A5040422841- Growth Hormone and Insulin-like Growth Factors
- S100 Proteins and Annexins
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
- Neonatal Respiratory Health Research
- Toxic Organic Pollutants Impact
- Birth, Development, and Health
- Cancer, Hypoxia, and Metabolism
- Immune Response and Inflammation
- Infant Nutrition and Health
- Protease and Inhibitor Mechanisms
- Congenital Diaphragmatic Hernia Studies
- Environmental Toxicology and Ecotoxicology
- Antimicrobial Peptides and Activities
- Antimicrobial Resistance in Staphylococcus
- Neonatal and fetal brain pathology
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer-related gene regulation
- Macrophage Migration Inhibitory Factor
- RNA regulation and disease
- Milk Quality and Mastitis in Dairy Cows
- Peroxisome Proliferator-Activated Receptors
- Animal Genetics and Reproduction
- Metabolism and Genetic Disorders
- Effects and risks of endocrine disrupting chemicals
Max Rubner Institut
2008-2024
Giessen School of Theology
2007
University of Massachusetts Chan Medical School
2007
Kerckhoff Klinik
2007
Max Planck Institute for Biophysical Chemistry
2007
University of Münster
1997-1999
University of Auckland
1992-1998
University of Milan
1993
Myeloid-related protein (MRP) 8 and MRP14, two members of the S100 family expressed in myelomonocytic cells, have been ascribed some extracellular functions, e.g. antimicrobial, cytostatic, chemotactic activities. Since proteins lack structural requirements for secretion via classical endoplasmic reticulum/Golgi route, process is unclear. We now demonstrate specific, energy-dependent release MRP8 MRP14 by human monocytes after activation kinase C. This secretory not blocked inhibitors...
Recently, we identified the two myeloid related protein-8 (MRP8) (S100A8) and MRP14 (S100A9) as fatty acid-binding proteins (Klempt, M., Melkonyan, H., Nacken, W., Wiesmann, D., Holtkemper, U., Sorg, C. (1997) <i>FEBS Lett.</i> 408, 81–84). Here present data that S100A8/A9 protein complex represents exclusive arachidonic in human neutrophils. Binding competition studies revealed evidence (i) acid binding was dependent on calcium concentration; (ii) specific for formed by S100A8 S100A9,...
The myeloid-related proteins MRP8 (S100A8) and MRP14 (S100A9), two members of the S100 family calcium-binding proteins, are co-expressed form a cell-surface cytoskeleton-associated heterodimer upon calcium mobilization which is recognized by mAb 27E10. abundantly expressed in cytoplasm granulocytes subpopulation blood monocytes. Previously, we others demonstrated endothelium-associated MRP8/14 inflamed tissues vicinity transmigrating leukocytes, suggesting function this process. Here,...
Abstract As a consequence of ubiquitous use brominated organic chemicals, there is concern for persistent or increasing environmental levels polybrominated dibenzo‐ p ‐dioxins/furans (PBDD/Fs) and mixed polychlorinated di‐benzo‐ (PXDD/Fs). Hence, need to broaden the toxicological knowledge about these compounds, as basis risk assessment. In study presented here, relative potencies (REPs) 18 PBDD/F PXDD/F congeners were determined in four dioxin‐specific bioassays from different species:...
While circulating GH concentrations are high in fetal life, skeletal growth is only slightly reduced by deficiency utero. This has been explained the relatively low binding of to hepatic tissue, suggesting a lack receptors (GHR). The GHR also recognizes ovine placental lactogen (oPL), which may have specific role either as growth-promoting hormone or regulating metabolism. We investigated expression and membrane (o) oPL various tissues maternal liver at different gestational stages....
The S100 proteins MRP8 and MRP14 have been shown to be expressed by myeloid cells during inflammatory reactions. Since the majority of exhibit their biological activity when associated as complex it was investigated whether murine form heterodimers this may bind lipids cell membrane. This is particular importance since anchoring into plasma membrane unclear although upon calcium binding translocate from cytoplasma cytoskeleton Using recombinant we could show that not monomers but only...
Titanium dioxide (TiO₂) is one of the most applied nanomaterials and widely used in food non-food industries as an additive or coating material (E171). It has been shown that E171 contains up to 37% particles which are smaller than 100 nm TiO₂ nanoparticles (NPs) induce cytotoxicity inflammation. Using a nuclear factor Kappa-light-chain enhancer activated B cells (NF-κB) reporter cell line (Caco-2nfkb-RE), Real time polymerase chain reaction (PCR), inhibition dynamin clathrin, it was...
Abstract BACKGROUND The aim of the present study was to generate and identify potential anti‐inflammatory peptides from bovine β‐casein with enzyme preparations cod hog. Furthermore, trypsin, derived fishery by‐products, produce these bioactive for replacement non‐food‐grade tosyl phenylalanyl chloromethyl ketone ( TPCK )‐treated porcine trypsin preparation evaluated. RESULTS Potential were obtained by hydrolysis tryptic ‐treated) a chymotrypsin PTN 6.0 S). Proteolysates generated containing...
S100A9, also referred to as MRP14, is a calcium-binding protein whose expression tightly regulated during differentiation of myeloid cells. The present study was performed the cell type- and differentiation-specific transcriptional regulation S100A9 gene. Analysis promoter in MonoMac-6 cells revealed evidence for novel regulatory region from position -400 -374 bp, termed myeloid-related element (MRE). MRE deletion resulted 5.2-fold reduction activity. By electrophoretic mobility shift...
Cheese, especially ripened varieties, harbor a very complex and heterogeneous microbiota. In addition to the desired microorganisms (starter cultures) added during cheese production, potentially harmful bacteria may also enter production chain. Regarding latter, focus of this study was on coagulase-negative staphylococci (CNS) Macrococcuscaseolyticus. Both are known variety genes coding for antibiotic resistance, including mecA, mecB, mecC, mecD. Coagulase-negative or macrococci carrying...