A5058556925- Malaria Research and Control
- Mosquito-borne diseases and control
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- HIV Research and Treatment
- Research on Leishmaniasis Studies
- Glycosylation and Glycoproteins Research
- Protist diversity and phylogeny
- Invertebrate Immune Response Mechanisms
- Micro and Nano Robotics
- Hepatitis B Virus Studies
- Proteins in Food Systems
- Legume Nitrogen Fixing Symbiosis
- Trypanosoma species research and implications
- Neurogenesis and neuroplasticity mechanisms
- Pancreatic function and diabetes
- Complement system in diseases
- Vector-borne infectious diseases
- Vibrio bacteria research studies
- Endoplasmic Reticulum Stress and Disease
- Hepatitis C virus research
- Fetal and Pediatric Neurological Disorders
- Nerve injury and regeneration
- Toxoplasma gondii Research Studies
- Cellular transport and secretion
Centre National de la Recherche Scientifique
2005-2022
Centre d'Immunologie et des Maladies Infectieuses
2020-2022
Inserm
2009-2022
Sorbonne Université
2009-2022
Institut Pasteur
2005-2017
Neurosciences Paris-Seine
2016
Assistance Publique – Hôpitaux de Paris
2009
Hôpital Armand-Trousseau
2009
Biologie des interactions hôte-parasite
2005
Biologie et Génétique des Interactions Plante-Parasite
2005
Cilia and flagella are complex organelles made of hundreds proteins highly variable structures functions. Here we report the purification intact from procyclic stage Trypanosoma brucei using mechanical shearing. Structural preservation was confirmed by transmission electron microscopy that showed still contained typical elements such as membrane, axoneme, paraflagellar rod, intraflagellar transport particles. It also revealed severed below basal body, were not contaminated other cytoskeletal...
A novel method was validated for the efficient distinction between malaria parasite-derived and host cell proteins in mass spectrometry analyses. This applied to a ghost fraction from Plasmodium falciparum-infected erythrocytes containing red blood plasma membrane, erythrocyte submembrane skeleton, Maurer's clefts, Golgi-like apparatus linked addressing parasite surface. allowed identification of 78 proteins. Among these we identified seven clefts based on immunofluorescence studies...
Plasmodium sporozoites that are transmitted by blood-feeding female Anopheles mosquitoes invade hepatocytes for an initial round of intracellular replication, leading to the release merozoites and multiply within red blood cells. Sporozoites share a number proteins expressed both stages, including Apical Membrane Antigen 1 (AMA1) Rhoptry Neck Proteins (RONs). Although AMA1 RONs essential merozoite invasion erythrocytes during asexual stage replication parasite, their function in was still...
The malarial parasite Plasmodium falciparumTransposes a Golgi-like compartment, referred to as Maurer's clefts, into the cytoplasm of its host cell, erythrocyte, and delivering molecules cell surface. We report here novel role clefts implicating protein phosphatase 1 (PP1) related phosphorylation status P. falciparum skeleton-binding (PfSBP1), trans-membrane interacting with membrane via carboxy-terminal domain. Based on coimmunoprecipitation inhibition studies, we show that PP1 type...
Sporozoite forms of the Plasmodium parasite, causative agent malaria, are transmitted by mosquitoes and first infect liver for an initial round replication before parasite proliferation in blood. The molecular mechanisms involved during sporozoite invasion hepatocytes remain poorly understood. Two receptors Hepatitis C virus (HCV), tetraspanin CD81 scavenger receptor class B type 1 (SR-B1), play important role entry sporozoites into hepatocytes. In contrast to HCV entry, which requires both...
Background Information Eukaryotic cilia and flagella are sophisticated organelles composed of several hundreds proteins that need to be incorporated at the right time place during assembly. Results Two methods were used investigate this process in model protist Trypanosoma brucei : inducible expression epitope‐tagged labelled fluorescence recovery after photobleaching fluorescent fusion proteins. This revealed skeletal components radial spokes (RSP3), central pair (PF16) outer dynein arms...
Abstract Plasmodium sporozoites that are transmitted by blood-feeding female Anopheles mosquitoes invade hepatocytes for an initial round of intracellular replication, leading to the release merozoites and multiply within red blood cells. Sporozoites share a number proteins expressed both stages, including Apical Membrane Antigen 1 (AMA1) Rhoptry Neck Proteins (RONs). Although AMA1 RONs essential merozoite invasion erythrocytes during asexual stage replication parasite, their function in is...
Cilia and flagella are complex organelles composed of up to 500 proteins. We have purified intact from the model organism Trypanosoma brucei using mechanical shearing. Scanning transmission electron microscopy confirmed quality purity biochemical analysis demonstrated a 15-fold enrichment flagellar markers. Mass spectrometry investigation carried out on 5 separate experiments led identification 387 proteins, 55 which had never reported be associated flagellum. 10 12 proteins investigated...
ABSTRACT Sporozoite forms of the malaria parasite Plasmodium are transmitted by mosquitoes and first infect liver for an initial round replication before proliferation in blood. The molecular mechanisms involved during sporozoite invasion hepatocytes remain poorly understood. Two receptors Hepatitis C virus (HCV), tetraspanin CD81 scavenger receptor class B type 1 (SR-B1), play important role entry sporozoites into hepatocytic cells. In contrast to HCV entry, which requires both SR-B1...