A5028094666- Protein Hydrolysis and Bioactive Peptides
- Meat and Animal Product Quality
- Spectroscopy and Chemometric Analyses
- Insect Utilization and Effects
- Phytochemicals and Antioxidant Activities
- Proteins in Food Systems
- Natural product bioactivities and synthesis
- Biochemical effects in animals
- Neuropeptides and Animal Physiology
- Phytochemistry and Biological Activities
- Peptidase Inhibition and Analysis
- Natural Antidiabetic Agents Studies
- Animal Nutrition and Physiology
- Collagen: Extraction and Characterization
- Muscle metabolism and nutrition
- Plant biochemistry and biosynthesis
- Aquaculture Nutrition and Growth
- Protein purification and stability
- Spectroscopy Techniques in Biomedical and Chemical Research
- Metabolomics and Mass Spectrometry Studies
- Carbohydrate Chemistry and Synthesis
- Bioactive Compounds and Antitumor Agents
- Traditional Chinese Medicine Analysis
- Plant-based Medicinal Research
- Fungal Biology and Applications
Nofima
2017-2025
Fafo Foundation
2017-2025
University of Copenhagen
2011-2019
Association for Symbolic Logic
2017
Institute of Fisheries and Aquaculture
2017
University of Bergen
2012
Université Lille Nord de France
2011
Unité de catalyse et de chimie du solide de Lille
2011
While the harmonized INFOGEST model provides a physiologically relevant platform for simulated digestion, it needs to be combined with adequate analytical methods enable quantification and comparison of protein digestibility in different food matrices. We have shown that size exclusion chromatography (SEC) can used estimate proportion small peptides potentially available uptake. Combined determination total dissolved protein, % per was calculated as (DSEC). Values DSEC differed casein...
Extracts of five halophytes from southern Portugal (Arthrocnemum macrostachyum, Mesembryanthemum edule, Juncus acutus, Plantago coronopus and Halimione portulacoides), were studied for antioxidant, anti-inflammatory in vitro antitumor properties. The most active extracts towards the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical methanol M. edule (IC50 = 0.1 mg/mL) J. acutus 0.4 mg/mL), ether 0.2 A. macrostachyum 0.3 mg/mL). highest scavenging activity (RSA) against 2,2′-azino-bis...
Significance Ingenol mebutate is a diterpene ester with highly complex macrocyclic structure that has been approved for the treatment of actinic keratosis, precondition skin cancer. The current production ingenol through plant extraction or chemical synthesis inefficient and costly. Here, we describe discovery biosynthetic route in Euphorbia lathyris L. (caper spurge) which regio-specific oxidation casbene followed by an unconventional cyclization to yield jolkinol C, probable key...
A bioanalytical platform combining magnetic ligand fishing for α-glucosidase inhibition profiling and HPLC-HRMS-SPE-NMR structural identification of inhibitory ligands, both directly from crude plant extracts, is presented. Magnetic beads with N-terminus-coupled were synthesized characterized their inherent catalytic activity. Ligand the immobilized enzyme was optimized using an artificial test mixture consisting caffeine, ferulic acid, luteolin before proof-of-concept extract Eugenia...
An FTIR-based multivariate approach is developed for monitoring molecular weight distribution during enzymatic protein hydrolysis of byproducts.
Metabolic disorders are increasingly prevalent conditions that manifest pathophysiologically along a continuum. Among reported metabolic risk factors, elevated fasting serum glucose (FSG) levels have shown the most substantial increase in exposure. Ultimately leading to insulin resistance (IR), this condition is associated with notable deteriorations prognostic outlook for major diseases, including neurodegenerative cancer risk, and mortality related cardiovascular disease. Tackling...
The potential of dry-film Fourier-transform infrared (FTIR) measurements as a monitoring tool for enzymatic hydrolysis protein-based substrates is explored in this study.
According to the International Diabetes Federation, type 2 diabetes (T2D) has reached epidemic proportions, affecting more than 382 million people worldwide. Inhibition of protein tyrosine phosphatase-1B (PTP1B) and α-glucosidase is a recognized therapeutic approach for management T2D its associated complications. The lack clinical drugs targeting PTP1B side effects existing drugs, emphasize need new drug leads these targets. In present work, dual high-resolution inhibition profiles...
Type 2 diabetes is a metabolic disorder affecting millions of people worldwide, and new drug leads or functional foods containing selective α-glucosidase inhibitors are needed. Crude extract 24 plants were assessed for inhibitory activity. Methanol extracts Cinnamomum zeylanicum bark, Rheum rhabarbarum peel, palmatum root ethyl acetate C. Allium ascalonicum R. showed IC50 values below 20 μg/mL. Subsequently, high-resolution profiling was used in combination with high-performance liquid...
In the presented study, Fourier-transform infrared (FTIR) spectroscopy is used to predict average molecular weight of protein hydrolysates produced from protein-rich by-products food industry using commercial enzymes. Enzymatic hydrolysis a well-established method for production formulations, recognized its potential valorize food-processing by-products. The monitoring such processes still significant challenge as existing classical analytical methods are not easily applicable industrial...
Fourier transform infrared spectroscopy (FTIR) is a powerful analytical tool that has been used for protein and peptide characterization decades. In the present study, objective was to investigate if FTIR can be predict collagen content in hydrolyzed samples. All samples were obtained from enzymatic hydrolysis (EPH) of poultry by-products providing span 0.3% 37.9% (dry weight), analysis performed using dry film FTIR. Since nonlinear effects revealed by calibration standard partial least...
Industries are continuously looking for dedicated sensor solutions evaluating the chemical composition of products that can provide valuable insights into process control, optimization, and product quality. A rapid robust analytical method, Fourier-Transform Infrared (FTIR) spectroscopy, holds significant potential in this regard as it provides detailed compositional values food nutrients. The main aim present study was to use dry film FTIR spectroscopy an tool characterize from industrial...
Crossflow membrane separation was used as a scalable downstream process for the up concentrate of low-molecular-weight peptides and removal salt (NaCl) from Calanus finmarchicus hydrolysate. Membrane processes are increasingly various applications in both upstream processing. The C. hydrolysate prepared by enzymatic hydrolysis, followed crossflow separation. stepwise nanofiltration contributed to progressive reduction concentration concentrates decreased 34%, 53%, 75%, highlighting...
Abstract In this study we explore the potential of using Fourier-transform infrared (FTIR) spectra trifluoroacetate-protein and peptide complexes for monitoring proteolytic reactions. The idea treating dry-films protein hydrolysates with trifluoroacetic acid (TFA) prior to FTIR analysis is based on unique properties TFA. By adding a large excess TFA hydrolysate samples, possible protonation sites proteins peptides will be saturated. addition, has low boiling point when protonated as well...
An extract of Carthamus oxyacantha (wild safflower) was investigated using two approaches: a traditional, nontarget fractionation by VLC and HPLC, the hyphenated technique HPLC-PDA-HRMS-SPE-NMR followed targeted isolation selected constituents for inclusion in screening library pure natural products. While involved considerable time spent on pursuing fractions containing well-known or undesired compounds, analysis considerably faster required less solvent other consumables. The results were...