A5044509729- Trypanosoma species research and implications
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- Microtubule and mitosis dynamics
- Pancreatic function and diabetes
- Advanced Fluorescence Microscopy Techniques
- Genetic and Kidney Cyst Diseases
- Micro and Nano Robotics
- CRISPR and Genetic Engineering
- Studies on Chitinases and Chitosanases
- Lysosomal Storage Disorders Research
- Cardiomyopathy and Myosin Studies
- Biochemical and Molecular Research
- Cardiac Arrhythmias and Treatments
- Physiological and biochemical adaptations
- Galectins and Cancer Biology
- Electron Spin Resonance Studies
- Photosynthetic Processes and Mechanisms
- Hematopoietic Stem Cell Transplantation
- Complement system in diseases
- Acute Myeloid Leukemia Research
- Viral Infectious Diseases and Gene Expression in Insects
- Synthesis and Biological Evaluation
- RNA modifications and cancer
- Mosquito-borne diseases and control
Ludwig-Maximilians-Universität München
2024-2025
University of Würzburg
2015-2019
Institut Pasteur
2010-2019
Inserm
2015-2019
Centre National de la Recherche Scientifique
2011-2013
Cilia and flagella are complex organelles made of hundreds proteins highly variable structures functions. Here we report the purification intact from procyclic stage Trypanosoma brucei using mechanical shearing. Structural preservation was confirmed by transmission electron microscopy that showed still contained typical elements such as membrane, axoneme, paraflagellar rod, intraflagellar transport particles. It also revealed severed below basal body, were not contaminated other cytoskeletal...
Developmental differentiation is a universal biological process that allows cells to adapt different environments perform specific functions. African trypanosomes progress through tightly regulated life cycle in order survive host when they shuttle between an insect vector and vertebrate host. Transcriptomics has been useful gain insight into RNA changes during stage transitions; however, levels are only moderate proxy for protein abundance trypanosomes. We quantified 4270 groups from the...
The protozoan parasite Trypanosoma brucei is responsible for sleeping sickness and alternates between mammal tsetse fly hosts, where it has to adapt different environments. We investigated the role of two members ALBA family, which encodes hypothetical RNA-binding proteins conserved in most eukaryotes. show that ALBA3/4 colocalize with DHH1 protein a subset poly(A+) RNA stress granules upon starvation. Depletion by interference cultured procyclic stage produces cell modifications mimicking...
African trypanosomes are flagellated protozoan parasites that cause sleeping sickness and transmitted by the bite of tsetse fly. To complete their life cycle in insect, reach salivary glands transform into metacyclic infective form. The latter expelled with saliva at each blood meal during whole insect. Here, we reveal a means which continuous production could be ensured. Dividing present infected flies were monitored live video-microscopy quantitative immunofluorescence analysis using...
RNP granules are ribonucleoprotein assemblies that regulate the post-transcriptional fate of mRNAs in all eukaryotes. Their exact function remains poorly understood, one reason for this is granule purification has not yet been achieved. We have exploited a unique feature trypanosomes to prepare cellular fraction highly enriched starvation stress granules. First, remain trapped within cage-like, subpellicular microtubule array trypanosome cytoskeleton while soluble proteins washed away....
African trypanosomes are flagellated protozoan parasites responsible for sleeping sickness and transmitted by tsetse flies. The accomplishment of their parasite cycle requires adaptation to highly diverse environments. These transitions take place in a strictly defined order accompanied spectacular morphological modifications cell size, shape positioning organelles. To understand the molecular bases these processes, isolated from different tissues fly were analysed immunofluorescence with...
The highly motile and versatile protozoan pathogen Trypanosoma brucei undergoes a complex life cycle in the tsetse fly. Here we introduce host insect as an expedient model environment for microswimmer research, it allows examination of microbial motion within diversified, secluded yet microscopically tractable space. During their week-long journey through different microenvironments fly´s interior organs, incessantly swimming trypanosomes cross various barriers confined surroundings, with...
For persistent infections of the mammalian host, African trypanosomes limit their population size by quorum sensing parasite-excreted stumpy induction factor (SIF), which induces development to tsetse-infective stage. We found that besides this cell density-dependent mechanism, there exists a second path stage is linked antigenic variation, main instrument parasite virulence. The expression variant surface glycoprotein (VSG) leads transcriptional attenuation VSG site (ES) and immediate...
