A5101533400- Tuberculosis Research and Epidemiology
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Mycobacterium research and diagnosis
- Machine Learning in Bioinformatics
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Research on Leishmaniasis Studies
- Signaling Pathways in Disease
- Amino Acid Enzymes and Metabolism
- RNA modifications and cancer
- Synthesis of β-Lactam Compounds
- Protein Structure and Dynamics
- Click Chemistry and Applications
- Cancer Research and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Toxin Mechanisms and Immunotoxins
- Trypanosoma species research and implications
- Glycosylation and Glycoproteins Research
- Boron and Carbon Nanomaterials Research
- Heat shock proteins research
- Vehicle Noise and Vibration Control
- Escherichia coli research studies
Centre for DNA Fingerprinting and Diagnostics
2019-2024
Manipal Academy of Higher Education
2020-2024
National Institute of Immunology
2012-2024
University of Michigan–Ann Arbor
2023-2024
Indian Institute of Technology Kharagpur
2007-2022
ESIC Hospital
2019
North Bengal Medical College and Hospital
2014
Centre for Cellular and Molecular Biology
2012
University of Nebraska–Lincoln
2008
In mycobacteria, polyketide synthases and nonribosomal peptide synthetases (NRPSs) produce complex lipidic metabolites by using a thio-template mechanism of catalysis. this study, we demonstrate that off-loading reductase (R) domain mycobacterial NRPSs performs two consecutive [2 + 2]e - reductions to release thioester-bound lipopeptides as corresponding alcohols, nonprocessive The first crystal structure an R from Mycobacterium tuberculosis NRPS provides strong support mechanistic model...
Photoelectron spectroscopy (PES) is combined with theoretical calculations to investigate the electronic and atomic structures of three doped aluminum clusters, MAl12− (M=Li, Cu, Au). Well-resolved PES spectra have been obtained at two detachment photon energies, 266nm (4.661eV) 193nm (6.424eV). Basin-hopping global optimization method in combination density-functional theory has used for structural searches. Good agreement between measured simulations helps identify minimum structures. It...
Soil salinity problems are widespread around the globe with increased risk of spreading over years. The fungus Piriformospora indica, identified in Indian Thar desert, colonizes roots monocotyledon plants and provides resistance towards biotic as well abiotic stress conditions. We have a cyclophilin A-like protein from P. indica (PiCypA), which shows higher expression levels during stress. transgenic tobacco overexpressing PiCypA develop osmotic tolerance exhibit normal growth under crystal...
Various monocyclic β-lactams, both cis and trans, have been successfully prepared via Kinugasa reaction mimicking the click chemistry conditions.
Bacterial peptidyl-tRNA hydrolase (Pth; EC 3.1.1.29) hydrolyzes the peptidyl-tRNAs accumulated in cytoplasm and thereby prevents cell death by alleviating tRNA starvation. X-ray NMR studies of Vibrio cholerae Pth (VcPth) mutants its key residues involved catalysis show that activity selectivity protein depends on stereochemistry dynamics H24, D97, N118, N14. D97-H24 interaction is critical for because it increases nucleophilicity H24. The N118 N14 have orthogonally competing interactions...
Rv3488 of Mycobacterium tuberculosis H37Rv has been assigned to the phenolic acid decarboxylase repressor (PadR) family transcriptional regulators that play key roles in multidrug resistance and virulence prokaryotes. The binding cadmium, zinc, several other metals was discovered characterized by isothermal titration calorimetery be an exothermic process. Crystal structures apo-Rv3488 complex with cadmium or zinc ions were determined X-ray crystallography. structure revealed a dimeric...
Abstract Iron is an essential element for Mycobacterium tuberculosis; it has at least 40 enzymes that require iron as a cofactor. Accessibility of the phagosomal surface inside macrophage crucial survival and virulence M. tuberculosis. ESAT-6, 6-kDa-secreted protein region difference 1, known to play role in pathogenesis In our earlier study, we demonstrated ESAT-6 interacts with β-2-microglobulin (β2M) affects class I Ag presentation through sequestration β2M endoplasmic reticulum, which...
Cyclophilins are widely distributed both in eukaryotes and prokaryotes have a primary role as peptidyl-prolyl cis–trans isomerases (PPIases). This study focuses on the cloning, expression, purification crystallization of salinity-stress-induced cyclophilin A (CypA) homologue from symbiotic fungus Piriformospora indica. Crystallization experiments presence 56 mM sodium phosphate monobasic monohydrate, 1.34 M potassium dibasic pH 8.2 yielded crystals that were suitable for X-ray diffraction...
Abstract Background Small airways disease (SAD) is a major cause of airflow obstruction in COPD patients and has been identified as precursor to emphysema. Although the amount SAD lungs can be quantified using our Parametric Response Mapping (PRM) approach, full breadth this readout measure emphysema progression yet explored. We evaluated topological features PRM-derived normal parenchyma surrogates predictors spirometric decline. Methods PRM metrics lung (PRM Norm ) functional fSAD were...
Assessment and selection of donor lungs remains largely subjective experience based. Criteria to accept or decline are poorly standardized not compliant with the current pool. Using ex vivo CT images, we investigated use a CT-based machine learning algorithm for screening prior transplantation.Clinical measures ex-situ scans were collected from 100 cases as part prospective clinical trial. Following procurement, inflated, placed on ice according routine practice, imaged using scanner...
Many Gram-negative bacteria are characterized by hair-like proteinaceous appendages on their surface known as fimbriae. In uropathogenic strains of Escherichia coli, fimbriae mediate attachment binding to receptors the host cell, often contributing virulence and disease. E. coli PapD-like protein (EcpD) is a periplasmic chaperone that plays an important role in proper folding guiding Yad fimbrial proteins outer membrane usher process pilus biogenesis. EcpD essential for biogenesis virulence....