A5092224895- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Biochemical and Molecular Research
- Mycobacterium research and diagnosis
- HIV/AIDS drug development and treatment
Translational Health Science and Technology Institute
2023-2024
Jawaharlal Nehru University
2023-2024
Abstract The long-term survival of Mtb mandates judicious utilization the available resources inside host. Uninterrupted access to host-derived nutrients holds key success Mtb. Phosphoglucomutase enzyme besides synthesizing glycogen, which serves as a nutrient reservoir, also helps modulate carbon flux in different pathogens. Studies on role glycogen metabolism disease progression, reactivation, and drug susceptibility tuberculosis are severely lacking. To investigate this, we generated an...
Abstract Methionine is essential for the survival of Mycobacterium tuberculosis ( M. ) inside host. However, transsulfuration pathway, a major contributor methionine, dispensable growth suggesting redundancy in methionine biosynthesis pathway. Orthologues MetZ TB other bacterial species are known to operate redundant single-step pathway called direct sulfhydrylation. In this study, we demonstrate that genetic disruption metZ -mediated sulfhydrylation hinders at low pH, an effect mitigated by...
Abstract To overcome the drug resistance crisis and shorten current duration of human tuberculosis (TB) therapy, new anti-TB molecules is required. In an earlier study, we have shown that Mycobacterium ( Mtb ), causative agent TB, with a fractured de novo histidine biosynthesis fails to mount TB infection in mouse model, emboldening disrupting function this pathway may constitute novel strategy curtailing infection. through target based approach designed number triazole scaffold specific...
Abstract Mycobacterium tuberculosis (Mtb) has evolved sophisticated surveillance mechanisms to regulate and neutralize redox imbalances associated lethal consequences. Failing this, the accumulated ROS induces toxicity by oxidizing a variety of biological molecules including proteins, nucleic acids lipids. In present study we identified Mtb’s Rv0495c gene as an important regulator oxidized cytosolic environment. Compared wild type Mtb strain lacking gene, Δ , had increased NAD + /NADH ratio...