African trypanosomes cause sleeping sickness in humans and nagana cattle. These unicellular parasites are transmitted by the bloodsucking tsetse fly. In mammalian host's circulation, proliferating slender stage cells differentiate into cell cycle-arrested stumpy when they reach high population densities. This transition is thought to fulfil two main functions: first, it auto-regulates parasite load host; second, regarded as only capable of successful vector transmission. Here, we show that...
Abstract Antigenic variation is an immune evasion strategy used by many different pathogens. It involves the periodic, non-random switch in expression of antigens throughout infection. How observed hierarchy antigen achieved has remained a mystery. A key challenge uncovering this process been inability to track transcriptome changes and potential genomic rearrangements individual cells during event. Here, we report establishment highly sensitive single-cell RNA-seq (scRNA-seq) approach for...
Abstract Antigenic variation is an immune evasion strategy used by many different pathogens. It involves the periodic, non-random switch in expression of antigens throughout infection. How observed hierarchy antigen achieved has remained a mystery 1,2 . A key challenge uncovering this process been inability to track transcriptome changes and potential genomic rearrangements individual cells during event. Here we report establishment highly sensitive single-cell RNA sequencing approach for...
The Trypanosoma brucei flagellum is an essential organelle anchored along the surface of cell body via a specialized structure called attachment zone (FAZ). Adhesion relies on interaction extracellular portion two transmembrane proteins termed FLA1 and FLA1BP. Analysis proteome identified FLAM3, novel large protein associated to skeleton whose ablation inhibits attachment. FLAM3 does not contain domains its flagellar localization matches closely but exactly with that paraflagellar rod,...
African trypanosomes are flagellated parasites that cause sleeping sickness. Parasites transmitted from one mammalian host to another by the bite of a tsetse fly. Trypanosoma brucei possesses three different genes for arginine kinase (AK) including (AK3) encodes protein localised flagellum. AK3 is characterised presence unique amino-terminal insertion specifies flagellar targeting. We show here phylogenetic analysis revealing AK arose in two independent duplication events T. and congolense,...
Research on trypanosomes as a model organism has provided substantial contribution to detailed understanding of basic cellular processes within the last few years. At same time, major advances in super-resolution microscopy have been achieved, facilitating resolution biological structures living cells at scale nm. However, motility prevented access high live cells. Here, we present hydrogel based poly(ethylene glycol) functionalized with either norbornene or thiol moieties for UV induced...
The protozoan parasite Trypanosoma brucei is the causative agent of human African trypanosomiasis (HAT). disease fatal if it remains untreated, whereas most drug treatments are inadequate due to high toxicity, difficulties in administration, and low central nervous system penetration. T. glycogen synthase kinase 3 short (TbGSK3s) essential for survival thus represents a potential target that could be exploited HAT treatment. Indirubins, effective leishmanicidals, provide versatile scaffold...
Recent developments in super-resolution microscopy make it possible to resolve structures biological cells at a spatial resolution of few nm and observe dynamical processes with temporal ms μs. However, the optimal structural requires repeated illumination cycles is thus limited chemically fixed cells. For live cell applications substantial improvement over classical Abbe-limited imaging can already be obtained adherent or slow moving Nonetheless, large group are fast could not yet addressed...
Abstract African trypanosomes cause sleeping sickness in humans and nagana cattle. These unicellular parasites are transmitted by the bloodsucking tsetse fly. In mammalian host’s circulation, tissues, interstitium, at least two main life cycle stages exist: slender stumpy bloodstream stages. Proliferating stage cells differentiate into cell cycle-arrested high population densities. This developmental transition occurs response to quorum sensing factor SIF (stumpy induction factor), is...
Cilia and flagella are complex organelles composed of up to 500 proteins. We have purified intact from the model organism Trypanosoma brucei using mechanical shearing. Scanning transmission electron microscopy confirmed quality purity biochemical analysis demonstrated a 15-fold enrichment flagellar markers. Mass spectrometry investigation carried out on 5 separate experiments led identification 387 proteins, 55 which had never reported be associated flagellum. 10 12 proteins investigated...