthase and ATPases Research
- Blood groups and transfusion
- Blood disorders and treatments
- Pediatric Hepatobiliary Diseases and Treatments
- Cell Image Analysis Techniques
- Dementia and Cognitive Impairment Research
- RNA modifications and cancer
- Natural Compound Pharmacology Studies
- Blood Coagulation and Thrombosis Mechanisms
- Venomous Animal Envenomation and Studies
- Trypanosoma species research and implications
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Polysaccharides and Plant Cell Walls
- Research on Leishmaniasis Studies
- Natural Antidiabetic Agents Studies
Siriraj Hospital
2006-2023
Mahidol University
2006-2023
Institut Pasteur
2008-2010
Inserm
2008
Office of the Higher Education Commission
2005
Thailand Research Fund
2005
Kobe University
1999
Glucose-6-phosphate dehydrogenase (G6PD) deficiency--the most common known enzymopathy--is associated with neonatal jaundice and hemolytic anemia usually after exposure to certain infections, foods, or medications. Although G6PD-deficient alleles appear confer a protective effect against malaria, the link clinical protection from Plasmodium infection remains unclear. We investigated of G6PD deficiency variant in Southeast Asia--the G6PD-Mahidol(487A) variant--on human survival related vivax...
Background Studies on human genetic factors associated with malaria have hitherto concentrated their role in susceptibility to and protection from disease. In contrast, virtually no attention has been paid the of genetics eliciting production parasite transmission stages, gametocytes, thus enhancing spread Methods Findings We analysed four longitudinal family-based cohort studies Senegal Thailand followed for 2–8 years evaluated relative impact non-genetic gametocyte infections Plasmodium...
Beta-amyloid (A beta) plays a key role in the pathogenesis of Alzheimer's disease (AD) by inducing neurotoxicity and cell death mainly through production reactive oxygen species (ROS). Garcinia mangostana L. (mangosteen) has been recognized as major source natural antioxidants that could decrease ROS. However, its protection A beta-induced cytotoxicity apoptosis neuronal cells remains unclear. We therefore examined such protective effect mangosteen extract (ME) evaluating viability using MTT...
Mitochondria are important organelles responsible for energy production. Mitochondrial dysfunction relates to various pathological diseases. The investigation of mitochondrial heath is critical evaluate the cellular status. Herein, we demonstrated an approach determining status health by observing H2O2 (one type ROS), membrane potential, and morphology (fragmentation length) in live primary fibroblast cells. cells were co-stained with fluorescent dyes (Hoechst 33342 MITO-ID®...
Leber's Hereditary Optic Neuropathy (LHON) is one of the commonest mitochondrial diseases. It causes total blindness, and predominantly affects young males. For disease to develop, it necessary for an individual carry primary mtDNA mutations 11778G>A, 14484T>C or 3460G>A. However these are not sufficient cause disease, they do explain characteristic features LHON such as higher prevalence in males, incomplete penetrance, relatively later age onset. In order explore roles nuclear encoded...
The majority of studies concerning malaria host genetics have focused on individual genes that confer protection against rather than susceptibility to malaria. Establishing the relative impact genetic versus non-genetic factors infection and disease is essential focus effort key determinant factors. This contribution has rarely been evaluated for Plasmodium falciparum almost never vivax. We conducted a longitudinal cohort study in Karen population 3,484 individuals region mesoendemic...
β-Thalassemia/HbE disease has a wide spectrum of clinical phenotypes ranging from asymptomatic to dependent on regular blood transfusions. Ability predict severity is helpful for management and treatment decision making. A thalassemia score been developed Mediterranean β-thalassemia patients. However, different ethnic groups may have allele frequency linkage disequilibrium structures. Here, Thai β0-thalassemia/HbE genome-wild association studies (GWAS) data 487 patients were analyzed by SNP...
We report, herein, an infant who is twin A of a dizygotic twin, with premature birth and both twins having hemoglobin (Hb) E heterozygosity. Twin had Southeast Asian ovalocytosis (SAO) developed neonatal jaundice at the age 2 days needed phototherapy 3 days. The microbilirubin level was rapidly rising up to 535.2 μmol/L (31.3 mg/dl) hematocrit value 38% 4 prior exchange blood transfusion. Exchange transfusion done by 220 ml O, Rh positive packed red cell reconstituted 180 fresh plasma lower...
Protein C (PC) and protein S (PS) play key roles in an anticoagulant pathway order to control the haemostatic system. We identified single nucleotide polymorphisms (SNPs) and/or haplotypes promotor exons of whole PC PS genes 3'-untranslated region gene 55 Thai individuals. The revealed 10 haplotypes. One synonymous SNP at 2196 was found normal population with a minor allele frequency 4.90%. homozygous mutation exon 7, R147W, co-segregated (homozygote) gene, resulting decreased activity...
Plasmodium falciparum malaria episodes may vary considerably in their severity and clinical manifestations.There is good evidence that host genetic factors contribute to this variability.To date, most studies aiming at the identification of these genes have used a case/control study design for severe malaria, exploring specific candidate genes.Here, we performed family-based related phenotypes two independent longitudinal survey cohorts, as first step towards mechanisms involved outcome...
During the last few decades, foetal haemoglobin reactivation has been considered as a promising intervention to treat β-thalassaemia and sickle-cell anaemia.Variable (Hb F, α2γ2) production among individuals is under control of distinct chromosome regions, namely, quantitative trait loci (QTL).One QTL affecting Hb F levels on 8q never explored.The zinc fingers homeodomains 2 (ZHX2) transcription factor located at position remarkably down-regulated in hereditary persistence haemoglobin,...
The advance in genomic technology has allowed us to genotype millions of single nucleotide polymorphisms (SNPs) across the human genome at same time. This high-throughput technology, genome-wide association studies (GWAS), enables search for genes contributing many diseases from age-related macular degeneration, type 2 diabetes, coronary heart disease, cancer common traits such as obesity, height, eye color. To date, there are over 1,779 and 12,126 SNPs reported be associated with more than...
Imbalanced globin chain output contributes to thalassemia pathophysiology. Hence, induction of fetal hemoglobin in β-thalassemia and other β-hemoglobinopathies are continuing interest for therapeutic approaches. Genome-wide association studies have identified three common genetic loci: namely β-globin (HBB), an intergenic region between MYB HBS1L, BCL11A underlying quantitative production. Here, we report that knockdown HBS1L (all known variants) using shRNA early erythroblast obtained from...
We report, herein, an infant who is twin A of a dizygotic twin, with premature birth and both twins having hemoglobin (Hb) E heterozygosity. Twin had Southeast Asian ovalocytosis (SAO) developed neonatal jaundice at the age 2 days needed phototherapy 3 days. The microbilirubin level was rapidly rising up to 535.2 μmol/L (31.3 mg/dl) hematocrit value 38% 4 prior exchange blood transfusion. Exchange transfusion done by 220 ml O, Rh positive packed red cell reconstituted 180 fresh plasma lower...
The diagnosis of Alzheimer disease (AD) primarily comes from clinical assessment although the preclinical pathology occurs several years before cognitive impairment. Recently peripheral marker, platelet amyloid precursor protein ratio, APPr, has been reported as one sensitive markers for early AD with sensitivity (80–100%) and specificity (63–100%). ratio is higher in normal subjects than patients tends to decrease declining functions. Our study aims evaluate usefulness APPr a diagnostic...